A Phenotypic Search on Graft Compatibility in Grapevine
Grafting is the most used propagation method in viticulture and is the unique control strategy against Phylloxera. Nevertheless, its practice remains limited mainly due to inconsistent graft success and difficulties in predicting graft compatibility responses of proposed scion–rootstock combinations...
| Main Authors: | , , , |
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| Format: | Article |
| Language: | English |
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MDPI AG
2020-05-01
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| Series: | Agronomy |
| Subjects: | |
| Online Access: | https://www.mdpi.com/2073-4395/10/5/706 |
| _version_ | 1827716861139091456 |
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| author | Sara Tedesco Ana Pina Pedro Fevereiro Friedrich Kragler |
| author_facet | Sara Tedesco Ana Pina Pedro Fevereiro Friedrich Kragler |
| author_sort | Sara Tedesco |
| collection | DOAJ |
| description | Grafting is the most used propagation method in viticulture and is the unique control strategy against Phylloxera. Nevertheless, its practice remains limited mainly due to inconsistent graft success and difficulties in predicting graft compatibility responses of proposed scion–rootstock combinations, slowing down the selection of elite rootstocks. Aiming to identify optimal phenotypic parameters related to graft (in)compatibility, we used four clones of two grapevine cultivars that show different compatibility behavior when grafted onto the same rootstock. Several physiological parameters, internal anatomy of the graft union, chlorophyll fluorescence, and pigment contents of homo- and heterografts were monitored in a nursery-grafting context. The measurements highlighted enhanced performance of the heterografts due to rooting difficulties of <i>Vitis vinifera</i> homografts. This suggests that in viticulture, homografts should only be used as compatibility controls regarding qualitative attributes. By observing the internal anatomy of the union, we found that grapevines might require longer times for graft healing than anticipated. While Affinity Coefficients were not informative to assess incompatibility, leaf chlorophyll concentration analysis proved to be a more sensitive indicator of stress than the analysis of chlorophyll fluorescence. Overall, we conclude that graft take correlated best with <i>callus</i> formation at the graft junction three weeks after grafting. |
| first_indexed | 2024-03-10T19:49:44Z |
| format | Article |
| id | doaj.art-8362cc5c502d47d2a6cfcf1e445c7cdf |
| institution | Directory Open Access Journal |
| issn | 2073-4395 |
| language | English |
| last_indexed | 2024-03-10T19:49:44Z |
| publishDate | 2020-05-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Agronomy |
| spelling | doaj.art-8362cc5c502d47d2a6cfcf1e445c7cdf2023-11-20T00:31:07ZengMDPI AGAgronomy2073-43952020-05-0110570610.3390/agronomy10050706A Phenotypic Search on Graft Compatibility in GrapevineSara Tedesco0Ana Pina1Pedro Fevereiro2Friedrich Kragler3Plant Cell Biotechnology Lab, Instituto de Tecnologia Química e Biológica António Xavier (Green-it Unit), Avenida da República, Estação Agronómica Nacional, 2780-157 Oeiras, PortugalUnidad de Hortofruticultura, Centro de Investigación y Tecnología Agroalimentaria de Aragón (CITA), Instituto Agroalimentario de Aragón-IA2 (CITA-Universidad de Zaragoza), Av. Montañana, 930, 50059 Zaragoza, SpainPlant Cell Biotechnology Lab, Instituto de Tecnologia Química e Biológica António Xavier (Green-it Unit), Avenida da República, Estação Agronómica Nacional, 2780-157 Oeiras, PortugalMax Planck Institut für Molekulare Pflanzenphysiologie, Wissenschaftspark Golm, Am Mühlenberg 1, 14476 Potsdam, GermanyGrafting is the most used propagation method in viticulture and is the unique control strategy against Phylloxera. Nevertheless, its practice remains limited mainly due to inconsistent graft success and difficulties in predicting graft compatibility responses of proposed scion–rootstock combinations, slowing down the selection of elite rootstocks. Aiming to identify optimal phenotypic parameters related to graft (in)compatibility, we used four clones of two grapevine cultivars that show different compatibility behavior when grafted onto the same rootstock. Several physiological parameters, internal anatomy of the graft union, chlorophyll fluorescence, and pigment contents of homo- and heterografts were monitored in a nursery-grafting context. The measurements highlighted enhanced performance of the heterografts due to rooting difficulties of <i>Vitis vinifera</i> homografts. This suggests that in viticulture, homografts should only be used as compatibility controls regarding qualitative attributes. By observing the internal anatomy of the union, we found that grapevines might require longer times for graft healing than anticipated. While Affinity Coefficients were not informative to assess incompatibility, leaf chlorophyll concentration analysis proved to be a more sensitive indicator of stress than the analysis of chlorophyll fluorescence. Overall, we conclude that graft take correlated best with <i>callus</i> formation at the graft junction three weeks after grafting.https://www.mdpi.com/2073-4395/10/5/706<i>Vitis</i>grapevinegraftinggraft incompatibilitygraft success predictionrootstock |
| spellingShingle | Sara Tedesco Ana Pina Pedro Fevereiro Friedrich Kragler A Phenotypic Search on Graft Compatibility in Grapevine Agronomy <i>Vitis</i> grapevine grafting graft incompatibility graft success prediction rootstock |
| title | A Phenotypic Search on Graft Compatibility in Grapevine |
| title_full | A Phenotypic Search on Graft Compatibility in Grapevine |
| title_fullStr | A Phenotypic Search on Graft Compatibility in Grapevine |
| title_full_unstemmed | A Phenotypic Search on Graft Compatibility in Grapevine |
| title_short | A Phenotypic Search on Graft Compatibility in Grapevine |
| title_sort | phenotypic search on graft compatibility in grapevine |
| topic | <i>Vitis</i> grapevine grafting graft incompatibility graft success prediction rootstock |
| url | https://www.mdpi.com/2073-4395/10/5/706 |
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