Changes in the Flower and Leaf Proteome of Common Buckwheat (<i>Fagopyrum esculentum</i> Moench) under High Temperature
Common buckwheat (<i>Fagopyrum esculentum</i> Moench), a pseudocereal crop, produces a large number of flowers, but this does not guarantee high seed yields. This species demonstrates strong abortion of flowers and embryos. High temperatures during the generative growth phase result in a...
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MDPI AG
2021-03-01
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| Series: | International Journal of Molecular Sciences |
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| Online Access: | https://www.mdpi.com/1422-0067/22/5/2678 |
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| author | Przemysław Kopeć Marta Hornyák Jakub Pastuszak Anna Szczerba Marcin Rapacz Jacek Waga Agnieszka Płażek |
| author_facet | Przemysław Kopeć Marta Hornyák Jakub Pastuszak Anna Szczerba Marcin Rapacz Jacek Waga Agnieszka Płażek |
| author_sort | Przemysław Kopeć |
| collection | DOAJ |
| description | Common buckwheat (<i>Fagopyrum esculentum</i> Moench), a pseudocereal crop, produces a large number of flowers, but this does not guarantee high seed yields. This species demonstrates strong abortion of flowers and embryos. High temperatures during the generative growth phase result in an increase in the degeneration of embryo sacs. The aim of this study was to investigate proteomic changes in flowers and leaves of two common buckwheat accessions with different degrees of heat tolerance, Panda and PA15. Two-dimensional gel electrophoresis and mass spectrometry techniques were used to analyze the proteome profiles. Analyses were conducted for flower buds, open flowers capable of fertilization, and wilted flowers, as well as donor leaves, i.e., those growing closest to the inflorescences. High temperature up-regulated the expression of 182 proteins. The proteomic response to heat stress differed between the accessions and among their organs. In the Panda accession, we observed a change in abundance of 17, 13, 28, and 11 proteins, in buds, open and wilted flowers, and leaves, respectively. However, in the PA15 accession there were 34, 21, 63, and 21 such proteins, respectively. Fifteen heat-affected proteins were common to both accessions. The indole-3-glycerol phosphate synthase chloroplastic-like isoform X2 accumulated in the open flowers of the heat-sensitive cultivar Panda in response to high temperature, and may be a candidate protein as a marker of heat sensitivity in buckwheat plants. |
| first_indexed | 2024-03-09T05:09:10Z |
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| id | doaj.art-838c802af7684ae2abce167661272e49 |
| institution | Directory Open Access Journal |
| issn | 1661-6596 1422-0067 |
| language | English |
| last_indexed | 2024-03-09T05:09:10Z |
| publishDate | 2021-03-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | International Journal of Molecular Sciences |
| spelling | doaj.art-838c802af7684ae2abce167661272e492023-12-03T12:51:34ZengMDPI AGInternational Journal of Molecular Sciences1661-65961422-00672021-03-01225267810.3390/ijms22052678Changes in the Flower and Leaf Proteome of Common Buckwheat (<i>Fagopyrum esculentum</i> Moench) under High TemperaturePrzemysław Kopeć0Marta Hornyák1Jakub Pastuszak2Anna Szczerba3Marcin Rapacz4Jacek Waga5Agnieszka Płażek6Polish Academy of Sciences, Institute of Plant Physiology, Niezapominajek 21, 30-239 Kraków, PolandDepartment of Plant Breeding, Physiology and Seed Science, University of Agriculture, Podłużna 3, 30-239 Kraków, PolandDepartment of Plant Breeding, Physiology and Seed Science, University of Agriculture, Podłużna 3, 30-239 Kraków, PolandDepartment of Plant Breeding, Physiology and Seed Science, University of Agriculture, Podłużna 3, 30-239 Kraków, PolandDepartment of Plant Breeding, Physiology and Seed Science, University of Agriculture, Podłużna 3, 30-239 Kraków, PolandDepartment of Plant Breeding, Physiology and Seed Science, University of Agriculture, Podłużna 3, 30-239 Kraków, PolandDepartment of Plant Breeding, Physiology and Seed Science, University of Agriculture, Podłużna 3, 30-239 Kraków, PolandCommon buckwheat (<i>Fagopyrum esculentum</i> Moench), a pseudocereal crop, produces a large number of flowers, but this does not guarantee high seed yields. This species demonstrates strong abortion of flowers and embryos. High temperatures during the generative growth phase result in an increase in the degeneration of embryo sacs. The aim of this study was to investigate proteomic changes in flowers and leaves of two common buckwheat accessions with different degrees of heat tolerance, Panda and PA15. Two-dimensional gel electrophoresis and mass spectrometry techniques were used to analyze the proteome profiles. Analyses were conducted for flower buds, open flowers capable of fertilization, and wilted flowers, as well as donor leaves, i.e., those growing closest to the inflorescences. High temperature up-regulated the expression of 182 proteins. The proteomic response to heat stress differed between the accessions and among their organs. In the Panda accession, we observed a change in abundance of 17, 13, 28, and 11 proteins, in buds, open and wilted flowers, and leaves, respectively. However, in the PA15 accession there were 34, 21, 63, and 21 such proteins, respectively. Fifteen heat-affected proteins were common to both accessions. The indole-3-glycerol phosphate synthase chloroplastic-like isoform X2 accumulated in the open flowers of the heat-sensitive cultivar Panda in response to high temperature, and may be a candidate protein as a marker of heat sensitivity in buckwheat plants.https://www.mdpi.com/1422-0067/22/5/2678common buckwheathigh temperatureproteomicsheat-affected proteins |
| spellingShingle | Przemysław Kopeć Marta Hornyák Jakub Pastuszak Anna Szczerba Marcin Rapacz Jacek Waga Agnieszka Płażek Changes in the Flower and Leaf Proteome of Common Buckwheat (<i>Fagopyrum esculentum</i> Moench) under High Temperature International Journal of Molecular Sciences common buckwheat high temperature proteomics heat-affected proteins |
| title | Changes in the Flower and Leaf Proteome of Common Buckwheat (<i>Fagopyrum esculentum</i> Moench) under High Temperature |
| title_full | Changes in the Flower and Leaf Proteome of Common Buckwheat (<i>Fagopyrum esculentum</i> Moench) under High Temperature |
| title_fullStr | Changes in the Flower and Leaf Proteome of Common Buckwheat (<i>Fagopyrum esculentum</i> Moench) under High Temperature |
| title_full_unstemmed | Changes in the Flower and Leaf Proteome of Common Buckwheat (<i>Fagopyrum esculentum</i> Moench) under High Temperature |
| title_short | Changes in the Flower and Leaf Proteome of Common Buckwheat (<i>Fagopyrum esculentum</i> Moench) under High Temperature |
| title_sort | changes in the flower and leaf proteome of common buckwheat i fagopyrum esculentum i moench under high temperature |
| topic | common buckwheat high temperature proteomics heat-affected proteins |
| url | https://www.mdpi.com/1422-0067/22/5/2678 |
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