Characteristics of intestinal microbiota in infants with late-onset breast milk jaundice
IntroductionIn this paper, microbiota analysis was determined to analyze the structure and difference of intestinal microbiota between LBMJ (late-onset breast milk jaundice) infants and healthy individuals.MethodsWe collected fresh fecal samples from 13 infants with LBMJ and 13 healthy individuals,...
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Frontiers Media S.A.
2023-05-01
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| Series: | Frontiers in Nutrition |
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| Online Access: | https://www.frontiersin.org/articles/10.3389/fnut.2023.1119768/full |
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| author | Qianying Guo Xinran Liu Mingxuan Cui Xuening Li Chen Yang Shilong Zhao Lina Pan Xiaoyu Peng Linlin Wang Peng Liu |
| author_facet | Qianying Guo Xinran Liu Mingxuan Cui Xuening Li Chen Yang Shilong Zhao Lina Pan Xiaoyu Peng Linlin Wang Peng Liu |
| author_sort | Qianying Guo |
| collection | DOAJ |
| description | IntroductionIn this paper, microbiota analysis was determined to analyze the structure and difference of intestinal microbiota between LBMJ (late-onset breast milk jaundice) infants and healthy individuals.MethodsWe collected fresh fecal samples from 13 infants with LBMJ and 13 healthy individuals, then determined the intestinal microbiota by 16 s rRNA sequencing. The differences of microbiota structure, diversity and functional characteristics between the two groups were analyzed, and the correlation between dominant genus and TcB (transcutaneous bilirubin) value was calculated.ResultsIn this study, there were no significant differences in maternal demographic characteristics, neonatal status and macronutrients in breast milk between the two groups (p > 0.05). There are differences in the structure of intestinal microbiota between LBMJ and the control group. At the genus level, the relative abundance of Klebsiella in the case group is high (p < 0.05). At the same time, correlation analysis indicates that the abundance of Klebsiella is positively correlated with TcB value. The intestinal microbiota richness and diversity (Alpha diversity and Beta diversity) of the two groups were significantly different (p < 0.05). LEfSe analysis showed that 25 genera including Klebsiella was significantly enriched in the LBMJ infants, and the other 17 species are enriched in the control group. Functional prediction analysis indicated that 42 metabolic pathways may be related to the occurrence of LBMJ.ConclusionIn conclusion, characteristic changes are seen in intestinal microbiota compositions between LBMJ infants and the healthy controls. Klebsiella is closely associated with the severity of the disease, which may be due to enhanced β-glucuronidase activity. |
| first_indexed | 2024-04-09T13:13:33Z |
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| id | doaj.art-835c3c3985cd4809a6f0e7dcae3241c0 |
| institution | Directory Open Access Journal |
| issn | 2296-861X |
| language | English |
| last_indexed | 2024-04-09T13:13:33Z |
| publishDate | 2023-05-01 |
| publisher | Frontiers Media S.A. |
| record_format | Article |
| series | Frontiers in Nutrition |
| spelling | doaj.art-835c3c3985cd4809a6f0e7dcae3241c02023-05-12T05:16:11ZengFrontiers Media S.A.Frontiers in Nutrition2296-861X2023-05-011010.3389/fnut.2023.11197681119768Characteristics of intestinal microbiota in infants with late-onset breast milk jaundiceQianying Guo0Xinran Liu1Mingxuan Cui2Xuening Li3Chen Yang4Shilong Zhao5Lina Pan6Xiaoyu Peng7Linlin Wang8Peng Liu9Department of Clinical Nutrition, Peking University People’s Hospital, Beijing, ChinaDepartment of Clinical Nutrition, Peking University People’s Hospital, Beijing, ChinaDepartment of Clinical Nutrition, Peking University People’s Hospital, Beijing, ChinaDepartment of Obstetrics and Gynecology, Peking University Third Hospital, Beijing, ChinaNational Health Commission Key Laboratory of Reproductive Health, School of Public Health, Institute of Reproductive and Child Health, Peking University, Beijing, ChinaDepartment of Clinical Nutrition, Peking University People’s Hospital, Beijing, ChinaAusnutria Dairy (China) Co., Ltd., Changsha, ChinaAusnutria Dairy (China) Co., Ltd., Changsha, ChinaNational Health Commission Key Laboratory of Reproductive Health, School of Public Health, Institute of Reproductive and Child Health, Peking University, Beijing, ChinaDepartment of Clinical Nutrition, Peking University People’s Hospital, Beijing, ChinaIntroductionIn this paper, microbiota analysis was determined to analyze the structure and difference of intestinal microbiota between LBMJ (late-onset breast milk jaundice) infants and healthy individuals.MethodsWe collected fresh fecal samples from 13 infants with LBMJ and 13 healthy individuals, then determined the intestinal microbiota by 16 s rRNA sequencing. The differences of microbiota structure, diversity and functional characteristics between the two groups were analyzed, and the correlation between dominant genus and TcB (transcutaneous bilirubin) value was calculated.ResultsIn this study, there were no significant differences in maternal demographic characteristics, neonatal status and macronutrients in breast milk between the two groups (p > 0.05). There are differences in the structure of intestinal microbiota between LBMJ and the control group. At the genus level, the relative abundance of Klebsiella in the case group is high (p < 0.05). At the same time, correlation analysis indicates that the abundance of Klebsiella is positively correlated with TcB value. The intestinal microbiota richness and diversity (Alpha diversity and Beta diversity) of the two groups were significantly different (p < 0.05). LEfSe analysis showed that 25 genera including Klebsiella was significantly enriched in the LBMJ infants, and the other 17 species are enriched in the control group. Functional prediction analysis indicated that 42 metabolic pathways may be related to the occurrence of LBMJ.ConclusionIn conclusion, characteristic changes are seen in intestinal microbiota compositions between LBMJ infants and the healthy controls. Klebsiella is closely associated with the severity of the disease, which may be due to enhanced β-glucuronidase activity.https://www.frontiersin.org/articles/10.3389/fnut.2023.1119768/fulllate-onset breast milk jaundiceintestinal microbiota16S rRNAbreastfed infantsKlebsiella |
| spellingShingle | Qianying Guo Xinran Liu Mingxuan Cui Xuening Li Chen Yang Shilong Zhao Lina Pan Xiaoyu Peng Linlin Wang Peng Liu Characteristics of intestinal microbiota in infants with late-onset breast milk jaundice Frontiers in Nutrition late-onset breast milk jaundice intestinal microbiota 16S rRNA breastfed infants Klebsiella |
| title | Characteristics of intestinal microbiota in infants with late-onset breast milk jaundice |
| title_full | Characteristics of intestinal microbiota in infants with late-onset breast milk jaundice |
| title_fullStr | Characteristics of intestinal microbiota in infants with late-onset breast milk jaundice |
| title_full_unstemmed | Characteristics of intestinal microbiota in infants with late-onset breast milk jaundice |
| title_short | Characteristics of intestinal microbiota in infants with late-onset breast milk jaundice |
| title_sort | characteristics of intestinal microbiota in infants with late onset breast milk jaundice |
| topic | late-onset breast milk jaundice intestinal microbiota 16S rRNA breastfed infants Klebsiella |
| url | https://www.frontiersin.org/articles/10.3389/fnut.2023.1119768/full |
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