Studies on the Phytochemical Profile of <i>Ocimum basilicum</i> var. <i>minimum</i> (L.) Alef. Essential Oil, Its Larvicidal Activity and In Silico Interaction with Acetylcholinesterase against <i>Aedes aegypti</i> (Diptera: Culicidae)
<i>Aedes aegypti</i> L. (Diptera: Culicidae) is an important transmitter of diseases in tropical countries and controlling the larvae of this mosquito helps to reduce cases of diseases such as dengue, zika and chikungunya. Thus, the present study aimed to evaluate the larvicidal potentia...
Main Authors: | , , , , , , , , , |
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Format: | Article |
Language: | English |
Published: |
MDPI AG
2022-09-01
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Series: | International Journal of Molecular Sciences |
Subjects: | |
Online Access: | https://www.mdpi.com/1422-0067/23/19/11172 |
Summary: | <i>Aedes aegypti</i> L. (Diptera: Culicidae) is an important transmitter of diseases in tropical countries and controlling the larvae of this mosquito helps to reduce cases of diseases such as dengue, zika and chikungunya. Thus, the present study aimed to evaluate the larvicidal potential of the essential oil (EO) of <i>Ocimum basilicum</i> var. <i>minimum</i> (L.) Alef. The EO was extracted by stem distillation and the chemical composition was characterized by gas chromatography coupled with mass spectrometry (GC/MS and GC-FID). The larvicidal activity of EO was evaluated against third instar <i>Ae. aegypti</i> following World Health Organization (WHO) standard protocol and the interaction of the major compounds with the acetylcholinesterase (AChE) was evaluated by molecular docking. The predominant class was oxygenated monoterpenes with a concentration of 81.69% and the major compounds were limonene (9.5%), 1,8-cineole (14.23%), linalool (24.51%) and methyl chavicol (37.41%). The <i>O. basilicum</i> var. <i>minimum</i> EO showed unprecedented activity against third instar <i>Ae. aegypti</i> larvae at a dose-dependent relationship with LC<sub>50</sub> of 69.91 (µg/mL) and LC<sub>90</sub> of 200.62 (µg/mL), and the major compounds were able to interact with AChE in the Molecular Docking assay, indicating an ecological alternative for mosquito larvae control. |
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ISSN: | 1661-6596 1422-0067 |