Transcriptome Profiling Reveals Differential Gene Expression of Laccase Genes in <i>Aspergillus terreus</i> KC462061 during Biodegradation of Crude Oil
Fungal laccases have high catalytic efficiency and are utilized for the removal of crude oil because they oxidize various aliphatic and aromatic hydrocarbons and convert them into harmless compounds or less toxic compounds, thus accelerating the biodegradation potential of crude oil. Laccases are im...
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MDPI AG
2022-04-01
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| author | Nada K. Alharbi Mayasar I. Alzaban Fawziah M. Albarakaty Abeer R. M. Abd El-Aziz Ahlam H. AlRokban Mohamed A. Mahmoud |
| author_facet | Nada K. Alharbi Mayasar I. Alzaban Fawziah M. Albarakaty Abeer R. M. Abd El-Aziz Ahlam H. AlRokban Mohamed A. Mahmoud |
| author_sort | Nada K. Alharbi |
| collection | DOAJ |
| description | Fungal laccases have high catalytic efficiency and are utilized for the removal of crude oil because they oxidize various aliphatic and aromatic hydrocarbons and convert them into harmless compounds or less toxic compounds, thus accelerating the biodegradation potential of crude oil. Laccases are important gene families and the function of laccases genes varied widely based on transcription and function. Biodegradation of crude oil using <i>Aspergillus terreus</i> KC462061 was studied in the current study beside the transcription level of eight laccase (<i>Lcc</i>) genes have participated in biodegradation in the presence of aromatic compounds, and metal ions. Time-course profiles of laccase activity in the presence of crude oil indicated that the five inducers individual or combined have a very positive on laccase activity. In the status of the existence of crude oil, the synergistic effect of Cu-ABTS compound caused an increase in laccase yields up to 22-fold after 10 days than control. The biodegradation efficiencies of <i>A. terreus</i> KC462061 for aliphatic and aromatic hydrocarbons of crude oil were 82.1 ± 0.2% and 77.4 ± 0.6%, respectively. The crude oil biodegradation efficiency was improved by the supplemented Cu-ABTS compound in <i>A. terreus</i> KC462061. Gas chromatography–mass spectrometry was a very accurate tool to demonstrate the biodegradation efficiencies of <i>A. terreus</i> KC462061 for crude oil. Significant differences were observed in the SDS-PAGE of <i>A. terreus</i> KC462061 band intensities of laccase proteins after the addition of five inducers, but the Cu-ABTS compound highly affects very particular laccase electrophoresis. Quantitative real-time polymerase chain reaction (qPCR) was used for the analysis of transcription profile of eight laccase genes in <i>A. terreus</i> KC462061 with a verified reference gene. Cu<sup>2+</sup> ions and Cu-ABTS were highly effective for efficient laccase expression profiling, mainly via <i>Lcc11</i> and <i>12</i> transcription induction. The current study will explain the theoretical foundation for laccase transcription in <i>A. terreus</i> KC462061, paving the road for commercialization and usage. |
| first_indexed | 2024-03-09T11:08:14Z |
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| last_indexed | 2024-03-09T11:08:14Z |
| publishDate | 2022-04-01 |
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| spelling | doaj.art-839ef406f8d448be914828faa8b1876f2023-12-01T00:51:36ZengMDPI AGBiology2079-77372022-04-0111456410.3390/biology11040564Transcriptome Profiling Reveals Differential Gene Expression of Laccase Genes in <i>Aspergillus terreus</i> KC462061 during Biodegradation of Crude OilNada K. Alharbi0Mayasar I. Alzaban1Fawziah M. Albarakaty2Abeer R. M. Abd El-Aziz3Ahlam H. AlRokban4Mohamed A. Mahmoud5Department of Biology, College of Science, Princess Nourah bint Abdulrahman University, P.O. Box 84428, Riyadh 11671, Saudi ArabiaDepartment of Biology, College of Science, Princess Nourah bint Abdulrahman University, P.O. Box 84428, Riyadh 11671, Saudi ArabiaDepartment of Biology, Faculty of Applied Science, Umm Al-Qura University, P.O. Box 715, Makkah Al Mukarramah 21955, Saudi ArabiaBotany and Microbiology Department, College of Science, King Saud University, Riyadh 11451, Saudi ArabiaDepartment of Biology, College of Science, Princess Nourah bint Abdulrahman University, P.O. Box 84428, Riyadh 11671, Saudi ArabiaMolecular Markers Laboratory, Plant Pathology Research Institute, Agricultural Research Center, Giza 12619, EgyptFungal laccases have high catalytic efficiency and are utilized for the removal of crude oil because they oxidize various aliphatic and aromatic hydrocarbons and convert them into harmless compounds or less toxic compounds, thus accelerating the biodegradation potential of crude oil. Laccases are important gene families and the function of laccases genes varied widely based on transcription and function. Biodegradation of crude oil using <i>Aspergillus terreus</i> KC462061 was studied in the current study beside the transcription level of eight laccase (<i>Lcc</i>) genes have participated in biodegradation in the presence of aromatic compounds, and metal ions. Time-course profiles of laccase activity in the presence of crude oil indicated that the five inducers individual or combined have a very positive on laccase activity. In the status of the existence of crude oil, the synergistic effect of Cu-ABTS compound caused an increase in laccase yields up to 22-fold after 10 days than control. The biodegradation efficiencies of <i>A. terreus</i> KC462061 for aliphatic and aromatic hydrocarbons of crude oil were 82.1 ± 0.2% and 77.4 ± 0.6%, respectively. The crude oil biodegradation efficiency was improved by the supplemented Cu-ABTS compound in <i>A. terreus</i> KC462061. Gas chromatography–mass spectrometry was a very accurate tool to demonstrate the biodegradation efficiencies of <i>A. terreus</i> KC462061 for crude oil. Significant differences were observed in the SDS-PAGE of <i>A. terreus</i> KC462061 band intensities of laccase proteins after the addition of five inducers, but the Cu-ABTS compound highly affects very particular laccase electrophoresis. Quantitative real-time polymerase chain reaction (qPCR) was used for the analysis of transcription profile of eight laccase genes in <i>A. terreus</i> KC462061 with a verified reference gene. Cu<sup>2+</sup> ions and Cu-ABTS were highly effective for efficient laccase expression profiling, mainly via <i>Lcc11</i> and <i>12</i> transcription induction. The current study will explain the theoretical foundation for laccase transcription in <i>A. terreus</i> KC462061, paving the road for commercialization and usage.https://www.mdpi.com/2079-7737/11/4/564<i>Aspergillus terreus</i> KC462061biodegradationqPCRlaccasedifferential regulationpromoter |
| spellingShingle | Nada K. Alharbi Mayasar I. Alzaban Fawziah M. Albarakaty Abeer R. M. Abd El-Aziz Ahlam H. AlRokban Mohamed A. Mahmoud Transcriptome Profiling Reveals Differential Gene Expression of Laccase Genes in <i>Aspergillus terreus</i> KC462061 during Biodegradation of Crude Oil Biology <i>Aspergillus terreus</i> KC462061 biodegradation qPCR laccase differential regulation promoter |
| title | Transcriptome Profiling Reveals Differential Gene Expression of Laccase Genes in <i>Aspergillus terreus</i> KC462061 during Biodegradation of Crude Oil |
| title_full | Transcriptome Profiling Reveals Differential Gene Expression of Laccase Genes in <i>Aspergillus terreus</i> KC462061 during Biodegradation of Crude Oil |
| title_fullStr | Transcriptome Profiling Reveals Differential Gene Expression of Laccase Genes in <i>Aspergillus terreus</i> KC462061 during Biodegradation of Crude Oil |
| title_full_unstemmed | Transcriptome Profiling Reveals Differential Gene Expression of Laccase Genes in <i>Aspergillus terreus</i> KC462061 during Biodegradation of Crude Oil |
| title_short | Transcriptome Profiling Reveals Differential Gene Expression of Laccase Genes in <i>Aspergillus terreus</i> KC462061 during Biodegradation of Crude Oil |
| title_sort | transcriptome profiling reveals differential gene expression of laccase genes in i aspergillus terreus i kc462061 during biodegradation of crude oil |
| topic | <i>Aspergillus terreus</i> KC462061 biodegradation qPCR laccase differential regulation promoter |
| url | https://www.mdpi.com/2079-7737/11/4/564 |
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