MiR-598-5p inhibits breast cancer tumor growth and lung metastasis by targeting PPAPDC1A
This study aimed to explore the effects of PPAPDC1A on the malignant phenotype of breast cancer (BC) in vivo and in vitro. PPAPDC1A expression was examined in BC tissues and cell lines by real-time polymerase chain reaction and Western blot. In this article, cell proliferation was evaluated by Cell...
| Main Authors: | , , , , , |
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| Format: | Article |
| Language: | English |
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Wolters Kluwer Medknow Publications
2023-01-01
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| Series: | Chinese Journal of Physiology |
| Subjects: | |
| Online Access: | http://www.cjphysiology.org/article.asp?issn=0304-4920;year=2023;volume=66;issue=2;spage=103;epage=110;aulast=Guo |
| _version_ | 1797824448567443456 |
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| author | Xinyi Guo Fan Yang Liangfei Yu Ronglan Wen Xin Zhang Hui Lin |
| author_facet | Xinyi Guo Fan Yang Liangfei Yu Ronglan Wen Xin Zhang Hui Lin |
| author_sort | Xinyi Guo |
| collection | DOAJ |
| description | This study aimed to explore the effects of PPAPDC1A on the malignant phenotype of breast cancer (BC) in vivo and in vitro. PPAPDC1A expression was examined in BC tissues and cell lines by real-time polymerase chain reaction and Western blot. In this article, cell proliferation was evaluated by Cell Counting Kit-8 assay and colony formation assay, and cell migration and invasion were evaluated by wound healing assay and transwell assays. Furthermore, in vivo cell growth and pulmonary metastasis experiments were also performed using nude mice. The results showed that compared with normal tissues and cells, the PPAPDC1A expression in BC tissues and cell lines were both significantly increased. The PPAPDC1A targeting sequence significantly inhibited the PPAPDC1A expression and cell proliferation, migration, and invasion. The results of xenograft showed that knockdown of PPAPDC1A inhibited tumor growth and lung metastasis of BC. Then, the Dual-Luciferase Reporter Assay confirmed that miR-598-5p targeted the regulation of PPAPDC1A expression. In addition, the miR-598-5p expression in BC tissues was lower than that in the normal tissues. The rescue experiment showed that PPAPDC1A overexpression reversed the inhibitory effect of miR-598-5p mimic on cell proliferation, migration, and invasion. In conclusion, PPAPDC1A was highly expressed in BC tissues and cell lines, and miR-598-5p inhibited the malignant phenotype of BC by targeting PPAPDC1A. |
| first_indexed | 2024-03-13T10:39:07Z |
| format | Article |
| id | doaj.art-838f8e0b24244dee81e334f802082c5b |
| institution | Directory Open Access Journal |
| issn | 0304-4920 2666-0059 |
| language | English |
| last_indexed | 2024-03-13T10:39:07Z |
| publishDate | 2023-01-01 |
| publisher | Wolters Kluwer Medknow Publications |
| record_format | Article |
| series | Chinese Journal of Physiology |
| spelling | doaj.art-838f8e0b24244dee81e334f802082c5b2023-05-18T05:04:32ZengWolters Kluwer Medknow PublicationsChinese Journal of Physiology0304-49202666-00592023-01-0166210311010.4103/cjop.CJOP-D-22-00089MiR-598-5p inhibits breast cancer tumor growth and lung metastasis by targeting PPAPDC1AXinyi GuoFan YangLiangfei YuRonglan WenXin ZhangHui LinThis study aimed to explore the effects of PPAPDC1A on the malignant phenotype of breast cancer (BC) in vivo and in vitro. PPAPDC1A expression was examined in BC tissues and cell lines by real-time polymerase chain reaction and Western blot. In this article, cell proliferation was evaluated by Cell Counting Kit-8 assay and colony formation assay, and cell migration and invasion were evaluated by wound healing assay and transwell assays. Furthermore, in vivo cell growth and pulmonary metastasis experiments were also performed using nude mice. The results showed that compared with normal tissues and cells, the PPAPDC1A expression in BC tissues and cell lines were both significantly increased. The PPAPDC1A targeting sequence significantly inhibited the PPAPDC1A expression and cell proliferation, migration, and invasion. The results of xenograft showed that knockdown of PPAPDC1A inhibited tumor growth and lung metastasis of BC. Then, the Dual-Luciferase Reporter Assay confirmed that miR-598-5p targeted the regulation of PPAPDC1A expression. In addition, the miR-598-5p expression in BC tissues was lower than that in the normal tissues. The rescue experiment showed that PPAPDC1A overexpression reversed the inhibitory effect of miR-598-5p mimic on cell proliferation, migration, and invasion. In conclusion, PPAPDC1A was highly expressed in BC tissues and cell lines, and miR-598-5p inhibited the malignant phenotype of BC by targeting PPAPDC1A.http://www.cjphysiology.org/article.asp?issn=0304-4920;year=2023;volume=66;issue=2;spage=103;epage=110;aulast=Guobreast cancerlung metastasismir-598-5pppapdc1a |
| spellingShingle | Xinyi Guo Fan Yang Liangfei Yu Ronglan Wen Xin Zhang Hui Lin MiR-598-5p inhibits breast cancer tumor growth and lung metastasis by targeting PPAPDC1A Chinese Journal of Physiology breast cancer lung metastasis mir-598-5p ppapdc1a |
| title | MiR-598-5p inhibits breast cancer tumor growth and lung metastasis by targeting PPAPDC1A |
| title_full | MiR-598-5p inhibits breast cancer tumor growth and lung metastasis by targeting PPAPDC1A |
| title_fullStr | MiR-598-5p inhibits breast cancer tumor growth and lung metastasis by targeting PPAPDC1A |
| title_full_unstemmed | MiR-598-5p inhibits breast cancer tumor growth and lung metastasis by targeting PPAPDC1A |
| title_short | MiR-598-5p inhibits breast cancer tumor growth and lung metastasis by targeting PPAPDC1A |
| title_sort | mir 598 5p inhibits breast cancer tumor growth and lung metastasis by targeting ppapdc1a |
| topic | breast cancer lung metastasis mir-598-5p ppapdc1a |
| url | http://www.cjphysiology.org/article.asp?issn=0304-4920;year=2023;volume=66;issue=2;spage=103;epage=110;aulast=Guo |
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