The Impact of Human Lipoaspirate and Adipose Tissue-Derived Stem Cells Contact Culture on Breast Cancer Cells: Implications in Breast Reconstruction
Background: Autologous fat transfer in the form of lipoaspirates for the reconstruction of the breast after breast cancer surgery is a commonly used procedure in plastic surgery. However, concerns regarding the oncologic risk of nutrient-rich fat tissue are widely debated. Previous studies have prim...
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| Format: | Article |
| Language: | English |
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MDPI AG
2020-12-01
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| Series: | International Journal of Molecular Sciences |
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| Online Access: | https://www.mdpi.com/1422-0067/21/23/9171 |
| _version_ | 1797546036160364544 |
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| author | Asim Ejaz Katherine S. Yang Kaushik P. Venkatesh Somaiah Chinnapaka Lauren E. Kokai J. Peter Rubin |
| author_facet | Asim Ejaz Katherine S. Yang Kaushik P. Venkatesh Somaiah Chinnapaka Lauren E. Kokai J. Peter Rubin |
| author_sort | Asim Ejaz |
| collection | DOAJ |
| description | Background: Autologous fat transfer in the form of lipoaspirates for the reconstruction of the breast after breast cancer surgery is a commonly used procedure in plastic surgery. However, concerns regarding the oncologic risk of nutrient-rich fat tissue are widely debated. Previous studies have primarily focused on studying the interaction between adipose-derived stem cells (ASCs) and breast cancer cells. Methods: In this study, we performed a comprehensive analysis of the paracrine- and contact-based interactions between lipoaspirates, ASCs and breast cancer cell lines. An inverted flask culture method was used to study the contact-based interaction between lipoaspirates and breast cancer cells, while GFP-expressing breast cancer cell lines were generated to study the cell–cell contact interaction with ASCs. Three different human breast cancer cell lines, MCF-7, MDA-MB-231 and BT-474, were studied. We analyzed the impact of these interactions on the proliferation, cell cycle and epithelial-to-mesenchymal (EMT) transition of the breast cancer cells. Results: Our results revealed that both lipoaspirates and ASCs do not increase the proliferation rate of the breast cancer cells either through paracrine- or contact-dependent interactions. We observed that lipoaspirates selectively inhibit the proliferation of MCF-7 cells in contact co-culture, driven by the retinoblastoma (Rb) protein activity mediating cell cycle arrest. Additionally, ASCs inhibited MDA-MB-231 breast cancer cell proliferation in cell–cell contact-dependent interactions. Quantitative real-time PCR revealed no significant increase in the EMT-related genes in breast cancer cells upon co-culture with ASCs. Conclusion: In conclusion, this study provides evidence of the non-oncogenic character of lipoaspirates and supports the safety of clinical fat grafting in breast reconstruction after oncological surgical procedures. In vivo studies in appropriate animal models and long-term post-operative clinical data from patients are essential to reach the final safety recommendations. |
| first_indexed | 2024-03-10T14:24:23Z |
| format | Article |
| id | doaj.art-8392b80e0da1428b88f80db186b30833 |
| institution | Directory Open Access Journal |
| issn | 1661-6596 1422-0067 |
| language | English |
| last_indexed | 2024-03-10T14:24:23Z |
| publishDate | 2020-12-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | International Journal of Molecular Sciences |
| spelling | doaj.art-8392b80e0da1428b88f80db186b308332023-11-20T23:08:59ZengMDPI AGInternational Journal of Molecular Sciences1661-65961422-00672020-12-012123917110.3390/ijms21239171The Impact of Human Lipoaspirate and Adipose Tissue-Derived Stem Cells Contact Culture on Breast Cancer Cells: Implications in Breast ReconstructionAsim Ejaz0Katherine S. Yang1Kaushik P. Venkatesh2Somaiah Chinnapaka3Lauren E. Kokai4J. Peter Rubin5Department of Plastic Surgery, University of Pittsburgh, Pittsburgh, PA 15261, USADepartment of Plastic Surgery, University of Pittsburgh, Pittsburgh, PA 15261, USADepartment of Plastic Surgery, University of Pittsburgh, Pittsburgh, PA 15261, USADepartment of Plastic Surgery, University of Pittsburgh, Pittsburgh, PA 15261, USADepartment of Plastic Surgery, University of Pittsburgh, Pittsburgh, PA 15261, USADepartment of Plastic Surgery, University of Pittsburgh, Pittsburgh, PA 15261, USABackground: Autologous fat transfer in the form of lipoaspirates for the reconstruction of the breast after breast cancer surgery is a commonly used procedure in plastic surgery. However, concerns regarding the oncologic risk of nutrient-rich fat tissue are widely debated. Previous studies have primarily focused on studying the interaction between adipose-derived stem cells (ASCs) and breast cancer cells. Methods: In this study, we performed a comprehensive analysis of the paracrine- and contact-based interactions between lipoaspirates, ASCs and breast cancer cell lines. An inverted flask culture method was used to study the contact-based interaction between lipoaspirates and breast cancer cells, while GFP-expressing breast cancer cell lines were generated to study the cell–cell contact interaction with ASCs. Three different human breast cancer cell lines, MCF-7, MDA-MB-231 and BT-474, were studied. We analyzed the impact of these interactions on the proliferation, cell cycle and epithelial-to-mesenchymal (EMT) transition of the breast cancer cells. Results: Our results revealed that both lipoaspirates and ASCs do not increase the proliferation rate of the breast cancer cells either through paracrine- or contact-dependent interactions. We observed that lipoaspirates selectively inhibit the proliferation of MCF-7 cells in contact co-culture, driven by the retinoblastoma (Rb) protein activity mediating cell cycle arrest. Additionally, ASCs inhibited MDA-MB-231 breast cancer cell proliferation in cell–cell contact-dependent interactions. Quantitative real-time PCR revealed no significant increase in the EMT-related genes in breast cancer cells upon co-culture with ASCs. Conclusion: In conclusion, this study provides evidence of the non-oncogenic character of lipoaspirates and supports the safety of clinical fat grafting in breast reconstruction after oncological surgical procedures. In vivo studies in appropriate animal models and long-term post-operative clinical data from patients are essential to reach the final safety recommendations.https://www.mdpi.com/1422-0067/21/23/9171adipose-derived stem cellslipoaspiratesbreast cancer cellscell cycle and proliferation |
| spellingShingle | Asim Ejaz Katherine S. Yang Kaushik P. Venkatesh Somaiah Chinnapaka Lauren E. Kokai J. Peter Rubin The Impact of Human Lipoaspirate and Adipose Tissue-Derived Stem Cells Contact Culture on Breast Cancer Cells: Implications in Breast Reconstruction International Journal of Molecular Sciences adipose-derived stem cells lipoaspirates breast cancer cells cell cycle and proliferation |
| title | The Impact of Human Lipoaspirate and Adipose Tissue-Derived Stem Cells Contact Culture on Breast Cancer Cells: Implications in Breast Reconstruction |
| title_full | The Impact of Human Lipoaspirate and Adipose Tissue-Derived Stem Cells Contact Culture on Breast Cancer Cells: Implications in Breast Reconstruction |
| title_fullStr | The Impact of Human Lipoaspirate and Adipose Tissue-Derived Stem Cells Contact Culture on Breast Cancer Cells: Implications in Breast Reconstruction |
| title_full_unstemmed | The Impact of Human Lipoaspirate and Adipose Tissue-Derived Stem Cells Contact Culture on Breast Cancer Cells: Implications in Breast Reconstruction |
| title_short | The Impact of Human Lipoaspirate and Adipose Tissue-Derived Stem Cells Contact Culture on Breast Cancer Cells: Implications in Breast Reconstruction |
| title_sort | impact of human lipoaspirate and adipose tissue derived stem cells contact culture on breast cancer cells implications in breast reconstruction |
| topic | adipose-derived stem cells lipoaspirates breast cancer cells cell cycle and proliferation |
| url | https://www.mdpi.com/1422-0067/21/23/9171 |
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