Environmental genomics of a municipal landfill soil
We investigated the toxicity of soil samples derived from a former municipal landfill site in the South of the Netherlands, where a bioremediation project is running aiming at reusing the site for recreation. Both an organic soil extract and the original soil sample was investigated using the ISO st...
| Main Authors: | , , , , , |
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| Format: | Article |
| Language: | English |
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Frontiers Media S.A.
2012-05-01
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| Series: | Frontiers in Genetics |
| Subjects: | |
| Online Access: | http://journal.frontiersin.org/Journal/10.3389/fgene.2012.00085/full |
| _version_ | 1819114541985824768 |
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| author | Dick eRoelofs Muriel ede Boer Valeria eAgamennone Pascale eBouchier Juliette eLegler Nico evan Straalen |
| author_facet | Dick eRoelofs Muriel ede Boer Valeria eAgamennone Pascale eBouchier Juliette eLegler Nico evan Straalen |
| author_sort | Dick eRoelofs |
| collection | DOAJ |
| description | We investigated the toxicity of soil samples derived from a former municipal landfill site in the South of the Netherlands, where a bioremediation project is running aiming at reusing the site for recreation. Both an organic soil extract and the original soil sample was investigated using the ISO standardised Folsomia soil ecotoxicological testing and gene expression analysis. The 28 day survival/reproduction test revealed that the ecologically more relevant original soil sample was more toxic than the organic soil extract. Microarray analysis showed that the more toxic soil samples induced gene regulatory changes in twice as less genes compared to the soil extract. Consequently gene regulatory changes were highly dependent on sample type, and were to a lesser extent caused by exposure level. An important biological process shared among the two sample types was the detoxification pathway for xenobiotics (biotransformation I, II and III) suggesting a link between compound type and observed adverse effects. Finally, we were able to retrieve a selected group of genes that show highly significant dose-dependent gene expression and thus were tightly linked with adverse effects on reproduction. Expression of four cytochrome P450 genes showed highest correlation values with reproduction, and maybe promising genetic markers for soil quality. However, a more elaborate set of environmental soil samples is needed to validate the correlation between gene expression induction and adverse phenotypic effects. |
| first_indexed | 2024-12-22T04:46:57Z |
| format | Article |
| id | doaj.art-83880c0bdef7447aaa9201d9ae717b4a |
| institution | Directory Open Access Journal |
| issn | 1664-8021 |
| language | English |
| last_indexed | 2024-12-22T04:46:57Z |
| publishDate | 2012-05-01 |
| publisher | Frontiers Media S.A. |
| record_format | Article |
| series | Frontiers in Genetics |
| spelling | doaj.art-83880c0bdef7447aaa9201d9ae717b4a2022-12-21T18:38:35ZengFrontiers Media S.A.Frontiers in Genetics1664-80212012-05-01310.3389/fgene.2012.0008523010Environmental genomics of a municipal landfill soilDick eRoelofs0Muriel ede Boer1Valeria eAgamennone2Pascale eBouchier3Juliette eLegler4Nico evan Straalen5VU UniversityVU UniversityVU UniversityVU UniversityVU UniversityVU UniversityWe investigated the toxicity of soil samples derived from a former municipal landfill site in the South of the Netherlands, where a bioremediation project is running aiming at reusing the site for recreation. Both an organic soil extract and the original soil sample was investigated using the ISO standardised Folsomia soil ecotoxicological testing and gene expression analysis. The 28 day survival/reproduction test revealed that the ecologically more relevant original soil sample was more toxic than the organic soil extract. Microarray analysis showed that the more toxic soil samples induced gene regulatory changes in twice as less genes compared to the soil extract. Consequently gene regulatory changes were highly dependent on sample type, and were to a lesser extent caused by exposure level. An important biological process shared among the two sample types was the detoxification pathway for xenobiotics (biotransformation I, II and III) suggesting a link between compound type and observed adverse effects. Finally, we were able to retrieve a selected group of genes that show highly significant dose-dependent gene expression and thus were tightly linked with adverse effects on reproduction. Expression of four cytochrome P450 genes showed highest correlation values with reproduction, and maybe promising genetic markers for soil quality. However, a more elaborate set of environmental soil samples is needed to validate the correlation between gene expression induction and adverse phenotypic effects.http://journal.frontiersin.org/Journal/10.3389/fgene.2012.00085/fullBiotransformationMicroarrayFolsomia candidacytochrome P450 |
| spellingShingle | Dick eRoelofs Muriel ede Boer Valeria eAgamennone Pascale eBouchier Juliette eLegler Nico evan Straalen Environmental genomics of a municipal landfill soil Frontiers in Genetics Biotransformation Microarray Folsomia candida cytochrome P450 |
| title | Environmental genomics of a municipal landfill soil |
| title_full | Environmental genomics of a municipal landfill soil |
| title_fullStr | Environmental genomics of a municipal landfill soil |
| title_full_unstemmed | Environmental genomics of a municipal landfill soil |
| title_short | Environmental genomics of a municipal landfill soil |
| title_sort | environmental genomics of a municipal landfill soil |
| topic | Biotransformation Microarray Folsomia candida cytochrome P450 |
| url | http://journal.frontiersin.org/Journal/10.3389/fgene.2012.00085/full |
| work_keys_str_mv | AT dickeroelofs environmentalgenomicsofamunicipallandfillsoil AT murieledeboer environmentalgenomicsofamunicipallandfillsoil AT valeriaeagamennone environmentalgenomicsofamunicipallandfillsoil AT pascaleebouchier environmentalgenomicsofamunicipallandfillsoil AT julietteelegler environmentalgenomicsofamunicipallandfillsoil AT nicoevanstraalen environmentalgenomicsofamunicipallandfillsoil |