Apoptosis-like cell death upon kinetoplastid induction by compounds isolated from the brown algae Dictyota spiralis
Abstract Background The in vitro activity of the brown seaweed Dictyota spiralis against both Leishmania amazonensis and Trypanosoma cruzi was evaluated in a previous study. Processing by bio-guided fractionation resulted in the isolation of three active compounds, classified as diterpenes. In the p...
| Main Authors: | , , , , , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
BMC
2021-04-01
|
| Series: | Parasites & Vectors |
| Subjects: | |
| Online Access: | https://doi.org/10.1186/s13071-021-04693-7 |
| _version_ | 1818598209119846400 |
|---|---|
| author | Olfa Chiboub Ines Sifaoui Manef Abderrabba Mondher Mejri José J. Fernández Ana R. Díaz-Marrero Jacob Lorenzo-Morales José E. Piñero |
| author_facet | Olfa Chiboub Ines Sifaoui Manef Abderrabba Mondher Mejri José J. Fernández Ana R. Díaz-Marrero Jacob Lorenzo-Morales José E. Piñero |
| author_sort | Olfa Chiboub |
| collection | DOAJ |
| description | Abstract Background The in vitro activity of the brown seaweed Dictyota spiralis against both Leishmania amazonensis and Trypanosoma cruzi was evaluated in a previous study. Processing by bio-guided fractionation resulted in the isolation of three active compounds, classified as diterpenes. In the present study, we performed several assays to detect clinical features associated to cell death in L. amazonensis and T. cruzi with the aim to elucidate the mechanism of action of these compounds on parasitic cells. Methods The aims of the experiments were to detect and evaluate specific events involved in apoptosis-like cell death in the kinetoplastid, including DNA condensation, accumulation of reactive oxygen species and changes in ATP concentration, cell permeability and mitochondrial membrane potential, respectively, in treated cells. Results The results demonstrated that the three isolated diterpenes could inhibit the tested parasites by inducing an apoptosis-like cell death. Conclusions These results encourage further investigation on the isolated compounds as potential drug candidates against both L. amazonensis and T. cruzi. Graphic abstract |
| first_indexed | 2024-12-16T12:00:03Z |
| format | Article |
| id | doaj.art-837ca87966d642e8a7e6c99eaeb84760 |
| institution | Directory Open Access Journal |
| issn | 1756-3305 |
| language | English |
| last_indexed | 2024-12-16T12:00:03Z |
| publishDate | 2021-04-01 |
| publisher | BMC |
| record_format | Article |
| series | Parasites & Vectors |
| spelling | doaj.art-837ca87966d642e8a7e6c99eaeb847602022-12-21T22:32:28ZengBMCParasites & Vectors1756-33052021-04-0114111310.1186/s13071-021-04693-7Apoptosis-like cell death upon kinetoplastid induction by compounds isolated from the brown algae Dictyota spiralisOlfa Chiboub0Ines Sifaoui1Manef Abderrabba2Mondher Mejri3José J. Fernández4Ana R. Díaz-Marrero5Jacob Lorenzo-Morales6José E. Piñero7Instituto Universitario de Enfermedades Tropicales y Salud Pública de Canarias, Universidad de La Laguna (ULL)Instituto Universitario de Enfermedades Tropicales y Salud Pública de Canarias, Universidad de La Laguna (ULL)Laboratoire Matériaux-Molécules et Applications, University of CarthageLaboratoire Matériaux-Molécules et Applications, University of CarthageInstituto Universitario de Bio-Orgánica Antonio González, Universidad de La Laguna (ULL)Instituto Universitario de Bio-Orgánica Antonio González, Universidad de La Laguna (ULL)Instituto Universitario de Enfermedades Tropicales y Salud Pública de Canarias, Universidad de La Laguna (ULL)Instituto Universitario de Enfermedades Tropicales y Salud Pública de Canarias, Universidad de La Laguna (ULL)Abstract Background The in vitro activity of the brown seaweed Dictyota spiralis against both Leishmania amazonensis and Trypanosoma cruzi was evaluated in a previous study. Processing by bio-guided fractionation resulted in the isolation of three active compounds, classified as diterpenes. In the present study, we performed several assays to detect clinical features associated to cell death in L. amazonensis and T. cruzi with the aim to elucidate the mechanism of action of these compounds on parasitic cells. Methods The aims of the experiments were to detect and evaluate specific events involved in apoptosis-like cell death in the kinetoplastid, including DNA condensation, accumulation of reactive oxygen species and changes in ATP concentration, cell permeability and mitochondrial membrane potential, respectively, in treated cells. Results The results demonstrated that the three isolated diterpenes could inhibit the tested parasites by inducing an apoptosis-like cell death. Conclusions These results encourage further investigation on the isolated compounds as potential drug candidates against both L. amazonensis and T. cruzi. Graphic abstracthttps://doi.org/10.1186/s13071-021-04693-7T. cruziL. amazonensisDiterpenesMarine compoundsAntikinetoplastidApoptosis-like |
| spellingShingle | Olfa Chiboub Ines Sifaoui Manef Abderrabba Mondher Mejri José J. Fernández Ana R. Díaz-Marrero Jacob Lorenzo-Morales José E. Piñero Apoptosis-like cell death upon kinetoplastid induction by compounds isolated from the brown algae Dictyota spiralis Parasites & Vectors T. cruzi L. amazonensis Diterpenes Marine compounds Antikinetoplastid Apoptosis-like |
| title | Apoptosis-like cell death upon kinetoplastid induction by compounds isolated from the brown algae Dictyota spiralis |
| title_full | Apoptosis-like cell death upon kinetoplastid induction by compounds isolated from the brown algae Dictyota spiralis |
| title_fullStr | Apoptosis-like cell death upon kinetoplastid induction by compounds isolated from the brown algae Dictyota spiralis |
| title_full_unstemmed | Apoptosis-like cell death upon kinetoplastid induction by compounds isolated from the brown algae Dictyota spiralis |
| title_short | Apoptosis-like cell death upon kinetoplastid induction by compounds isolated from the brown algae Dictyota spiralis |
| title_sort | apoptosis like cell death upon kinetoplastid induction by compounds isolated from the brown algae dictyota spiralis |
| topic | T. cruzi L. amazonensis Diterpenes Marine compounds Antikinetoplastid Apoptosis-like |
| url | https://doi.org/10.1186/s13071-021-04693-7 |
| work_keys_str_mv | AT olfachiboub apoptosislikecelldeathuponkinetoplastidinductionbycompoundsisolatedfromthebrownalgaedictyotaspiralis AT inessifaoui apoptosislikecelldeathuponkinetoplastidinductionbycompoundsisolatedfromthebrownalgaedictyotaspiralis AT manefabderrabba apoptosislikecelldeathuponkinetoplastidinductionbycompoundsisolatedfromthebrownalgaedictyotaspiralis AT mondhermejri apoptosislikecelldeathuponkinetoplastidinductionbycompoundsisolatedfromthebrownalgaedictyotaspiralis AT josejfernandez apoptosislikecelldeathuponkinetoplastidinductionbycompoundsisolatedfromthebrownalgaedictyotaspiralis AT anardiazmarrero apoptosislikecelldeathuponkinetoplastidinductionbycompoundsisolatedfromthebrownalgaedictyotaspiralis AT jacoblorenzomorales apoptosislikecelldeathuponkinetoplastidinductionbycompoundsisolatedfromthebrownalgaedictyotaspiralis AT joseepinero apoptosislikecelldeathuponkinetoplastidinductionbycompoundsisolatedfromthebrownalgaedictyotaspiralis |