Ecotoxicological Studies of ZnO and CdS Nanoparticles on <i>Chlorella vulgaris</i> Photosynthetic Microorganism in Seine River Water
Seine river water was used as natural environmental medium to study the ecotoxicological impact of ZnO and CdS nanoparticles and Zn<sup>2+</sup> and Cd<sup>2+</sup> free ions using <i>Chlorella vulgaris</i> as a biological target. It was demonstrated by viability...
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MDPI AG
2020-01-01
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| Series: | Nanomaterials |
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| Online Access: | https://www.mdpi.com/2079-4991/10/2/227 |
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| author | Alice da Rocha Nicolas Menguy Claude Yéprémian Alain Couté Roberta Brayner |
| author_facet | Alice da Rocha Nicolas Menguy Claude Yéprémian Alain Couté Roberta Brayner |
| author_sort | Alice da Rocha |
| collection | DOAJ |
| description | Seine river water was used as natural environmental medium to study the ecotoxicological impact of ZnO and CdS nanoparticles and Zn<sup>2+</sup> and Cd<sup>2+</sup> free ions using <i>Chlorella vulgaris</i> as a biological target. It was demonstrated by viability tests and photosynthetic activity measurements that free Zn<sup>2+</sup> (<i>IC</i><sub>50</sub> = 2.7 × 10<sup>−4</sup> M) is less toxic than free Cd<sup>2+</sup> and ZnO nanoparticles (<i>IC</i><sub>50</sub> = 1.4 × 10<sup>−4</sup> M). In the case of cadmium species, free Cd<sup>2+</sup> (<i>IC</i><sub>50</sub> = 3.5 × 10<sup>−5</sup> M) was similar to CdS nanoparticles (CdS-1: <i>IC</i><sub>50</sub> = 1.9 × 10<sup>−5</sup> M and CdS-2: <i>IC</i><sub>50</sub> = 1.9 × 10<sup>−5</sup> M), as follows: CdS > Cd<sup>2+</sup> > ZnO > Zn<sup>2+</sup>. Adenosine-5’-triphosphate (ATP) assay and superoxide dismutase (SOD) enzymatic activity confirmed these results. Transmission electron microscopy (TEM), coupled with energy-dispersive X-ray spectroscopy (EDS), confirmed the internalization of CdS-1 nanoparticles after 48 h of contact with <i>Chlorella vulgaris</i> at 10<sup>−3</sup> M. With a higher concentration of nanoparticles (10<sup>−2</sup> M), ZnO and CdS-2 were also localized inside cells. |
| first_indexed | 2024-12-23T10:26:16Z |
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| issn | 2079-4991 |
| language | English |
| last_indexed | 2024-12-23T10:26:16Z |
| publishDate | 2020-01-01 |
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| series | Nanomaterials |
| spelling | doaj.art-83847e0e6c434b238c9f9dc891dd673f2022-12-21T17:50:33ZengMDPI AGNanomaterials2079-49912020-01-0110222710.3390/nano10020227nano10020227Ecotoxicological Studies of ZnO and CdS Nanoparticles on <i>Chlorella vulgaris</i> Photosynthetic Microorganism in Seine River WaterAlice da Rocha0Nicolas Menguy1Claude Yéprémian2Alain Couté3Roberta Brayner4Université de Paris, ITODYS, UMR 7086, CNRS, 15 rue Jean-Antoine de Baïf, F-75205 Paris CEDEX 13, FranceSorbonne Université, Institut de Minéralogie et de Physique des Milieux Condensés UMR CNRS 7590, Institut de Recherche pour le Développement 4, place Jussieu, 75005 Paris, FranceMuséum National d’Histoire Naturelle, UMR 7245 CNRS, USM 505, Département Régulations, Développement, et Diversité Moléculaire (RDDM), 12 rue Buffon, F-75005 Paris, FranceMuséum National d’Histoire Naturelle, UMR 7245 CNRS, USM 505, Département Régulations, Développement, et Diversité Moléculaire (RDDM), 12 rue Buffon, F-75005 Paris, FranceUniversité de Paris, ITODYS, UMR 7086, CNRS, 15 rue Jean-Antoine de Baïf, F-75205 Paris CEDEX 13, FranceSeine river water was used as natural environmental medium to study the ecotoxicological impact of ZnO and CdS nanoparticles and Zn<sup>2+</sup> and Cd<sup>2+</sup> free ions using <i>Chlorella vulgaris</i> as a biological target. It was demonstrated by viability tests and photosynthetic activity measurements that free Zn<sup>2+</sup> (<i>IC</i><sub>50</sub> = 2.7 × 10<sup>−4</sup> M) is less toxic than free Cd<sup>2+</sup> and ZnO nanoparticles (<i>IC</i><sub>50</sub> = 1.4 × 10<sup>−4</sup> M). In the case of cadmium species, free Cd<sup>2+</sup> (<i>IC</i><sub>50</sub> = 3.5 × 10<sup>−5</sup> M) was similar to CdS nanoparticles (CdS-1: <i>IC</i><sub>50</sub> = 1.9 × 10<sup>−5</sup> M and CdS-2: <i>IC</i><sub>50</sub> = 1.9 × 10<sup>−5</sup> M), as follows: CdS > Cd<sup>2+</sup> > ZnO > Zn<sup>2+</sup>. Adenosine-5’-triphosphate (ATP) assay and superoxide dismutase (SOD) enzymatic activity confirmed these results. Transmission electron microscopy (TEM), coupled with energy-dispersive X-ray spectroscopy (EDS), confirmed the internalization of CdS-1 nanoparticles after 48 h of contact with <i>Chlorella vulgaris</i> at 10<sup>−3</sup> M. With a higher concentration of nanoparticles (10<sup>−2</sup> M), ZnO and CdS-2 were also localized inside cells.https://www.mdpi.com/2079-4991/10/2/227polyol processcdsznoecotoxicology<i>chlorella vulgaris</i> |
| spellingShingle | Alice da Rocha Nicolas Menguy Claude Yéprémian Alain Couté Roberta Brayner Ecotoxicological Studies of ZnO and CdS Nanoparticles on <i>Chlorella vulgaris</i> Photosynthetic Microorganism in Seine River Water Nanomaterials polyol process cds zno ecotoxicology <i>chlorella vulgaris</i> |
| title | Ecotoxicological Studies of ZnO and CdS Nanoparticles on <i>Chlorella vulgaris</i> Photosynthetic Microorganism in Seine River Water |
| title_full | Ecotoxicological Studies of ZnO and CdS Nanoparticles on <i>Chlorella vulgaris</i> Photosynthetic Microorganism in Seine River Water |
| title_fullStr | Ecotoxicological Studies of ZnO and CdS Nanoparticles on <i>Chlorella vulgaris</i> Photosynthetic Microorganism in Seine River Water |
| title_full_unstemmed | Ecotoxicological Studies of ZnO and CdS Nanoparticles on <i>Chlorella vulgaris</i> Photosynthetic Microorganism in Seine River Water |
| title_short | Ecotoxicological Studies of ZnO and CdS Nanoparticles on <i>Chlorella vulgaris</i> Photosynthetic Microorganism in Seine River Water |
| title_sort | ecotoxicological studies of zno and cds nanoparticles on i chlorella vulgaris i photosynthetic microorganism in seine river water |
| topic | polyol process cds zno ecotoxicology <i>chlorella vulgaris</i> |
| url | https://www.mdpi.com/2079-4991/10/2/227 |
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