Multidrug-resistant Salmonella spp. in fecal samples of pigs with suspected salmonellosis in Antioquia, Colombia, 2019–2021
Objectives. To determine the proportion of Salmonella enterica in fecal samples of live pigs with suspected salmonellosis analyzed at the diagnostic unit of the University of Antioquia, Colombia between 2019 and 2021, and examine the serotypes and antimicrobial resistance patterns. Methods. This was...
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| Format: | Article |
| Language: | English |
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Pan American Health Organization
2023-04-01
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| Series: | Revista Panamericana de Salud Pública |
| Subjects: | |
| Online Access: | https://iris.paho.org/handle/10665.2/57327 |
| _version_ | 1797846228946386944 |
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| author | Juana L. Vidal Viviana Clavijo Luis R. Castellanos Jeyashree Kathiresan Ajay M.V. Kumar Kedar Mehta Jenny J. Chaparro-Gutiérrez |
| author_facet | Juana L. Vidal Viviana Clavijo Luis R. Castellanos Jeyashree Kathiresan Ajay M.V. Kumar Kedar Mehta Jenny J. Chaparro-Gutiérrez |
| author_sort | Juana L. Vidal |
| collection | DOAJ |
| description | Objectives. To determine the proportion of Salmonella enterica in fecal samples of live pigs with suspected salmonellosis analyzed at the diagnostic unit of the University of Antioquia, Colombia between 2019 and 2021, and examine the serotypes and antimicrobial resistance patterns.
Methods. This was a laboratory-based cross-sectional study of routine data on fecal samples received from pig farms in all nine subregions of Antioquia state, Colombia. Salmonella spp. detection at the university is done using enrichment, selective culture, and polymerase chain reaction. Serotypes were identified using the Kauffmann–White scheme and isolates were tested for antimicrobial susceptibility using broth microdilution.
Results. Of 653 samples tested, 149 (23%) were positive for S. enterica. Nine serotypes were identified. The most common were Salmonella Typhimurium (56%) and its monophasic variant (35%). Resistance to ampicillin (70%) was most frequently observed, followed by ciprofloxacin (55%), and sulfamethoxazole–trimethoprim (52%). No isolates were resistant to amikacin and gentamicin. Multidrug resistance (resistance to ≥ 3 classes of antibiotics) was observed in 61 (44%) isolates. Multidrug resistance was highest in S. Typhimurium (57%) compared with the other serotypes. Serotype was associated with multidrug resistance (p = 0.01), but age of the pig and sub-region were not.
Conclusions. The proportion of Salmonella spp. and the associated high levels of multidrug resistance are of concern and may indicate irrational use of antimicrobials and poor management practices in pig production systems in the region. Strengthened surveillance is needed to monitor and improve farm management practices and the use of antimicrobials in farms in Colombia. |
| first_indexed | 2024-04-09T17:52:40Z |
| format | Article |
| id | doaj.art-838a3d17edad4b26a9413dd6a4433b4f |
| institution | Directory Open Access Journal |
| issn | 1020-4989 1680-5348 |
| language | English |
| last_indexed | 2024-04-09T17:52:40Z |
| publishDate | 2023-04-01 |
| publisher | Pan American Health Organization |
| record_format | Article |
| series | Revista Panamericana de Salud Pública |
| spelling | doaj.art-838a3d17edad4b26a9413dd6a4433b4f2023-04-15T16:48:25ZengPan American Health OrganizationRevista Panamericana de Salud Pública1020-49891680-53482023-04-01474611010.26633/RPSP.2023.46rpspMultidrug-resistant Salmonella spp. in fecal samples of pigs with suspected salmonellosis in Antioquia, Colombia, 2019–2021Juana L. Vidal0Viviana Clavijo1Luis R. Castellanos2Jeyashree Kathiresan3Ajay M.V. Kumar4Kedar Mehta5Jenny J. Chaparro-Gutiérrez6Faculty of Agrarian Sciences, University of Antioquia, Medellin, Colombia.Ciencia y tecnologia de Fagos – Sciphage, Bogota, Colombia.Quadram Institute Bioscience, Norwich, United Kingdom of Great Britain and Northern Ireland.Indian Council of Medical Research, National Institute of Epidemiology, Chennai, India.International Union Against Tuberculosis and Lung Disease, Paris, France.GMERS Medical College, Gotri, Vadodara, Gujarat, India.Faculty of Agrarian Sciences, University of Antioquia, Medellin, Colombia.Objectives. To determine the proportion of Salmonella enterica in fecal samples of live pigs with suspected salmonellosis analyzed at the diagnostic unit of the University of Antioquia, Colombia between 2019 and 2021, and examine the serotypes and antimicrobial resistance patterns. Methods. This was a laboratory-based cross-sectional study of routine data on fecal samples received from pig farms in all nine subregions of Antioquia state, Colombia. Salmonella spp. detection at the university is done using enrichment, selective culture, and polymerase chain reaction. Serotypes were identified using the Kauffmann–White scheme and isolates were tested for antimicrobial susceptibility using broth microdilution. Results. Of 653 samples tested, 149 (23%) were positive for S. enterica. Nine serotypes were identified. The most common were Salmonella Typhimurium (56%) and its monophasic variant (35%). Resistance to ampicillin (70%) was most frequently observed, followed by ciprofloxacin (55%), and sulfamethoxazole–trimethoprim (52%). No isolates were resistant to amikacin and gentamicin. Multidrug resistance (resistance to ≥ 3 classes of antibiotics) was observed in 61 (44%) isolates. Multidrug resistance was highest in S. Typhimurium (57%) compared with the other serotypes. Serotype was associated with multidrug resistance (p = 0.01), but age of the pig and sub-region were not. Conclusions. The proportion of Salmonella spp. and the associated high levels of multidrug resistance are of concern and may indicate irrational use of antimicrobials and poor management practices in pig production systems in the region. Strengthened surveillance is needed to monitor and improve farm management practices and the use of antimicrobials in farms in Colombia.https://iris.paho.org/handle/10665.2/57327salmonella entericaserogroupdrug resistance, multipleswinecolombia |
| spellingShingle | Juana L. Vidal Viviana Clavijo Luis R. Castellanos Jeyashree Kathiresan Ajay M.V. Kumar Kedar Mehta Jenny J. Chaparro-Gutiérrez Multidrug-resistant Salmonella spp. in fecal samples of pigs with suspected salmonellosis in Antioquia, Colombia, 2019–2021 Revista Panamericana de Salud Pública salmonella enterica serogroup drug resistance, multiple swine colombia |
| title | Multidrug-resistant Salmonella spp. in fecal samples of pigs with suspected salmonellosis in Antioquia, Colombia, 2019–2021 |
| title_full | Multidrug-resistant Salmonella spp. in fecal samples of pigs with suspected salmonellosis in Antioquia, Colombia, 2019–2021 |
| title_fullStr | Multidrug-resistant Salmonella spp. in fecal samples of pigs with suspected salmonellosis in Antioquia, Colombia, 2019–2021 |
| title_full_unstemmed | Multidrug-resistant Salmonella spp. in fecal samples of pigs with suspected salmonellosis in Antioquia, Colombia, 2019–2021 |
| title_short | Multidrug-resistant Salmonella spp. in fecal samples of pigs with suspected salmonellosis in Antioquia, Colombia, 2019–2021 |
| title_sort | multidrug resistant salmonella spp in fecal samples of pigs with suspected salmonellosis in antioquia colombia 2019 2021 |
| topic | salmonella enterica serogroup drug resistance, multiple swine colombia |
| url | https://iris.paho.org/handle/10665.2/57327 |
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