In Vitro and In Vivo Biocompatibility Studies on Engineered Fabric with Graphene Nanoplatelets
To produce clothes made with engineered fabrics to monitor the physiological parameters of workers, strain sensors were produced by depositing two different types of water-based inks (P1 and P2) suitably mixed with graphene nanoplatelets (GNPs) on a fabric. We evaluated the biocompatibility of fabri...
| Main Authors: | , , , , , , , , , , |
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| Format: | Article |
| Language: | English |
| Published: |
MDPI AG
2022-04-01
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| Series: | Nanomaterials |
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| Online Access: | https://www.mdpi.com/2079-4991/12/9/1405 |
| _version_ | 1797503583882575872 |
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| author | Carla Fanizza Mara Stefanelli Anna Risuglia Erika Bruni Federica Ietto Federica Incoronato Fabrizio Marra Adele Preziosi Patrizia Mancini Maria Sabrina Sarto Daniela Uccelletti |
| author_facet | Carla Fanizza Mara Stefanelli Anna Risuglia Erika Bruni Federica Ietto Federica Incoronato Fabrizio Marra Adele Preziosi Patrizia Mancini Maria Sabrina Sarto Daniela Uccelletti |
| author_sort | Carla Fanizza |
| collection | DOAJ |
| description | To produce clothes made with engineered fabrics to monitor the physiological parameters of workers, strain sensors were produced by depositing two different types of water-based inks (P1 and P2) suitably mixed with graphene nanoplatelets (GNPs) on a fabric. We evaluated the biocompatibility of fabrics with GNPs (GNP fabric) through in vitro and in vivo assays. We investigated the effects induced on human keratinocytes by the eluates extracted from GNP fabrics by the contact of GNP fabrics with cells and by seeding keratinocytes directly onto the GNP fabrics using a cell viability test and morphological analysis. Moreover, we evaluated in vivo possible adverse effects of the GNPs using the model system <i>Caenorhabditis elegans</i>. Cell viability assay, morphological analysis and <i>Caenorhabditis elegans</i> tests performed on smart fabric treated with P2 (P2GNP fabric) did not show significant differences when compared with their respective control samples. Instead, a reduction in cell viability and changes in the membrane microvilli structure were found in cells incubated with smart fabric treated with P1. The results were helpful in determining the non-toxic properties of the P2GNP fabric. In the future, therefore, graphene-based ink integrated into elastic fabric will be developed for piezoresistive sensors. |
| first_indexed | 2024-03-10T03:52:47Z |
| format | Article |
| id | doaj.art-8362e54c7e9a454899823e5631bc999c |
| institution | Directory Open Access Journal |
| issn | 2079-4991 |
| language | English |
| last_indexed | 2024-03-10T03:52:47Z |
| publishDate | 2022-04-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Nanomaterials |
| spelling | doaj.art-8362e54c7e9a454899823e5631bc999c2023-11-23T08:53:42ZengMDPI AGNanomaterials2079-49912022-04-01129140510.3390/nano12091405In Vitro and In Vivo Biocompatibility Studies on Engineered Fabric with Graphene NanoplateletsCarla Fanizza0Mara Stefanelli1Anna Risuglia2Erika Bruni3Federica Ietto4Federica Incoronato5Fabrizio Marra6Adele Preziosi7Patrizia Mancini8Maria Sabrina Sarto9Daniela Uccelletti10Department of Technological Innovations and Safety of Plants, Products and Anthropic Settlements (DITSIPIA), National Institute for Insurance against Accidents at Work (INAIL), 00143 Rome, ItalyDepartment of Technological Innovations and Safety of Plants, Products and Anthropic Settlements (DITSIPIA), National Institute for Insurance against Accidents at Work (INAIL), 00143 Rome, ItalyDepartment of Technological Innovations and Safety of Plants, Products and Anthropic Settlements (DITSIPIA), National Institute for Insurance against Accidents at Work (INAIL), 00143 Rome, ItalyDepartment of Biology and Biotechnology C. Darwin, Sapienza University of Rome, 00185 Rome, ItalyDepartment of Technological Innovations and Safety of Plants, Products and Anthropic Settlements (DITSIPIA), National Institute for Insurance against Accidents at Work (INAIL), 00143 Rome, ItalyDepartment of Technological Innovations and Safety of Plants, Products and Anthropic Settlements (DITSIPIA), National Institute for Insurance against Accidents at Work (INAIL), 00143 Rome, ItalyDepartment of Astronautical, Electrical and Energy Engineering, Sapienza University of Rome, 00184 Rome, ItalyDepartment of Biology and Biotechnology C. Darwin, Sapienza University of Rome, 00185 Rome, ItalyDepartment of Experimental Medicine, Sapienza University of Rome, 00161 Rome, ItalyDepartment of Astronautical, Electrical and Energy Engineering, Sapienza University of Rome, 00184 Rome, ItalyDepartment of Biology and Biotechnology C. Darwin, Sapienza University of Rome, 00185 Rome, ItalyTo produce clothes made with engineered fabrics to monitor the physiological parameters of workers, strain sensors were produced by depositing two different types of water-based inks (P1 and P2) suitably mixed with graphene nanoplatelets (GNPs) on a fabric. We evaluated the biocompatibility of fabrics with GNPs (GNP fabric) through in vitro and in vivo assays. We investigated the effects induced on human keratinocytes by the eluates extracted from GNP fabrics by the contact of GNP fabrics with cells and by seeding keratinocytes directly onto the GNP fabrics using a cell viability test and morphological analysis. Moreover, we evaluated in vivo possible adverse effects of the GNPs using the model system <i>Caenorhabditis elegans</i>. Cell viability assay, morphological analysis and <i>Caenorhabditis elegans</i> tests performed on smart fabric treated with P2 (P2GNP fabric) did not show significant differences when compared with their respective control samples. Instead, a reduction in cell viability and changes in the membrane microvilli structure were found in cells incubated with smart fabric treated with P1. The results were helpful in determining the non-toxic properties of the P2GNP fabric. In the future, therefore, graphene-based ink integrated into elastic fabric will be developed for piezoresistive sensors.https://www.mdpi.com/2079-4991/12/9/1405smart fabricsstrain sensorsgraphene nanoplateletsbiocompatibilityHaCaT cellsMTT assay |
| spellingShingle | Carla Fanizza Mara Stefanelli Anna Risuglia Erika Bruni Federica Ietto Federica Incoronato Fabrizio Marra Adele Preziosi Patrizia Mancini Maria Sabrina Sarto Daniela Uccelletti In Vitro and In Vivo Biocompatibility Studies on Engineered Fabric with Graphene Nanoplatelets Nanomaterials smart fabrics strain sensors graphene nanoplatelets biocompatibility HaCaT cells MTT assay |
| title | In Vitro and In Vivo Biocompatibility Studies on Engineered Fabric with Graphene Nanoplatelets |
| title_full | In Vitro and In Vivo Biocompatibility Studies on Engineered Fabric with Graphene Nanoplatelets |
| title_fullStr | In Vitro and In Vivo Biocompatibility Studies on Engineered Fabric with Graphene Nanoplatelets |
| title_full_unstemmed | In Vitro and In Vivo Biocompatibility Studies on Engineered Fabric with Graphene Nanoplatelets |
| title_short | In Vitro and In Vivo Biocompatibility Studies on Engineered Fabric with Graphene Nanoplatelets |
| title_sort | in vitro and in vivo biocompatibility studies on engineered fabric with graphene nanoplatelets |
| topic | smart fabrics strain sensors graphene nanoplatelets biocompatibility HaCaT cells MTT assay |
| url | https://www.mdpi.com/2079-4991/12/9/1405 |
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