Studies on the Phytochemical Profile of <i>Ocimum basilicum</i> var. <i>minimum</i> (L.) Alef. Essential Oil, Its Larvicidal Activity and In Silico Interaction with Acetylcholinesterase against <i>Aedes aegypti</i> (Diptera: Culicidae)
<i>Aedes aegypti</i> L. (Diptera: Culicidae) is an important transmitter of diseases in tropical countries and controlling the larvae of this mosquito helps to reduce cases of diseases such as dengue, zika and chikungunya. Thus, the present study aimed to evaluate the larvicidal potentia...
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| Format: | Article |
| Language: | English |
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MDPI AG
2022-09-01
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| Series: | International Journal of Molecular Sciences |
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| Online Access: | https://www.mdpi.com/1422-0067/23/19/11172 |
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| author | Anderson de Santana Botelho Oberdan Oliveira Ferreira Mozaniel Santana de Oliveira Jorddy Neves Cruz Sandro Henrique dos Reis Chaves Alejandro Ferraz do Prado Lidiane Diniz do Nascimento Geilson Alcantara da Silva Cristine Bastos do Amarante Eloisa Helena de Aguiar Andrade |
| author_facet | Anderson de Santana Botelho Oberdan Oliveira Ferreira Mozaniel Santana de Oliveira Jorddy Neves Cruz Sandro Henrique dos Reis Chaves Alejandro Ferraz do Prado Lidiane Diniz do Nascimento Geilson Alcantara da Silva Cristine Bastos do Amarante Eloisa Helena de Aguiar Andrade |
| author_sort | Anderson de Santana Botelho |
| collection | DOAJ |
| description | <i>Aedes aegypti</i> L. (Diptera: Culicidae) is an important transmitter of diseases in tropical countries and controlling the larvae of this mosquito helps to reduce cases of diseases such as dengue, zika and chikungunya. Thus, the present study aimed to evaluate the larvicidal potential of the essential oil (EO) of <i>Ocimum basilicum</i> var. <i>minimum</i> (L.) Alef. The EO was extracted by stem distillation and the chemical composition was characterized by gas chromatography coupled with mass spectrometry (GC/MS and GC-FID). The larvicidal activity of EO was evaluated against third instar <i>Ae. aegypti</i> following World Health Organization (WHO) standard protocol and the interaction of the major compounds with the acetylcholinesterase (AChE) was evaluated by molecular docking. The predominant class was oxygenated monoterpenes with a concentration of 81.69% and the major compounds were limonene (9.5%), 1,8-cineole (14.23%), linalool (24.51%) and methyl chavicol (37.41%). The <i>O. basilicum</i> var. <i>minimum</i> EO showed unprecedented activity against third instar <i>Ae. aegypti</i> larvae at a dose-dependent relationship with LC<sub>50</sub> of 69.91 (µg/mL) and LC<sub>90</sub> of 200.62 (µg/mL), and the major compounds were able to interact with AChE in the Molecular Docking assay, indicating an ecological alternative for mosquito larvae control. |
| first_indexed | 2024-03-09T21:42:15Z |
| format | Article |
| id | doaj.art-8365b62ebd4b4c63927dc22b62615612 |
| institution | Directory Open Access Journal |
| issn | 1661-6596 1422-0067 |
| language | English |
| last_indexed | 2024-03-09T21:42:15Z |
| publishDate | 2022-09-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | International Journal of Molecular Sciences |
| spelling | doaj.art-8365b62ebd4b4c63927dc22b626156122023-11-23T20:28:32ZengMDPI AGInternational Journal of Molecular Sciences1661-65961422-00672022-09-0123191117210.3390/ijms231911172Studies on the Phytochemical Profile of <i>Ocimum basilicum</i> var. <i>minimum</i> (L.) Alef. Essential Oil, Its Larvicidal Activity and In Silico Interaction with Acetylcholinesterase against <i>Aedes aegypti</i> (Diptera: Culicidae)Anderson de Santana Botelho0Oberdan Oliveira Ferreira1Mozaniel Santana de Oliveira2Jorddy Neves Cruz3Sandro Henrique dos Reis Chaves4Alejandro Ferraz do Prado5Lidiane Diniz do Nascimento6Geilson Alcantara da Silva7Cristine Bastos do Amarante8Eloisa Helena de Aguiar Andrade9Faculty of Chemistry, Institute of Exact and Natural Sciences, Federal University of Pará, Augusto Corrêa Street, S/N, Guamá, Belém 66075-900, Pará, BrazilAdolpho Ducke Laboratory—Botany Coordination, Emílio Goeldi Museum of Pará, Perimetral Avenue, 1901, Terra Firme, Belém 66077-830, Pará, BrazilAdolpho Ducke Laboratory—Botany Coordination, Emílio Goeldi Museum of Pará, Perimetral Avenue, 1901, Terra Firme, Belém 66077-830, Pará, BrazilLaboratory of Functional and Structural Biology, Institute of Biological Sciences, Federal University of Pará, Belém 66075-110, Pará, BrazilFaculty of Chemistry, Institute of Exact and Natural Sciences, Federal University of Pará, Augusto Corrêa Street, S/N, Guamá, Belém 66075-900, Pará, BrazilInstitute of Biological Sciences, Federal University of Pará, Augusto Corrêa Street, S/N, Guamá, Belém 66075-900, Pará, BrazilAdolpho Ducke Laboratory—Botany Coordination, Emílio Goeldi Museum of Pará, Perimetral Avenue, 1901, Terra Firme, Belém 66077-830, Pará, BrazilFaculty of Chemistry, Institute of Exact and Natural Sciences, Federal University of Pará, Augusto Corrêa Street, S/N, Guamá, Belém 66075-900, Pará, BrazilChemical Analysis Laboratory—Coordination of Earth Sciences and Ecology, Emílio Goeldi Museum of Pará, Perimetral Avenue, 1901, Terra Firme, Belém 66077-830, Pará, BrazilFaculty of Chemistry, Institute of Exact and Natural Sciences, Federal University of Pará, Augusto Corrêa Street, S/N, Guamá, Belém 66075-900, Pará, Brazil<i>Aedes aegypti</i> L. (Diptera: Culicidae) is an important transmitter of diseases in tropical countries and controlling the larvae of this mosquito helps to reduce cases of diseases such as dengue, zika and chikungunya. Thus, the present study aimed to evaluate the larvicidal potential of the essential oil (EO) of <i>Ocimum basilicum</i> var. <i>minimum</i> (L.) Alef. The EO was extracted by stem distillation and the chemical composition was characterized by gas chromatography coupled with mass spectrometry (GC/MS and GC-FID). The larvicidal activity of EO was evaluated against third instar <i>Ae. aegypti</i> following World Health Organization (WHO) standard protocol and the interaction of the major compounds with the acetylcholinesterase (AChE) was evaluated by molecular docking. The predominant class was oxygenated monoterpenes with a concentration of 81.69% and the major compounds were limonene (9.5%), 1,8-cineole (14.23%), linalool (24.51%) and methyl chavicol (37.41%). The <i>O. basilicum</i> var. <i>minimum</i> EO showed unprecedented activity against third instar <i>Ae. aegypti</i> larvae at a dose-dependent relationship with LC<sub>50</sub> of 69.91 (µg/mL) and LC<sub>90</sub> of 200.62 (µg/mL), and the major compounds were able to interact with AChE in the Molecular Docking assay, indicating an ecological alternative for mosquito larvae control.https://www.mdpi.com/1422-0067/23/19/11172natural products<i>Ocimum</i>larvaemosquitoacetylcholinesteraseecological |
| spellingShingle | Anderson de Santana Botelho Oberdan Oliveira Ferreira Mozaniel Santana de Oliveira Jorddy Neves Cruz Sandro Henrique dos Reis Chaves Alejandro Ferraz do Prado Lidiane Diniz do Nascimento Geilson Alcantara da Silva Cristine Bastos do Amarante Eloisa Helena de Aguiar Andrade Studies on the Phytochemical Profile of <i>Ocimum basilicum</i> var. <i>minimum</i> (L.) Alef. Essential Oil, Its Larvicidal Activity and In Silico Interaction with Acetylcholinesterase against <i>Aedes aegypti</i> (Diptera: Culicidae) International Journal of Molecular Sciences natural products <i>Ocimum</i> larvae mosquito acetylcholinesterase ecological |
| title | Studies on the Phytochemical Profile of <i>Ocimum basilicum</i> var. <i>minimum</i> (L.) Alef. Essential Oil, Its Larvicidal Activity and In Silico Interaction with Acetylcholinesterase against <i>Aedes aegypti</i> (Diptera: Culicidae) |
| title_full | Studies on the Phytochemical Profile of <i>Ocimum basilicum</i> var. <i>minimum</i> (L.) Alef. Essential Oil, Its Larvicidal Activity and In Silico Interaction with Acetylcholinesterase against <i>Aedes aegypti</i> (Diptera: Culicidae) |
| title_fullStr | Studies on the Phytochemical Profile of <i>Ocimum basilicum</i> var. <i>minimum</i> (L.) Alef. Essential Oil, Its Larvicidal Activity and In Silico Interaction with Acetylcholinesterase against <i>Aedes aegypti</i> (Diptera: Culicidae) |
| title_full_unstemmed | Studies on the Phytochemical Profile of <i>Ocimum basilicum</i> var. <i>minimum</i> (L.) Alef. Essential Oil, Its Larvicidal Activity and In Silico Interaction with Acetylcholinesterase against <i>Aedes aegypti</i> (Diptera: Culicidae) |
| title_short | Studies on the Phytochemical Profile of <i>Ocimum basilicum</i> var. <i>minimum</i> (L.) Alef. Essential Oil, Its Larvicidal Activity and In Silico Interaction with Acetylcholinesterase against <i>Aedes aegypti</i> (Diptera: Culicidae) |
| title_sort | studies on the phytochemical profile of i ocimum basilicum i var i minimum i l alef essential oil its larvicidal activity and in silico interaction with acetylcholinesterase against i aedes aegypti i diptera culicidae |
| topic | natural products <i>Ocimum</i> larvae mosquito acetylcholinesterase ecological |
| url | https://www.mdpi.com/1422-0067/23/19/11172 |
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