Polyhydroxyalkanoate Nanoparticles for Pulmonary Drug Delivery: Interaction with Lung Surfactant
Polyhydroxyalkanoates (PHA) are polyesters produced intracellularly by many bacterial species as energy storage materials, which are used in biomedical applications, including drug delivery systems, due to their biocompatibility and biodegradability. In this study, we evaluated the potential applica...
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| Format: | Article |
| Language: | English |
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MDPI AG
2021-06-01
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| Series: | Nanomaterials |
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| Online Access: | https://www.mdpi.com/2079-4991/11/6/1482 |
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| author | Olga Cañadas Andrea García-García M. Auxiliadora Prieto Jesús Pérez-Gil |
| author_facet | Olga Cañadas Andrea García-García M. Auxiliadora Prieto Jesús Pérez-Gil |
| author_sort | Olga Cañadas |
| collection | DOAJ |
| description | Polyhydroxyalkanoates (PHA) are polyesters produced intracellularly by many bacterial species as energy storage materials, which are used in biomedical applications, including drug delivery systems, due to their biocompatibility and biodegradability. In this study, we evaluated the potential application of this nanomaterial as a basis of inhaled drug delivery systems. To that end, we assessed the possible interaction between PHA nanoparticles (NPs) and pulmonary surfactant using dynamic light scattering, Langmuir balances, and epifluorescence microscopy. Our results demonstrate that NPs deposited onto preformed monolayers of DPPC or DPPC/POPG bind these surfactant lipids. This interaction facilitated the translocation of the nanomaterial towards the aqueous subphase, with the subsequent loss of lipid from the interface. NPs that remained at the interface associated with liquid expanded (LE)/tilted condensed (TC) phase boundaries, decreasing the size of condensed domains and promoting the intermixing of TC and LE phases at submicroscopic scale. This provided the stability necessary for attaining high surface pressures upon compression, countering the destabilization induced by lipid loss. These effects were observed only for high NP loads, suggesting a limit for the use of these NPs in pulmonary drug delivery. |
| first_indexed | 2024-03-10T10:44:58Z |
| format | Article |
| id | doaj.art-836a358828274dfa93bb6121b6f9844d |
| institution | Directory Open Access Journal |
| issn | 2079-4991 |
| language | English |
| last_indexed | 2024-03-10T10:44:58Z |
| publishDate | 2021-06-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Nanomaterials |
| spelling | doaj.art-836a358828274dfa93bb6121b6f9844d2023-11-21T22:38:55ZengMDPI AGNanomaterials2079-49912021-06-01116148210.3390/nano11061482Polyhydroxyalkanoate Nanoparticles for Pulmonary Drug Delivery: Interaction with Lung SurfactantOlga Cañadas0Andrea García-García1M. Auxiliadora Prieto2Jesús Pérez-Gil3Department of Biochemistry and Molecular Biology, Faculty of Biology, Complutense University, 28040 Madrid, SpainDepartment of Biochemistry and Molecular Biology, Faculty of Biology, Complutense University, 28040 Madrid, SpainDepartment of Microbial and Plant Biotechnology, Centro de Investigaciones Biológicas Margarita Salas (CIB-CSIC), 28040 Madrid, SpainDepartment of Biochemistry and Molecular Biology, Faculty of Biology, Complutense University, 28040 Madrid, SpainPolyhydroxyalkanoates (PHA) are polyesters produced intracellularly by many bacterial species as energy storage materials, which are used in biomedical applications, including drug delivery systems, due to their biocompatibility and biodegradability. In this study, we evaluated the potential application of this nanomaterial as a basis of inhaled drug delivery systems. To that end, we assessed the possible interaction between PHA nanoparticles (NPs) and pulmonary surfactant using dynamic light scattering, Langmuir balances, and epifluorescence microscopy. Our results demonstrate that NPs deposited onto preformed monolayers of DPPC or DPPC/POPG bind these surfactant lipids. This interaction facilitated the translocation of the nanomaterial towards the aqueous subphase, with the subsequent loss of lipid from the interface. NPs that remained at the interface associated with liquid expanded (LE)/tilted condensed (TC) phase boundaries, decreasing the size of condensed domains and promoting the intermixing of TC and LE phases at submicroscopic scale. This provided the stability necessary for attaining high surface pressures upon compression, countering the destabilization induced by lipid loss. These effects were observed only for high NP loads, suggesting a limit for the use of these NPs in pulmonary drug delivery.https://www.mdpi.com/2079-4991/11/6/1482DPPCPOPGPHAdynamic light scatteringlipid monolayersrelaxation kinetics |
| spellingShingle | Olga Cañadas Andrea García-García M. Auxiliadora Prieto Jesús Pérez-Gil Polyhydroxyalkanoate Nanoparticles for Pulmonary Drug Delivery: Interaction with Lung Surfactant Nanomaterials DPPC POPG PHA dynamic light scattering lipid monolayers relaxation kinetics |
| title | Polyhydroxyalkanoate Nanoparticles for Pulmonary Drug Delivery: Interaction with Lung Surfactant |
| title_full | Polyhydroxyalkanoate Nanoparticles for Pulmonary Drug Delivery: Interaction with Lung Surfactant |
| title_fullStr | Polyhydroxyalkanoate Nanoparticles for Pulmonary Drug Delivery: Interaction with Lung Surfactant |
| title_full_unstemmed | Polyhydroxyalkanoate Nanoparticles for Pulmonary Drug Delivery: Interaction with Lung Surfactant |
| title_short | Polyhydroxyalkanoate Nanoparticles for Pulmonary Drug Delivery: Interaction with Lung Surfactant |
| title_sort | polyhydroxyalkanoate nanoparticles for pulmonary drug delivery interaction with lung surfactant |
| topic | DPPC POPG PHA dynamic light scattering lipid monolayers relaxation kinetics |
| url | https://www.mdpi.com/2079-4991/11/6/1482 |
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