Plant Synthetic Promoters: Advancement and Prospective
Native/endogenous promoters have several fundamental limitations in terms of their size, <i>Cis-</i>elements distribution/patterning, and mode of induction, which is ultimately reflected in their insufficient transcriptional activity. Several customized synthetic promoters were designed...
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| Format: | Article |
| Language: | English |
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MDPI AG
2023-01-01
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| Series: | Agriculture |
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| Online Access: | https://www.mdpi.com/2077-0472/13/2/298 |
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| author | Ahamed Khan Noohi Nasim Baveesh Pudhuvai Bhupendra Koul Santosh Kumar Upadhyay Lini Sethi Nrisingha Dey |
| author_facet | Ahamed Khan Noohi Nasim Baveesh Pudhuvai Bhupendra Koul Santosh Kumar Upadhyay Lini Sethi Nrisingha Dey |
| author_sort | Ahamed Khan |
| collection | DOAJ |
| description | Native/endogenous promoters have several fundamental limitations in terms of their size, <i>Cis-</i>elements distribution/patterning, and mode of induction, which is ultimately reflected in their insufficient transcriptional activity. Several customized synthetic promoters were designed and tested in plants during the past decade to circumvent such constraints. Such synthetic promoters have a built-in capacity to drive the expression of the foreign genes at their maximum amplitude in plant orthologous systems. The basic structure and function of the promoter has been discussed in this review, with emphasis on the role of the <i>Cis</i>-element in regulating gene expression. In addition to this, the necessity of synthetic promoters in the arena of plant biology has been highlighted. This review also provides explicit information on the two major approaches for developing plant-based synthetic promoters: the conventional approach (by utilizing the basic knowledge of promoter structure and <i>Cis</i>-trans interaction) and the advancement in gene editing technology. The success of plant genetic manipulation relies on the promoter efficiency and the expression level of the transgene. Therefore, advancements in the field of synthetic promoters has enormous potential in genetic engineering-mediated crop improvement. |
| first_indexed | 2024-03-11T09:19:02Z |
| format | Article |
| id | doaj.art-8379119ed52a450f8dc9d80d1d59c3e0 |
| institution | Directory Open Access Journal |
| issn | 2077-0472 |
| language | English |
| last_indexed | 2024-03-11T09:19:02Z |
| publishDate | 2023-01-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Agriculture |
| spelling | doaj.art-8379119ed52a450f8dc9d80d1d59c3e02023-11-16T18:29:06ZengMDPI AGAgriculture2077-04722023-01-0113229810.3390/agriculture13020298Plant Synthetic Promoters: Advancement and ProspectiveAhamed Khan0Noohi Nasim1Baveesh Pudhuvai2Bhupendra Koul3Santosh Kumar Upadhyay4Lini Sethi5Nrisingha Dey6Biology Centre of the Czech Academy of Sciences, Institute of Plant Molecular Biology, 370 05 České Budějovice, Czech RepublicDivision of Microbial and Plant Biotechnology, Institute of Life Sciences, Department of Biotechnology, Government of India, Bhubaneswar 751023, Odisha, IndiaDepartment of Genetics and Biotechnology, Faculty of Agriculture and Technology, University of South Bohemia in České Budějovice, 370 05 České Budějovice, Czech RepublicDepartment of Biotechnology, Lovely Professional University, Phagwara 144411, Punjab, IndiaDepartment of Botany, Panjab University, Chandigarh 160014, Punjab, IndiaDivision of Microbial and Plant Biotechnology, Institute of Life Sciences, Department of Biotechnology, Government of India, Bhubaneswar 751023, Odisha, IndiaDivision of Microbial and Plant Biotechnology, Institute of Life Sciences, Department of Biotechnology, Government of India, Bhubaneswar 751023, Odisha, IndiaNative/endogenous promoters have several fundamental limitations in terms of their size, <i>Cis-</i>elements distribution/patterning, and mode of induction, which is ultimately reflected in their insufficient transcriptional activity. Several customized synthetic promoters were designed and tested in plants during the past decade to circumvent such constraints. Such synthetic promoters have a built-in capacity to drive the expression of the foreign genes at their maximum amplitude in plant orthologous systems. The basic structure and function of the promoter has been discussed in this review, with emphasis on the role of the <i>Cis</i>-element in regulating gene expression. In addition to this, the necessity of synthetic promoters in the arena of plant biology has been highlighted. This review also provides explicit information on the two major approaches for developing plant-based synthetic promoters: the conventional approach (by utilizing the basic knowledge of promoter structure and <i>Cis</i>-trans interaction) and the advancement in gene editing technology. The success of plant genetic manipulation relies on the promoter efficiency and the expression level of the transgene. Therefore, advancements in the field of synthetic promoters has enormous potential in genetic engineering-mediated crop improvement.https://www.mdpi.com/2077-0472/13/2/298synthetic promoterCRISPR<i>Cis</i>-engineeringtranscription factorsgene expression |
| spellingShingle | Ahamed Khan Noohi Nasim Baveesh Pudhuvai Bhupendra Koul Santosh Kumar Upadhyay Lini Sethi Nrisingha Dey Plant Synthetic Promoters: Advancement and Prospective Agriculture synthetic promoter CRISPR <i>Cis</i>-engineering transcription factors gene expression |
| title | Plant Synthetic Promoters: Advancement and Prospective |
| title_full | Plant Synthetic Promoters: Advancement and Prospective |
| title_fullStr | Plant Synthetic Promoters: Advancement and Prospective |
| title_full_unstemmed | Plant Synthetic Promoters: Advancement and Prospective |
| title_short | Plant Synthetic Promoters: Advancement and Prospective |
| title_sort | plant synthetic promoters advancement and prospective |
| topic | synthetic promoter CRISPR <i>Cis</i>-engineering transcription factors gene expression |
| url | https://www.mdpi.com/2077-0472/13/2/298 |
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