Protocol for observing microtubules and microtubule ends in both fixed and live primary microglia cells
Summary: Microtubule dynamics and orientation have crucial roles in many vital cellular processes. However, functional live imaging of microtubules and/or microtubule ends in primary microglia can be challenging. Here, we present a protocol for observing microtubules and microtubule ends in both fix...
Main Authors: | Caterina Sanchini, Maria Rosito, Alessandro Comincini, Simone De Panfilis, Francesca Bartolini, Silvia Di Angelantonio |
---|---|
Format: | Article |
Language: | English |
Published: |
Elsevier
2023-09-01
|
Series: | STAR Protocols |
Subjects: | |
Online Access: | http://www.sciencedirect.com/science/article/pii/S2666166723004665 |
Similar Items
-
Microglia reactivity entails microtubule remodeling from acentrosomal to centrosomal arrays
by: Maria Rosito, et al.
Published: (2023-02-01) -
Tissue clearing and three-dimensional imaging of the whole cochlea and vestibular system from multiple large-animal models
by: Adele Moatti, et al.
Published: (2023-06-01) -
Protocol to assess extravasation of fluorescent molecules in mice after ultrasound-mediated blood-brain barrier opening
by: Lea Peko, et al.
Published: (2024-03-01) -
Automatic quantification of myocardial remodeling features in human ventricular tissue from label-free microscopy
by: Laura García-Mendívil, et al.
Published: (2023-12-01) -
Construction of a high-NFOM multiphoton microscope with large-angle resonant raster scanning
by: Bhaskar Jyoti Borah, et al.
Published: (2022-06-01)