Histochemical Characterisation and Gene Expression Analysis of Skeletal Muscles from Maremmana and Aubrac Steers Reared on Grazing and Feedlot Systems
This study aimed to characterise the fibre composition of <i>Triceps brachii</i> (TB) and <i>Semimembranosus</i> (SM) muscles from 20 Maremmana (MA) and 20 Aubrac (AU) steers, and the effect of grazing activity in comparison with feedlot system. The histochemical method was p...
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MDPI AG
2021-03-01
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| Online Access: | https://www.mdpi.com/2076-2615/11/3/656 |
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| author | Giulia Foggi Francesca Ciucci Maria Conte Laura Casarosa Andrea Serra Elisabetta Giannessi Carla Lenzi Stefano Salvioli Giuseppe Conte Marcello Mele |
| author_facet | Giulia Foggi Francesca Ciucci Maria Conte Laura Casarosa Andrea Serra Elisabetta Giannessi Carla Lenzi Stefano Salvioli Giuseppe Conte Marcello Mele |
| author_sort | Giulia Foggi |
| collection | DOAJ |
| description | This study aimed to characterise the fibre composition of <i>Triceps brachii</i> (TB) and <i>Semimembranosus</i> (SM) muscles from 20 Maremmana (MA) and 20 Aubrac (AU) steers, and the effect of grazing activity in comparison with feedlot system. The histochemical method was performed with the m-ATPase method with an acid pre-incubation, thus allowing to distinguish type I, IIA, and IIB fibres. Additionally, on total RNA extracted from SM muscle, the expressions of atp1a1, mt-atp6, and capn1 genes were evaluated, in order to find potential associations with muscle fibre histochemical characteristics. In SM muscle, the MA steers had the greater frequency of oxidative fibres (type I and IIA) and the higher atp1a1 expression, in comparison to AU steers. Conversely, AU steers had a greater frequency of type IIB fibres, and the higher capn1 expression. A similar histochemical pattern was observed in TB muscle. The grazing activity was probably insufficient to determine differences both for fibre proportion and size, and gene expressions, except for mt-atp6 expression that was surprisingly highest in feedlot MA in comparison to other steers. These findings further the knowledge of muscle properties belonging to these breeds, and the effect of voluntary physical activity since few studies were available in this regard. |
| first_indexed | 2024-03-09T06:03:26Z |
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| id | doaj.art-8364a60847124ac891a6d87020f41917 |
| institution | Directory Open Access Journal |
| issn | 2076-2615 |
| language | English |
| last_indexed | 2024-03-09T06:03:26Z |
| publishDate | 2021-03-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Animals |
| spelling | doaj.art-8364a60847124ac891a6d87020f419172023-12-03T12:07:17ZengMDPI AGAnimals2076-26152021-03-0111365610.3390/ani11030656Histochemical Characterisation and Gene Expression Analysis of Skeletal Muscles from Maremmana and Aubrac Steers Reared on Grazing and Feedlot SystemsGiulia Foggi0Francesca Ciucci1Maria Conte2Laura Casarosa3Andrea Serra4Elisabetta Giannessi5Carla Lenzi6Stefano Salvioli7Giuseppe Conte8Marcello Mele9Dipartimento di Scienze Agrarie, Alimentari e Agro-ambientali, University of Pisa, 56124 Pisa, ItalyDipartimento di Scienze Agrarie, Alimentari e Agro-ambientali, University of Pisa, 56124 Pisa, ItalyDipartimento di Medicina Specialistica, Diagnostica e Sperimentale, University of Bologna, 40126 Bologna, ItalyDipartimento di Scienze Agrarie, Alimentari e Agro-ambientali, University of Pisa, 56124 Pisa, ItalyDipartimento di Scienze Agrarie, Alimentari e Agro-ambientali, University of Pisa, 56124 Pisa, ItalyDipartimento di Scienze Veterinarie, University of Pisa, 56124 Pisa, ItalyDipartimento di Scienze Veterinarie, University of Pisa, 56124 Pisa, ItalyDipartimento di Medicina Specialistica, Diagnostica e Sperimentale, University of Bologna, 40126 Bologna, ItalyDipartimento di Scienze Agrarie, Alimentari e Agro-ambientali, University of Pisa, 56124 Pisa, ItalyDipartimento di Scienze Agrarie, Alimentari e Agro-ambientali, University of Pisa, 56124 Pisa, ItalyThis study aimed to characterise the fibre composition of <i>Triceps brachii</i> (TB) and <i>Semimembranosus</i> (SM) muscles from 20 Maremmana (MA) and 20 Aubrac (AU) steers, and the effect of grazing activity in comparison with feedlot system. The histochemical method was performed with the m-ATPase method with an acid pre-incubation, thus allowing to distinguish type I, IIA, and IIB fibres. Additionally, on total RNA extracted from SM muscle, the expressions of atp1a1, mt-atp6, and capn1 genes were evaluated, in order to find potential associations with muscle fibre histochemical characteristics. In SM muscle, the MA steers had the greater frequency of oxidative fibres (type I and IIA) and the higher atp1a1 expression, in comparison to AU steers. Conversely, AU steers had a greater frequency of type IIB fibres, and the higher capn1 expression. A similar histochemical pattern was observed in TB muscle. The grazing activity was probably insufficient to determine differences both for fibre proportion and size, and gene expressions, except for mt-atp6 expression that was surprisingly highest in feedlot MA in comparison to other steers. These findings further the knowledge of muscle properties belonging to these breeds, and the effect of voluntary physical activity since few studies were available in this regard.https://www.mdpi.com/2076-2615/11/3/656rustic cattle breedgrazing activityatp1a1mt-atp6capn1 genesskeletal fibres frequency |
| spellingShingle | Giulia Foggi Francesca Ciucci Maria Conte Laura Casarosa Andrea Serra Elisabetta Giannessi Carla Lenzi Stefano Salvioli Giuseppe Conte Marcello Mele Histochemical Characterisation and Gene Expression Analysis of Skeletal Muscles from Maremmana and Aubrac Steers Reared on Grazing and Feedlot Systems Animals rustic cattle breed grazing activity atp1a1 mt-atp6 capn1 genes skeletal fibres frequency |
| title | Histochemical Characterisation and Gene Expression Analysis of Skeletal Muscles from Maremmana and Aubrac Steers Reared on Grazing and Feedlot Systems |
| title_full | Histochemical Characterisation and Gene Expression Analysis of Skeletal Muscles from Maremmana and Aubrac Steers Reared on Grazing and Feedlot Systems |
| title_fullStr | Histochemical Characterisation and Gene Expression Analysis of Skeletal Muscles from Maremmana and Aubrac Steers Reared on Grazing and Feedlot Systems |
| title_full_unstemmed | Histochemical Characterisation and Gene Expression Analysis of Skeletal Muscles from Maremmana and Aubrac Steers Reared on Grazing and Feedlot Systems |
| title_short | Histochemical Characterisation and Gene Expression Analysis of Skeletal Muscles from Maremmana and Aubrac Steers Reared on Grazing and Feedlot Systems |
| title_sort | histochemical characterisation and gene expression analysis of skeletal muscles from maremmana and aubrac steers reared on grazing and feedlot systems |
| topic | rustic cattle breed grazing activity atp1a1 mt-atp6 capn1 genes skeletal fibres frequency |
| url | https://www.mdpi.com/2076-2615/11/3/656 |
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