Maternal obesity alters C19MC microRNAs expression profile in fetal umbilical cord blood
Abstract Background To determine if overweight/obese pregnant women have altered microRNA expression patterns in fetal umbilical cord blood that may affect the development of offspring. Methods Umbilical cord blood samples were obtained from the fetuses of 34 overweight/obese and 32 normal-weight wo...
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| Format: | Article |
| Language: | English |
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BMC
2020-07-01
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| Series: | Nutrition & Metabolism |
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| Online Access: | http://link.springer.com/article/10.1186/s12986-020-00475-7 |
| _version_ | 1828868769153286144 |
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| author | Jia Jing Yingjin Wang Yanmei Quan Zhijie Wang Yue Liu Zhide Ding |
| author_facet | Jia Jing Yingjin Wang Yanmei Quan Zhijie Wang Yue Liu Zhide Ding |
| author_sort | Jia Jing |
| collection | DOAJ |
| description | Abstract Background To determine if overweight/obese pregnant women have altered microRNA expression patterns in fetal umbilical cord blood that may affect the development of offspring. Methods Umbilical cord blood samples were obtained from the fetuses of 34 overweight/obese and 32 normal-weight women after delivery. Next generation sequencing (NGS) analyzed their miRNA expression patterns. miRanda and TargetScan databases were used to predict the miRNAs’ target genes followed by analyses of Gene ontology (GO) and Kyoto Encyclopedia of Genes and Genomes (KEGG) to perform function grouping and pathway analyses. qRT-PCR verified the identity of differentially expressed miRNAs that were revealed in the NGS results. Results There was a positive correlation between newborn body weight and pregestational BMI of pregnant individuals (r = 0.48, P < 0.001). One hundred and eight miRNAs were differentially expressed between the normal and overweight/obese groups, which target genes were enriched in the metabolic pathway. Five C19MC miRNAs (miR-516a-5p, miR-516b-5p, miR-520a-3p, miR-1323, miR-523-5p) were upregulated in the overweight/obese group. Target enrichment analysis suggests their involvement in post-embryonic development, lipid and glucose homeostasis, T cell differentiation and nervous system development. Conclusions C19MC miRNA expression upregulation in the fetal circulation during the gestation of overweight/obese pregnant women may contribute to altered multisystem metabolic pathway development in their offspring. |
| first_indexed | 2024-12-13T05:37:11Z |
| format | Article |
| id | doaj.art-8362fd73a21e4506b4b80eae39b28896 |
| institution | Directory Open Access Journal |
| issn | 1743-7075 |
| language | English |
| last_indexed | 2024-12-13T05:37:11Z |
| publishDate | 2020-07-01 |
| publisher | BMC |
| record_format | Article |
| series | Nutrition & Metabolism |
| spelling | doaj.art-8362fd73a21e4506b4b80eae39b288962022-12-21T23:57:53ZengBMCNutrition & Metabolism1743-70752020-07-0117111010.1186/s12986-020-00475-7Maternal obesity alters C19MC microRNAs expression profile in fetal umbilical cord bloodJia Jing0Yingjin Wang1Yanmei Quan2Zhijie Wang3Yue Liu4Zhide Ding5Department of Histology, Embryology, Genetics and Developmental Biology, Shanghai Key Laboratory for Reproductive Medicine, Shanghai Jiao Tong University School of MedicineDepartment of Obstetrics and Gynecology, Shanghai Eighth People’s HospitalDepartment of Histology, Embryology, Genetics and Developmental Biology, Shanghai Key Laboratory for Reproductive Medicine, Shanghai Jiao Tong University School of MedicineDepartment of Obstetrics and Gynecology, Shanghai Eighth People’s HospitalDepartment of Histology, Embryology, Genetics and Developmental Biology, Shanghai Key Laboratory for Reproductive Medicine, Shanghai Jiao Tong University School of MedicineDepartment of Histology, Embryology, Genetics and Developmental Biology, Shanghai Key Laboratory for Reproductive Medicine, Shanghai Jiao Tong University School of MedicineAbstract Background To determine if overweight/obese pregnant women have altered microRNA expression patterns in fetal umbilical cord blood that may affect the development of offspring. Methods Umbilical cord blood samples were obtained from the fetuses of 34 overweight/obese and 32 normal-weight women after delivery. Next generation sequencing (NGS) analyzed their miRNA expression patterns. miRanda and TargetScan databases were used to predict the miRNAs’ target genes followed by analyses of Gene ontology (GO) and Kyoto Encyclopedia of Genes and Genomes (KEGG) to perform function grouping and pathway analyses. qRT-PCR verified the identity of differentially expressed miRNAs that were revealed in the NGS results. Results There was a positive correlation between newborn body weight and pregestational BMI of pregnant individuals (r = 0.48, P < 0.001). One hundred and eight miRNAs were differentially expressed between the normal and overweight/obese groups, which target genes were enriched in the metabolic pathway. Five C19MC miRNAs (miR-516a-5p, miR-516b-5p, miR-520a-3p, miR-1323, miR-523-5p) were upregulated in the overweight/obese group. Target enrichment analysis suggests their involvement in post-embryonic development, lipid and glucose homeostasis, T cell differentiation and nervous system development. Conclusions C19MC miRNA expression upregulation in the fetal circulation during the gestation of overweight/obese pregnant women may contribute to altered multisystem metabolic pathway development in their offspring.http://link.springer.com/article/10.1186/s12986-020-00475-7microRNAsObesityEpigeneticsUmbilical cord bloodFetus |
| spellingShingle | Jia Jing Yingjin Wang Yanmei Quan Zhijie Wang Yue Liu Zhide Ding Maternal obesity alters C19MC microRNAs expression profile in fetal umbilical cord blood Nutrition & Metabolism microRNAs Obesity Epigenetics Umbilical cord blood Fetus |
| title | Maternal obesity alters C19MC microRNAs expression profile in fetal umbilical cord blood |
| title_full | Maternal obesity alters C19MC microRNAs expression profile in fetal umbilical cord blood |
| title_fullStr | Maternal obesity alters C19MC microRNAs expression profile in fetal umbilical cord blood |
| title_full_unstemmed | Maternal obesity alters C19MC microRNAs expression profile in fetal umbilical cord blood |
| title_short | Maternal obesity alters C19MC microRNAs expression profile in fetal umbilical cord blood |
| title_sort | maternal obesity alters c19mc micrornas expression profile in fetal umbilical cord blood |
| topic | microRNAs Obesity Epigenetics Umbilical cord blood Fetus |
| url | http://link.springer.com/article/10.1186/s12986-020-00475-7 |
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