Progerin-Induced Impairment in Wound Healing and Proliferation in Vascular Endothelial Cells
Progerin as a mutated isoform of lamin A protein was first known to induce premature atherosclerosis progression in patients with Hutchinson-Gilford progeria syndrome (HGPS), and its role in provoking an inflammatory response in vascular cells and accelerating cell senescence has been investigated r...
| Main Authors: | , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
Frontiers Media S.A.
2022-03-01
|
| Series: | Frontiers in Aging |
| Subjects: | |
| Online Access: | https://www.frontiersin.org/articles/10.3389/fragi.2022.844885/full |
| _version_ | 1818137716459569152 |
|---|---|
| author | Yizhi Jiang Julie Y. Ji |
| author_facet | Yizhi Jiang Julie Y. Ji |
| author_sort | Yizhi Jiang |
| collection | DOAJ |
| description | Progerin as a mutated isoform of lamin A protein was first known to induce premature atherosclerosis progression in patients with Hutchinson-Gilford progeria syndrome (HGPS), and its role in provoking an inflammatory response in vascular cells and accelerating cell senescence has been investigated recently. However, how progerin triggers endothelial dysfunction that often occurs at the early stage of atherosclerosis in a mechanical environment has not been studied intensively. Here, we generated a stable endothelial cell line that expressed progerin and examined its effects on endothelial wound repair under laminar flow. We found decreased wound healing rate in progerin-expressing ECs under higher shear stress compared with those under low shear. Furthermore, the decreased wound recovery could be due to reduced number of cells at late mitosis, suggesting potential interference by progerin with endothelial proliferation. These findings provided insights into how progerin affects endothelial mechanotransduction and may contribute to the disruption of endothelial integrity in HGPS vasculature, as we continue to examine the mechanistic effect of progerin in shear-induced endothelial functions. |
| first_indexed | 2024-12-11T10:00:43Z |
| format | Article |
| id | doaj.art-83684321b093451e99a2674bc77cf74d |
| institution | Directory Open Access Journal |
| issn | 2673-6217 |
| language | English |
| last_indexed | 2024-12-11T10:00:43Z |
| publishDate | 2022-03-01 |
| publisher | Frontiers Media S.A. |
| record_format | Article |
| series | Frontiers in Aging |
| spelling | doaj.art-83684321b093451e99a2674bc77cf74d2022-12-22T01:12:08ZengFrontiers Media S.A.Frontiers in Aging2673-62172022-03-01310.3389/fragi.2022.844885844885Progerin-Induced Impairment in Wound Healing and Proliferation in Vascular Endothelial CellsYizhi JiangJulie Y. JiProgerin as a mutated isoform of lamin A protein was first known to induce premature atherosclerosis progression in patients with Hutchinson-Gilford progeria syndrome (HGPS), and its role in provoking an inflammatory response in vascular cells and accelerating cell senescence has been investigated recently. However, how progerin triggers endothelial dysfunction that often occurs at the early stage of atherosclerosis in a mechanical environment has not been studied intensively. Here, we generated a stable endothelial cell line that expressed progerin and examined its effects on endothelial wound repair under laminar flow. We found decreased wound healing rate in progerin-expressing ECs under higher shear stress compared with those under low shear. Furthermore, the decreased wound recovery could be due to reduced number of cells at late mitosis, suggesting potential interference by progerin with endothelial proliferation. These findings provided insights into how progerin affects endothelial mechanotransduction and may contribute to the disruption of endothelial integrity in HGPS vasculature, as we continue to examine the mechanistic effect of progerin in shear-induced endothelial functions.https://www.frontiersin.org/articles/10.3389/fragi.2022.844885/fullprogerinwound healingshear stressproliferationendothelial cellsvascular |
| spellingShingle | Yizhi Jiang Julie Y. Ji Progerin-Induced Impairment in Wound Healing and Proliferation in Vascular Endothelial Cells Frontiers in Aging progerin wound healing shear stress proliferation endothelial cells vascular |
| title | Progerin-Induced Impairment in Wound Healing and Proliferation in Vascular Endothelial Cells |
| title_full | Progerin-Induced Impairment in Wound Healing and Proliferation in Vascular Endothelial Cells |
| title_fullStr | Progerin-Induced Impairment in Wound Healing and Proliferation in Vascular Endothelial Cells |
| title_full_unstemmed | Progerin-Induced Impairment in Wound Healing and Proliferation in Vascular Endothelial Cells |
| title_short | Progerin-Induced Impairment in Wound Healing and Proliferation in Vascular Endothelial Cells |
| title_sort | progerin induced impairment in wound healing and proliferation in vascular endothelial cells |
| topic | progerin wound healing shear stress proliferation endothelial cells vascular |
| url | https://www.frontiersin.org/articles/10.3389/fragi.2022.844885/full |
| work_keys_str_mv | AT yizhijiang progerininducedimpairmentinwoundhealingandproliferationinvascularendothelialcells AT julieyji progerininducedimpairmentinwoundhealingandproliferationinvascularendothelialcells |