Isothermal amplification of environmental DNA (eDNA) for direct field-based monitoring and laboratory confirmation of Dreissena sp.

Isothermal amplification of environmental DNA (eDNA) for direct field-based monitoring and laboratory confirmation of Dreissena sp.

Loop-mediated isothermal amplification (LAMP) of aquatic invasive species environmental DNA (AIS eDNA) was used for rapid, sensitive, and specific detection of Dreissena sp. relevant to the Great Lakes (USA) basin. The method was validated for two uses including i) direct amplification of eDNA using...

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Bibliographic Details
Main Authors: Maggie R Williams, Robert D Stedtfeld, Cathrine Engle, Paul Salach, Umama Fakher, Tiffany Stedtfeld, Erin Dreelin, R Jan Stevenson, Jo Latimore, Syed A Hashsham
Format: Article
Language:English
Published: Public Library of Science (PLoS) 2017-01-01
Series:PLoS ONE
Online Access:http://europepmc.org/articles/PMC5643059?pdf=render

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