Validation of a straightforward high performance liquid chromatographic method for morphine quantitation
Abstract Background Morphine is illegal to use unless it is prescribed by a medical doctor. This compound is commonly abused in the form of illicit heroin. Methods An easy-to-use method for determining morphine in illicit heroin using high performance liquid chromatography-photodiode array detector...
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| Format: | Article |
| Language: | English |
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SpringerOpen
2017-07-01
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| Series: | Egyptian Journal of Forensic Sciences |
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| Online Access: | http://link.springer.com/article/10.1186/s41935-017-0003-0 |
| _version_ | 1811318490774110208 |
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| author | Kar-Weng Chan |
| author_facet | Kar-Weng Chan |
| author_sort | Kar-Weng Chan |
| collection | DOAJ |
| description | Abstract Background Morphine is illegal to use unless it is prescribed by a medical doctor. This compound is commonly abused in the form of illicit heroin. Methods An easy-to-use method for determining morphine in illicit heroin using high performance liquid chromatography-photodiode array detector (HPLC-PDA) was developed. With the aid of 50:50 acetonitrile:ammonium formate, this target compound traveling in a pentafluorophenyl (PFP) column was successfully separated from other compounds frequently present in the heroin matrix. Results The method was precise with an intra-day relative standard deviation (RSD) ≤ 0.8% and inter-day RSD < 5%. It was able to detect as low as 0.001 mg/mL morphine. The detector’s response was linear (R 2 > 0.999) and reliable for morphine quantitation from 0.005 up to 1 mg/mL. Through recovery studies, accuracy of the method was averagely estimated to be 89.54–101.91%. Conclusions This method saves time in terms of mobile phase preparation and cuts cost by excluding additional purchase of expensive chemicals. More importantly, it proves to be able to determine the target analyte with sufficient accuracy and precision. |
| first_indexed | 2024-04-13T12:27:04Z |
| format | Article |
| id | doaj.art-8386e0aa7a184147b2bff85a5c73996e |
| institution | Directory Open Access Journal |
| issn | 2090-5939 |
| language | English |
| last_indexed | 2024-04-13T12:27:04Z |
| publishDate | 2017-07-01 |
| publisher | SpringerOpen |
| record_format | Article |
| series | Egyptian Journal of Forensic Sciences |
| spelling | doaj.art-8386e0aa7a184147b2bff85a5c73996e2022-12-22T02:46:59ZengSpringerOpenEgyptian Journal of Forensic Sciences2090-59392017-07-01711610.1186/s41935-017-0003-0Validation of a straightforward high performance liquid chromatographic method for morphine quantitationKar-Weng Chan0Research and Instrumentation Unit, Department of Chemistry Malaysia, Ministry of Science, Technology and Innovation (MOSTI)Abstract Background Morphine is illegal to use unless it is prescribed by a medical doctor. This compound is commonly abused in the form of illicit heroin. Methods An easy-to-use method for determining morphine in illicit heroin using high performance liquid chromatography-photodiode array detector (HPLC-PDA) was developed. With the aid of 50:50 acetonitrile:ammonium formate, this target compound traveling in a pentafluorophenyl (PFP) column was successfully separated from other compounds frequently present in the heroin matrix. Results The method was precise with an intra-day relative standard deviation (RSD) ≤ 0.8% and inter-day RSD < 5%. It was able to detect as low as 0.001 mg/mL morphine. The detector’s response was linear (R 2 > 0.999) and reliable for morphine quantitation from 0.005 up to 1 mg/mL. Through recovery studies, accuracy of the method was averagely estimated to be 89.54–101.91%. Conclusions This method saves time in terms of mobile phase preparation and cuts cost by excluding additional purchase of expensive chemicals. More importantly, it proves to be able to determine the target analyte with sufficient accuracy and precision.http://link.springer.com/article/10.1186/s41935-017-0003-0MorphineIllicit HeroinHPLCQuantitative method |
| spellingShingle | Kar-Weng Chan Validation of a straightforward high performance liquid chromatographic method for morphine quantitation Egyptian Journal of Forensic Sciences Morphine Illicit Heroin HPLC Quantitative method |
| title | Validation of a straightforward high performance liquid chromatographic method for morphine quantitation |
| title_full | Validation of a straightforward high performance liquid chromatographic method for morphine quantitation |
| title_fullStr | Validation of a straightforward high performance liquid chromatographic method for morphine quantitation |
| title_full_unstemmed | Validation of a straightforward high performance liquid chromatographic method for morphine quantitation |
| title_short | Validation of a straightforward high performance liquid chromatographic method for morphine quantitation |
| title_sort | validation of a straightforward high performance liquid chromatographic method for morphine quantitation |
| topic | Morphine Illicit Heroin HPLC Quantitative method |
| url | http://link.springer.com/article/10.1186/s41935-017-0003-0 |
| work_keys_str_mv | AT karwengchan validationofastraightforwardhighperformanceliquidchromatographicmethodformorphinequantitation |