Can Spesific Biomarkers Be Used to Enlighten the Major Mechanisms of Acute High Dose Diclofenac Sodium-Related Nephrotoxicity?
Aim:The aim of this study was to examine the basic mechanisms that play a role in the acute nephrotoxicity caused by diclofenac sodium.Materials and Methods:Only water was given to the control group; however, the diclofenac sodium group was group intoxicated by giving water-soluble, 240 mg/kg, oral...
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| Format: | Article |
| Language: | English |
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Galenos Yayinevi
2022-06-01
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| Series: | Eurasian Journal of Emergency Medicine |
| Subjects: | |
| Online Access: |
http://eajem.com/archives/archive-detail/article-preview/can-spesific-biomarkers-be-used-to-enlighten-the-m/52307
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| author | Sinem Doğruyol İlker Akbaş Abdullah Osman Koçak Serpil Aygörmez Habip Emrah Leylek Sultan Tuna Akgöl Gür Özge Ertener |
| author_facet | Sinem Doğruyol İlker Akbaş Abdullah Osman Koçak Serpil Aygörmez Habip Emrah Leylek Sultan Tuna Akgöl Gür Özge Ertener |
| author_sort | Sinem Doğruyol |
| collection | DOAJ |
| description | Aim:The aim of this study was to examine the basic mechanisms that play a role in the acute nephrotoxicity caused by diclofenac sodium.Materials and Methods:Only water was given to the control group; however, the diclofenac sodium group was group intoxicated by giving water-soluble, 240 mg/kg, oral single dose diclofenac sodium. After 24 hours, all animals were sacrificed and histopathological analyzes were performed. The levels of spesific biomarkers [vascular endothelial growth factor (VEGF), nuclear factor-kappa B (NF-κB), matrix metalloproteinase-9 (MMP-9), metalloproteinase tissue inhibitor-1 (TIMP-1) and carcinoembryonic antigen (CEA)] that may be related to the nephrotoxicity mechanism were evaluated.Results:As a result of biochemical analysis, we found that VEGF, TIMP-1, NF-κB and CEA levels were significantly higher and MMP-9 levels were significantly lower in diclofenac sodium group compared to control group. Nephrotoxicity related histopathological changes were observed in the sections of diclofenac sodium group.Conclusion:This study has shown that the biomarkers we evaluated in the diclofenac sodium-induced acute high-dose intoxication model we created can help us to identify the nephrotoxicity and to explain the nephrotoxicity mechanism with the three main steps (the hemodynamicrelated pathway, the inflammation-related pathway, and the oxidative stress-related pathway). With a simple version of this panel adapted to emergency departments, we may be able to diagnose diclofenac sodium-related nephrotoxicity. |
| first_indexed | 2024-04-10T14:42:41Z |
| format | Article |
| id | doaj.art-836a502029894509beea8133ba552602 |
| institution | Directory Open Access Journal |
| issn | 2149-5807 2149-6048 |
| language | English |
| last_indexed | 2024-04-10T14:42:41Z |
| publishDate | 2022-06-01 |
| publisher | Galenos Yayinevi |
| record_format | Article |
| series | Eurasian Journal of Emergency Medicine |
| spelling | doaj.art-836a502029894509beea8133ba5526022023-02-15T16:08:05ZengGalenos YayineviEurasian Journal of Emergency Medicine2149-58072149-60482022-06-0121213213710.4274/eajem.galenos.2021.4546713049054Can Spesific Biomarkers Be Used to Enlighten the Major Mechanisms of Acute High Dose Diclofenac Sodium-Related Nephrotoxicity?Sinem Doğruyol0İlker Akbaş1Abdullah Osman Koçak2Serpil Aygörmez3Habip Emrah Leylek4Sultan Tuna Akgöl Gür5Özge Ertener6 Department of Emergency Medicine, Manisa Merkez Efendi State Hospital, Manisa, Turkey Department of Emergency Medicine, Bingöl State Hospital, Bingöl, Turkey Department of Emergency Medicine, Atatürk University Faculty of Medicine, Erzurum, Turkey Department of Biochemistry, University of Kafkas Faculty of Veterinary Medicine, Kars, Turkey Department of Emergency Medicine, Bandırma State Hospital, Balıkesir, Turkey Department of Emergency Medicine, Atatürk University Faculty of Medicine, Erzurum, Turkey Department of Pathology, İzmir University of Economics Faculty of Medicine, İzmir, Turkey Aim:The aim of this study was to examine the basic mechanisms that play a role in the acute nephrotoxicity caused by diclofenac sodium.Materials and Methods:Only water was given to the control group; however, the diclofenac sodium group was group intoxicated by giving water-soluble, 240 mg/kg, oral single dose diclofenac sodium. After 24 hours, all animals were sacrificed and histopathological analyzes were performed. The levels of spesific biomarkers [vascular endothelial growth factor (VEGF), nuclear factor-kappa B (NF-κB), matrix metalloproteinase-9 (MMP-9), metalloproteinase tissue inhibitor-1 (TIMP-1) and carcinoembryonic antigen (CEA)] that may be related to the nephrotoxicity mechanism were evaluated.Results:As a result of biochemical analysis, we found that VEGF, TIMP-1, NF-κB and CEA levels were significantly higher and MMP-9 levels were significantly lower in diclofenac sodium group compared to control group. Nephrotoxicity related histopathological changes were observed in the sections of diclofenac sodium group.Conclusion:This study has shown that the biomarkers we evaluated in the diclofenac sodium-induced acute high-dose intoxication model we created can help us to identify the nephrotoxicity and to explain the nephrotoxicity mechanism with the three main steps (the hemodynamicrelated pathway, the inflammation-related pathway, and the oxidative stress-related pathway). With a simple version of this panel adapted to emergency departments, we may be able to diagnose diclofenac sodium-related nephrotoxicity. http://eajem.com/archives/archive-detail/article-preview/can-spesific-biomarkers-be-used-to-enlighten-the-m/52307 diclofenac sodiumintoxicationnephrotoxicity |
| spellingShingle | Sinem Doğruyol İlker Akbaş Abdullah Osman Koçak Serpil Aygörmez Habip Emrah Leylek Sultan Tuna Akgöl Gür Özge Ertener Can Spesific Biomarkers Be Used to Enlighten the Major Mechanisms of Acute High Dose Diclofenac Sodium-Related Nephrotoxicity? Eurasian Journal of Emergency Medicine diclofenac sodium intoxication nephrotoxicity |
| title | Can Spesific Biomarkers Be Used to Enlighten the Major Mechanisms of Acute High Dose Diclofenac Sodium-Related Nephrotoxicity? |
| title_full | Can Spesific Biomarkers Be Used to Enlighten the Major Mechanisms of Acute High Dose Diclofenac Sodium-Related Nephrotoxicity? |
| title_fullStr | Can Spesific Biomarkers Be Used to Enlighten the Major Mechanisms of Acute High Dose Diclofenac Sodium-Related Nephrotoxicity? |
| title_full_unstemmed | Can Spesific Biomarkers Be Used to Enlighten the Major Mechanisms of Acute High Dose Diclofenac Sodium-Related Nephrotoxicity? |
| title_short | Can Spesific Biomarkers Be Used to Enlighten the Major Mechanisms of Acute High Dose Diclofenac Sodium-Related Nephrotoxicity? |
| title_sort | can spesific biomarkers be used to enlighten the major mechanisms of acute high dose diclofenac sodium related nephrotoxicity |
| topic | diclofenac sodium intoxication nephrotoxicity |
| url |
http://eajem.com/archives/archive-detail/article-preview/can-spesific-biomarkers-be-used-to-enlighten-the-m/52307
|
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