Ginsenoside Rb1 Mitigates <i>Escherichia coli</i> Lipopolysaccharide-Induced Endometritis through TLR4-Mediated NF-κB Pathway
Endometritis is the inflammatory response of the endometrial lining of the uterus and is associated with low conception rates, early embryonic mortality, and prolonged inter-calving intervals, and thus poses huge economic losses to the dairy industry worldwide. Ginsenoside Rb1 (GnRb1) is a natural c...
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| Format: | Article |
| Language: | English |
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MDPI AG
2021-11-01
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| Series: | Molecules |
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| Online Access: | https://www.mdpi.com/1420-3049/26/23/7089 |
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| author | Aftab Shaukat Irfan Shaukat Shahid Ali Rajput Rizwan Shukat Sana Hanif Imran Shaukat Xinxin Zhang Chao Chen Xuyang Sun Tingzhu Ye Kaifeng Niu Zhiqiu Yao Shadab Shaukat Muhammad Safdar Mohamed Abdelrahman Umair Riaz Junwei Zhao Xiaoying Gu Liguo Yang |
| author_facet | Aftab Shaukat Irfan Shaukat Shahid Ali Rajput Rizwan Shukat Sana Hanif Imran Shaukat Xinxin Zhang Chao Chen Xuyang Sun Tingzhu Ye Kaifeng Niu Zhiqiu Yao Shadab Shaukat Muhammad Safdar Mohamed Abdelrahman Umair Riaz Junwei Zhao Xiaoying Gu Liguo Yang |
| author_sort | Aftab Shaukat |
| collection | DOAJ |
| description | Endometritis is the inflammatory response of the endometrial lining of the uterus and is associated with low conception rates, early embryonic mortality, and prolonged inter-calving intervals, and thus poses huge economic losses to the dairy industry worldwide. Ginsenoside Rb1 (GnRb1) is a natural compound obtained from the roots of <i>Panax ginseng</i>, having several pharmacological and biological properties. However, the anti-inflammatory properties of GnRb1 in lipopolysaccharide (LPS)-challenged endometritis through the TLR4-mediated NF-κB signaling pathway has not yet been researched. This study was planned to evaluate the mechanisms of how GnRb1 rescues LPS-induced endometritis. In the present research, histopathological findings revealed that GnRb1 ameliorated LPS-triggered uterine injury. The ELISA and RT-qPCR assay findings indicated that GnRb1 suppressed the expression level of pro-inflammatory molecules (TNF-α, IL-1β and IL-6) and boosted the level of anti-inflammatory (IL-10) cytokine. Furthermore, the molecular study suggested that GnRb1 attenuated TLR4-mediated NF-κB signaling. The results demonstrated the therapeutic efficacy of GnRb1 in the mouse model of LPS-triggered endometritis via the inhibition of the TLR4-associated NF-κB pathway. Taken together, this study provides a baseline for the protective effect of GnRb1 to treat endometritis in both humans and animals. |
| first_indexed | 2024-03-10T04:48:27Z |
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| id | doaj.art-83636be9a20442e48fff8e9eb19257cb |
| institution | Directory Open Access Journal |
| issn | 1420-3049 |
| language | English |
| last_indexed | 2024-03-10T04:48:27Z |
| publishDate | 2021-11-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Molecules |
| spelling | doaj.art-83636be9a20442e48fff8e9eb19257cb2023-11-23T02:47:10ZengMDPI AGMolecules1420-30492021-11-012623708910.3390/molecules26237089Ginsenoside Rb1 Mitigates <i>Escherichia coli</i> Lipopolysaccharide-Induced Endometritis through TLR4-Mediated NF-κB PathwayAftab Shaukat0Irfan Shaukat1Shahid Ali Rajput2Rizwan Shukat3Sana Hanif4Imran Shaukat5Xinxin Zhang6Chao Chen7Xuyang Sun8Tingzhu Ye9Kaifeng Niu10Zhiqiu Yao11Shadab Shaukat12Muhammad Safdar13Mohamed Abdelrahman14Umair Riaz15Junwei Zhao16Xiaoying Gu17Liguo Yang18National Center for International Research on Animal Genetics, Breeding and Reproduction (NCIRAGBR), Huazhong Agricultural University, Wuhan 430070, ChinaFaculty of Medicine, University of Lorraine, 54052 Nancy, FranceFaculty of Veterinary and Animal Sciences, Muhammad Nawaz Shareef University of Agriculture, Multan 66000, PakistanFaculty of Food, Nutrition & Home Sciences, University of Agriculture, Faisalabad 38000, PakistanHubei Key Laboratory of Theory and Application of Advanced Materials Mechanics, Wuhan University of Technology, Wuhan 430070, ChinaDepartment of Physics, University of Agriculture, Faisalabad 38000, PakistanNational Center for International Research on Animal Genetics, Breeding and Reproduction (NCIRAGBR), Huazhong Agricultural University, Wuhan 430070, ChinaNational Center for International Research on Animal Genetics, Breeding and Reproduction (NCIRAGBR), Huazhong Agricultural University, Wuhan 430070, ChinaNational Center for International Research on Animal Genetics, Breeding and Reproduction (NCIRAGBR), Huazhong Agricultural University, Wuhan 430070, ChinaNational Center for International Research on Animal Genetics, Breeding and Reproduction (NCIRAGBR), Huazhong Agricultural University, Wuhan 430070, ChinaNational Center for International Research on Animal Genetics, Breeding and Reproduction (NCIRAGBR), Huazhong Agricultural University, Wuhan 430070, ChinaNational Center for International Research on Animal Genetics, Breeding and Reproduction (NCIRAGBR), Huazhong Agricultural University, Wuhan 430070, ChinaDepartment of Plant Breeding and Genetics, University of Agriculture, Faisalabad 38000, PakistanNational Center for International Research on Animal Genetics, Breeding and Reproduction (NCIRAGBR), Huazhong Agricultural University, Wuhan 430070, ChinaNational Center for International Research on Animal Genetics, Breeding and Reproduction (NCIRAGBR), Huazhong Agricultural University, Wuhan 430070, ChinaNational Center for International Research on Animal Genetics, Breeding and Reproduction (NCIRAGBR), Huazhong Agricultural University, Wuhan 430070, ChinaNational Center for International Research on Animal Genetics, Breeding and Reproduction (NCIRAGBR), Huazhong Agricultural University, Wuhan 430070, ChinaNational Center for International Research on Animal Genetics, Breeding and Reproduction (NCIRAGBR), Huazhong Agricultural University, Wuhan 430070, ChinaNational Center for International Research on Animal Genetics, Breeding and Reproduction (NCIRAGBR), Huazhong Agricultural University, Wuhan 430070, ChinaEndometritis is the inflammatory response of the endometrial lining of the uterus and is associated with low conception rates, early embryonic mortality, and prolonged inter-calving intervals, and thus poses huge economic losses to the dairy industry worldwide. Ginsenoside Rb1 (GnRb1) is a natural compound obtained from the roots of <i>Panax ginseng</i>, having several pharmacological and biological properties. However, the anti-inflammatory properties of GnRb1 in lipopolysaccharide (LPS)-challenged endometritis through the TLR4-mediated NF-κB signaling pathway has not yet been researched. This study was planned to evaluate the mechanisms of how GnRb1 rescues LPS-induced endometritis. In the present research, histopathological findings revealed that GnRb1 ameliorated LPS-triggered uterine injury. The ELISA and RT-qPCR assay findings indicated that GnRb1 suppressed the expression level of pro-inflammatory molecules (TNF-α, IL-1β and IL-6) and boosted the level of anti-inflammatory (IL-10) cytokine. Furthermore, the molecular study suggested that GnRb1 attenuated TLR4-mediated NF-κB signaling. The results demonstrated the therapeutic efficacy of GnRb1 in the mouse model of LPS-triggered endometritis via the inhibition of the TLR4-associated NF-κB pathway. Taken together, this study provides a baseline for the protective effect of GnRb1 to treat endometritis in both humans and animals.https://www.mdpi.com/1420-3049/26/23/7089endometritislipopolysaccharideGinsenoside Rb1TLR4NF-κB pathway |
| spellingShingle | Aftab Shaukat Irfan Shaukat Shahid Ali Rajput Rizwan Shukat Sana Hanif Imran Shaukat Xinxin Zhang Chao Chen Xuyang Sun Tingzhu Ye Kaifeng Niu Zhiqiu Yao Shadab Shaukat Muhammad Safdar Mohamed Abdelrahman Umair Riaz Junwei Zhao Xiaoying Gu Liguo Yang Ginsenoside Rb1 Mitigates <i>Escherichia coli</i> Lipopolysaccharide-Induced Endometritis through TLR4-Mediated NF-κB Pathway Molecules endometritis lipopolysaccharide Ginsenoside Rb1 TLR4 NF-κB pathway |
| title | Ginsenoside Rb1 Mitigates <i>Escherichia coli</i> Lipopolysaccharide-Induced Endometritis through TLR4-Mediated NF-κB Pathway |
| title_full | Ginsenoside Rb1 Mitigates <i>Escherichia coli</i> Lipopolysaccharide-Induced Endometritis through TLR4-Mediated NF-κB Pathway |
| title_fullStr | Ginsenoside Rb1 Mitigates <i>Escherichia coli</i> Lipopolysaccharide-Induced Endometritis through TLR4-Mediated NF-κB Pathway |
| title_full_unstemmed | Ginsenoside Rb1 Mitigates <i>Escherichia coli</i> Lipopolysaccharide-Induced Endometritis through TLR4-Mediated NF-κB Pathway |
| title_short | Ginsenoside Rb1 Mitigates <i>Escherichia coli</i> Lipopolysaccharide-Induced Endometritis through TLR4-Mediated NF-κB Pathway |
| title_sort | ginsenoside rb1 mitigates i escherichia coli i lipopolysaccharide induced endometritis through tlr4 mediated nf κb pathway |
| topic | endometritis lipopolysaccharide Ginsenoside Rb1 TLR4 NF-κB pathway |
| url | https://www.mdpi.com/1420-3049/26/23/7089 |
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