MOLECULAR TYPING OF STREPTOCOCCUS PNEUMONIAE BY THE MULTIPLEX POLYMERASE CHAIN REACTION ASSAY IN ACCORDANCE TO THE PREVALENCE OF SEROTYPES IN THE RUSSIAN FEDERATION
Aim: to compare two methods of S. pneumoniae molecular typing: classic serological method and the multiplex polymerase chain reaction (M-PCR) assay modified in accordance to the date on the serotypes circulating in Russian Federation. Patients and methods: 420 pneumococcal isolates mainly from non-s...
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| Language: | English |
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"Paediatrician" Publishers LLC
2013-12-01
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| Series: | Вопросы современной педиатрии |
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| Online Access: | https://vsp.spr-journal.ru/jour/article/view/253 |
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| author | N. M. Alyab'eva T. A. Blinova O. A. Ponomarenko A. V. Lazareva L. K. Katosova N. A. Mayanskii |
| author_facet | N. M. Alyab'eva T. A. Blinova O. A. Ponomarenko A. V. Lazareva L. K. Katosova N. A. Mayanskii |
| author_sort | N. M. Alyab'eva |
| collection | DOAJ |
| description | Aim: to compare two methods of S. pneumoniae molecular typing: classic serological method and the multiplex polymerase chain reaction (M-PCR) assay modified in accordance to the date on the serotypes circulating in Russian Federation. Patients and methods: 420 pneumococcal isolates mainly from non-sterile loci were analyzed. After microbiological identification pneumococci were serologically typed with the means of specific antiserum produced by Staten Serum Institute (Denmark) in latex agglutination test and/or capsular swelling method. At the same time we performed series of the M-PCR, which consisted of 7 consecutive reactions at the most. Results: serotype was identified by the means of serological method in all 420 strains of S. pneumoniae; in total we determined 24 different serotypes. By the means of the M-PCR we succeeded in identification of 95% (399/420) examined strains, and 90% of them were typed during the first 3 reactions. All isolates failed to be typed by M-PCR (n =21) have serotypes not included into the composition of the M-PCR. The results of serological and molecular typing were identical in 99,2% (396/399) of the isolates; 3 strains showed contradictory results: serotype 19A was found on serological assay and serotype 19F — on PCR assay. Conclusions: the introduced modification of M-PCR allows correct identification of pneumococcal serotype more than in 90% of strains circulating in the Russian Federation, including all serotypes of pneumococcal polysaccharide conjugate vaccine. |
| first_indexed | 2024-03-12T04:22:05Z |
| format | Article |
| id | doaj.art-83780adf4a684af48a6a12dd1456d6e7 |
| institution | Directory Open Access Journal |
| issn | 1682-5527 1682-5535 |
| language | English |
| last_indexed | 2024-03-12T04:22:05Z |
| publishDate | 2013-12-01 |
| publisher | "Paediatrician" Publishers LLC |
| record_format | Article |
| series | Вопросы современной педиатрии |
| spelling | doaj.art-83780adf4a684af48a6a12dd1456d6e72023-09-03T10:33:01Zeng"Paediatrician" Publishers LLCВопросы современной педиатрии1682-55271682-55352013-12-01126303410.15690/vsp.v12i6.871253MOLECULAR TYPING OF STREPTOCOCCUS PNEUMONIAE BY THE MULTIPLEX POLYMERASE CHAIN REACTION ASSAY IN ACCORDANCE TO THE PREVALENCE OF SEROTYPES IN THE RUSSIAN FEDERATIONN. M. Alyab'eva0T. A. Blinova1O. A. Ponomarenko2A. V. Lazareva3L. K. Katosova4N. A. Mayanskii5Научный центр здоровья детей РАМН, МоскваНаучный центр здоровья детей РАМН, МоскваНаучный центр здоровья детей РАМН, МоскваНаучный центр здоровья детей РАМН, МоскваНаучный центр здоровья детей РАМН, МоскваНаучный центр здоровья детей РАМН, Москва; Первый Московский государственный медицинский университет им. И.М. СеченоваAim: to compare two methods of S. pneumoniae molecular typing: classic serological method and the multiplex polymerase chain reaction (M-PCR) assay modified in accordance to the date on the serotypes circulating in Russian Federation. Patients and methods: 420 pneumococcal isolates mainly from non-sterile loci were analyzed. After microbiological identification pneumococci were serologically typed with the means of specific antiserum produced by Staten Serum Institute (Denmark) in latex agglutination test and/or capsular swelling method. At the same time we performed series of the M-PCR, which consisted of 7 consecutive reactions at the most. Results: serotype was identified by the means of serological method in all 420 strains of S. pneumoniae; in total we determined 24 different serotypes. By the means of the M-PCR we succeeded in identification of 95% (399/420) examined strains, and 90% of them were typed during the first 3 reactions. All isolates failed to be typed by M-PCR (n =21) have serotypes not included into the composition of the M-PCR. The results of serological and molecular typing were identical in 99,2% (396/399) of the isolates; 3 strains showed contradictory results: serotype 19A was found on serological assay and serotype 19F — on PCR assay. Conclusions: the introduced modification of M-PCR allows correct identification of pneumococcal serotype more than in 90% of strains circulating in the Russian Federation, including all serotypes of pneumococcal polysaccharide conjugate vaccine.https://vsp.spr-journal.ru/jour/article/view/253streptococcus pneumoniaeсеротипмультиплексная пцр |
| spellingShingle | N. M. Alyab'eva T. A. Blinova O. A. Ponomarenko A. V. Lazareva L. K. Katosova N. A. Mayanskii MOLECULAR TYPING OF STREPTOCOCCUS PNEUMONIAE BY THE MULTIPLEX POLYMERASE CHAIN REACTION ASSAY IN ACCORDANCE TO THE PREVALENCE OF SEROTYPES IN THE RUSSIAN FEDERATION Вопросы современной педиатрии streptococcus pneumoniae серотип мультиплексная пцр |
| title | MOLECULAR TYPING OF STREPTOCOCCUS PNEUMONIAE BY THE MULTIPLEX POLYMERASE CHAIN REACTION ASSAY IN ACCORDANCE TO THE PREVALENCE OF SEROTYPES IN THE RUSSIAN FEDERATION |
| title_full | MOLECULAR TYPING OF STREPTOCOCCUS PNEUMONIAE BY THE MULTIPLEX POLYMERASE CHAIN REACTION ASSAY IN ACCORDANCE TO THE PREVALENCE OF SEROTYPES IN THE RUSSIAN FEDERATION |
| title_fullStr | MOLECULAR TYPING OF STREPTOCOCCUS PNEUMONIAE BY THE MULTIPLEX POLYMERASE CHAIN REACTION ASSAY IN ACCORDANCE TO THE PREVALENCE OF SEROTYPES IN THE RUSSIAN FEDERATION |
| title_full_unstemmed | MOLECULAR TYPING OF STREPTOCOCCUS PNEUMONIAE BY THE MULTIPLEX POLYMERASE CHAIN REACTION ASSAY IN ACCORDANCE TO THE PREVALENCE OF SEROTYPES IN THE RUSSIAN FEDERATION |
| title_short | MOLECULAR TYPING OF STREPTOCOCCUS PNEUMONIAE BY THE MULTIPLEX POLYMERASE CHAIN REACTION ASSAY IN ACCORDANCE TO THE PREVALENCE OF SEROTYPES IN THE RUSSIAN FEDERATION |
| title_sort | molecular typing of streptococcus pneumoniae by the multiplex polymerase chain reaction assay in accordance to the prevalence of serotypes in the russian federation |
| topic | streptococcus pneumoniae серотип мультиплексная пцр |
| url | https://vsp.spr-journal.ru/jour/article/view/253 |
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