CD64 plays a key role in diabetic wound healing
IntroductionWound healing poses a clinical challenge in diabetes mellitus (DM) due to compromised host immunity. CD64, an IgG-binding Fcgr1 receptor, acts as a pro-inflammatory mediator. While its presence has been identified in various inflammatory diseases, its specific role in wound healing, espe...
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| Format: | Article |
| Language: | English |
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Frontiers Media S.A.
2024-03-01
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| Series: | Frontiers in Immunology |
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| Online Access: | https://www.frontiersin.org/articles/10.3389/fimmu.2024.1322256/full |
| _version_ | 1797269050156384256 |
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| author | Xiuqin Zhang Liuhong Yuan Zhenyu Tan Huiyan Wu Feier Chen Junjie Huang Pengjun Wang Brett D. Hambly Shisan Bao Kun Tao |
| author_facet | Xiuqin Zhang Liuhong Yuan Zhenyu Tan Huiyan Wu Feier Chen Junjie Huang Pengjun Wang Brett D. Hambly Shisan Bao Kun Tao |
| author_sort | Xiuqin Zhang |
| collection | DOAJ |
| description | IntroductionWound healing poses a clinical challenge in diabetes mellitus (DM) due to compromised host immunity. CD64, an IgG-binding Fcgr1 receptor, acts as a pro-inflammatory mediator. While its presence has been identified in various inflammatory diseases, its specific role in wound healing, especially in DM, remains unclear.ObjectivesWe aimed to investigate the involvement of CD64 in diabetic wound healing using a DM animal model with CD64 KO mice.MethodsFirst, we compared CD64 expression in chronic skin ulcers from human DM and non-DM skin. Then, we monitored wound healing in a DM mouse model over 10 days, with or without CD64 KO, using macroscopic and microscopic observations, as well as immunohistochemistry.ResultsCD64 expression was significantly upregulated (1.25-fold) in chronic ulcerative skin from DM patients compared to non-DM individuals. Clinical observations were consistent with animal model findings, showing a significant delay in wound healing, particularly by day 7, in CD64 KO mice compared to WT mice. Additionally, infiltrating CD163+ M2 macrophages in the wounds of DM mice decreased significantly compared to non-DM mice over time. Delayed wound healing in DM CD64 KO mice correlated with the presence of inflammatory mediators.ConclusionCD64 seems to play a crucial role in wound healing, especially in DM conditions, where it is associated with CD163+ M2 macrophage infiltration. These data suggest that CD64 relies on host immunity during the wound healing process. Such data may provide useful information for both basic scientists and clinicians to deal with diabetic chronic wound healing. |
| first_indexed | 2024-04-25T01:42:12Z |
| format | Article |
| id | doaj.art-835c97946d8749359e3eb9ae22ccd3ed |
| institution | Directory Open Access Journal |
| issn | 1664-3224 |
| language | English |
| last_indexed | 2024-04-25T01:42:12Z |
| publishDate | 2024-03-01 |
| publisher | Frontiers Media S.A. |
| record_format | Article |
| series | Frontiers in Immunology |
| spelling | doaj.art-835c97946d8749359e3eb9ae22ccd3ed2024-03-08T04:34:28ZengFrontiers Media S.A.Frontiers in Immunology1664-32242024-03-011510.3389/fimmu.2024.13222561322256CD64 plays a key role in diabetic wound healingXiuqin Zhang0Liuhong Yuan1Zhenyu Tan2Huiyan Wu3Feier Chen4Junjie Huang5Pengjun Wang6Brett D. Hambly7Shisan Bao8Kun Tao9Department of Pathology, Tongji Hospital, School of Medicine, Tongji University, Shanghai, ChinaDepartment of Pathology, Tongji Hospital, School of Medicine, Tongji University, Shanghai, ChinaDepartment of Pathology, Tongji Hospital, School of Medicine, Tongji University, Shanghai, ChinaDepartment of Pathology, Tongren Hospital, Shanghai Jiaotong University School of Medicine, Shanghai, ChinaDepartment of Pathology, Tongji Hospital, School of Medicine, Tongji University, Shanghai, ChinaDepartment of Pathology, Tongren Hospital, Shanghai Jiaotong University School of Medicine, Shanghai, ChinaDepartment of Pathology, Tongren Hospital, Shanghai Jiaotong University School of Medicine, Shanghai, ChinaDepartment of Pathology, Tongren Hospital, Shanghai Jiaotong University School of Medicine, Shanghai, ChinaDepartment of Pathology, Tongren Hospital, Shanghai Jiaotong University School of Medicine, Shanghai, ChinaDepartment of Pathology, Tongji Hospital, School of Medicine, Tongji University, Shanghai, ChinaIntroductionWound healing poses a clinical challenge in diabetes mellitus (DM) due to compromised host immunity. CD64, an IgG-binding Fcgr1 receptor, acts as a pro-inflammatory mediator. While its presence has been identified in various inflammatory diseases, its specific role in wound healing, especially in DM, remains unclear.ObjectivesWe aimed to investigate the involvement of CD64 in diabetic wound healing using a DM animal model with CD64 KO mice.MethodsFirst, we compared CD64 expression in chronic skin ulcers from human DM and non-DM skin. Then, we monitored wound healing in a DM mouse model over 10 days, with or without CD64 KO, using macroscopic and microscopic observations, as well as immunohistochemistry.ResultsCD64 expression was significantly upregulated (1.25-fold) in chronic ulcerative skin from DM patients compared to non-DM individuals. Clinical observations were consistent with animal model findings, showing a significant delay in wound healing, particularly by day 7, in CD64 KO mice compared to WT mice. Additionally, infiltrating CD163+ M2 macrophages in the wounds of DM mice decreased significantly compared to non-DM mice over time. Delayed wound healing in DM CD64 KO mice correlated with the presence of inflammatory mediators.ConclusionCD64 seems to play a crucial role in wound healing, especially in DM conditions, where it is associated with CD163+ M2 macrophage infiltration. These data suggest that CD64 relies on host immunity during the wound healing process. Such data may provide useful information for both basic scientists and clinicians to deal with diabetic chronic wound healing.https://www.frontiersin.org/articles/10.3389/fimmu.2024.1322256/fulldiabetes mellitusCD64CD163+ M2 macrophagewound healingCD68/CD80 M1 macrophages |
| spellingShingle | Xiuqin Zhang Liuhong Yuan Zhenyu Tan Huiyan Wu Feier Chen Junjie Huang Pengjun Wang Brett D. Hambly Shisan Bao Kun Tao CD64 plays a key role in diabetic wound healing Frontiers in Immunology diabetes mellitus CD64 CD163+ M2 macrophage wound healing CD68/CD80 M1 macrophages |
| title | CD64 plays a key role in diabetic wound healing |
| title_full | CD64 plays a key role in diabetic wound healing |
| title_fullStr | CD64 plays a key role in diabetic wound healing |
| title_full_unstemmed | CD64 plays a key role in diabetic wound healing |
| title_short | CD64 plays a key role in diabetic wound healing |
| title_sort | cd64 plays a key role in diabetic wound healing |
| topic | diabetes mellitus CD64 CD163+ M2 macrophage wound healing CD68/CD80 M1 macrophages |
| url | https://www.frontiersin.org/articles/10.3389/fimmu.2024.1322256/full |
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