Identification of the Abundant Hydroxyproline-Rich Glycoproteins in the Root Walls of Wild-Type Arabidopsis, an ext3 Mutant Line, and Its Phenotypic Revertant
Extensins are members of the cell wall hydroxyproline-rich glycoprotein (HRGP) superfamily that form covalently cross-linked networks in primary cell walls. A knockout mutation in EXT3 (AT1G21310), the gene coding EXTENSIN 3 (EXT3) in Arabidopsis Landsberg erecta resulted in a lethal phenotype, alth...
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MDPI AG
2015-01-01
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| Series: | Plants |
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| Online Access: | http://www.mdpi.com/2223-7747/4/1/85 |
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| author | Yuning Chen Dening Ye Michael A. Held Maura C. Cannon Tui Ray Prasenjit Saha Alexandra N. Frye Andrew J. Mort Marcia J. Kieliszewski |
| author_facet | Yuning Chen Dening Ye Michael A. Held Maura C. Cannon Tui Ray Prasenjit Saha Alexandra N. Frye Andrew J. Mort Marcia J. Kieliszewski |
| author_sort | Yuning Chen |
| collection | DOAJ |
| description | Extensins are members of the cell wall hydroxyproline-rich glycoprotein (HRGP) superfamily that form covalently cross-linked networks in primary cell walls. A knockout mutation in EXT3 (AT1G21310), the gene coding EXTENSIN 3 (EXT3) in Arabidopsis Landsberg erecta resulted in a lethal phenotype, although about 20% of the knockout plants have an apparently normal phenotype (ANP). In this study the root cell wall HRGP components of wild-type, ANP and the ext3 mutant seedlings were characterized by peptide fractionation of trypsin digested anhydrous hydrogen fluoride deglycosylated wall residues and by sequencing using LC-MS/MS. Several HRGPs, including EXT3, were identified in the wild-type root walls but not in walls of the ANP and lethal mutant. Indeed the ANP walls and walls of mutants displaying the lethal phenotype possessed HRGPs, but the profiles suggest that changes in the amount and perhaps type may account for the corresponding phenotypes. |
| first_indexed | 2024-12-20T23:13:26Z |
| format | Article |
| id | doaj.art-83766ade5dd04896b9e7c7ef6f9603ec |
| institution | Directory Open Access Journal |
| issn | 2223-7747 |
| language | English |
| last_indexed | 2024-12-20T23:13:26Z |
| publishDate | 2015-01-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Plants |
| spelling | doaj.art-83766ade5dd04896b9e7c7ef6f9603ec2022-12-21T19:23:41ZengMDPI AGPlants2223-77472015-01-01418511110.3390/plants4010085plants4010085Identification of the Abundant Hydroxyproline-Rich Glycoproteins in the Root Walls of Wild-Type Arabidopsis, an ext3 Mutant Line, and Its Phenotypic RevertantYuning Chen0Dening Ye1Michael A. Held2Maura C. Cannon3Tui Ray4Prasenjit Saha5Alexandra N. Frye6Andrew J. Mort7Marcia J. Kieliszewski8Department of Chemistry and Biochemistry, Ohio University, Athens, OH 45701, USADepartment of Chemistry and Biochemistry, Ohio University, Athens, OH 45701, USADepartment of Chemistry and Biochemistry, Ohio University, Athens, OH 45701, USADepartment of Biochemistry and Molecular Biology, University of Massachusetts, Amherst, MA 01003, USADepartment of Biochemistry and Molecular Biology, University of Massachusetts, Amherst, MA 01003, USADepartment of Biochemistry and Molecular Biology, University of Massachusetts, Amherst, MA 01003, USADepartment of Chemistry and Biochemistry, Ohio University, Athens, OH 45701, USABiochemistry and Molecular Biology, Oklahoma State University, Stillwater, OK 74078, USADepartment of Chemistry and Biochemistry, Ohio University, Athens, OH 45701, USAExtensins are members of the cell wall hydroxyproline-rich glycoprotein (HRGP) superfamily that form covalently cross-linked networks in primary cell walls. A knockout mutation in EXT3 (AT1G21310), the gene coding EXTENSIN 3 (EXT3) in Arabidopsis Landsberg erecta resulted in a lethal phenotype, although about 20% of the knockout plants have an apparently normal phenotype (ANP). In this study the root cell wall HRGP components of wild-type, ANP and the ext3 mutant seedlings were characterized by peptide fractionation of trypsin digested anhydrous hydrogen fluoride deglycosylated wall residues and by sequencing using LC-MS/MS. Several HRGPs, including EXT3, were identified in the wild-type root walls but not in walls of the ANP and lethal mutant. Indeed the ANP walls and walls of mutants displaying the lethal phenotype possessed HRGPs, but the profiles suggest that changes in the amount and perhaps type may account for the corresponding phenotypes.http://www.mdpi.com/2223-7747/4/1/85Arabidopsis rootcell wallhydroxyproline-rich glycoproteinExtensin 3LRXPRP4proteomics |
| spellingShingle | Yuning Chen Dening Ye Michael A. Held Maura C. Cannon Tui Ray Prasenjit Saha Alexandra N. Frye Andrew J. Mort Marcia J. Kieliszewski Identification of the Abundant Hydroxyproline-Rich Glycoproteins in the Root Walls of Wild-Type Arabidopsis, an ext3 Mutant Line, and Its Phenotypic Revertant Plants Arabidopsis root cell wall hydroxyproline-rich glycoprotein Extensin 3 LRX PRP4 proteomics |
| title | Identification of the Abundant Hydroxyproline-Rich Glycoproteins in the Root Walls of Wild-Type Arabidopsis, an ext3 Mutant Line, and Its Phenotypic Revertant |
| title_full | Identification of the Abundant Hydroxyproline-Rich Glycoproteins in the Root Walls of Wild-Type Arabidopsis, an ext3 Mutant Line, and Its Phenotypic Revertant |
| title_fullStr | Identification of the Abundant Hydroxyproline-Rich Glycoproteins in the Root Walls of Wild-Type Arabidopsis, an ext3 Mutant Line, and Its Phenotypic Revertant |
| title_full_unstemmed | Identification of the Abundant Hydroxyproline-Rich Glycoproteins in the Root Walls of Wild-Type Arabidopsis, an ext3 Mutant Line, and Its Phenotypic Revertant |
| title_short | Identification of the Abundant Hydroxyproline-Rich Glycoproteins in the Root Walls of Wild-Type Arabidopsis, an ext3 Mutant Line, and Its Phenotypic Revertant |
| title_sort | identification of the abundant hydroxyproline rich glycoproteins in the root walls of wild type arabidopsis an ext3 mutant line and its phenotypic revertant |
| topic | Arabidopsis root cell wall hydroxyproline-rich glycoprotein Extensin 3 LRX PRP4 proteomics |
| url | http://www.mdpi.com/2223-7747/4/1/85 |
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