Application of Hyperspectral Imaging to Detect Sclerotinia sclerotiorum on Oilseed Rape Stems

Hyperspectral imaging covering the spectral range of 384–1034 nm combined with chemometric methods was used to detect Sclerotinia sclerotiorum (SS) on oilseed rape stems by two sample sets (60 healthy and 60 infected stems for each set). Second derivative spectra and PCA loadings were used to select...

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Main Authors: Wenwen Kong, Chu Zhang, Weihao Huang, Fei Liu, Yong He
Format: Article
Language:English
Published: MDPI AG 2018-01-01
Series:Sensors
Subjects:
Online Access:http://www.mdpi.com/1424-8220/18/1/123
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author Wenwen Kong
Chu Zhang
Weihao Huang
Fei Liu
Yong He
author_facet Wenwen Kong
Chu Zhang
Weihao Huang
Fei Liu
Yong He
author_sort Wenwen Kong
collection DOAJ
description Hyperspectral imaging covering the spectral range of 384–1034 nm combined with chemometric methods was used to detect Sclerotinia sclerotiorum (SS) on oilseed rape stems by two sample sets (60 healthy and 60 infected stems for each set). Second derivative spectra and PCA loadings were used to select the optimal wavelengths. Discriminant models were built and compared to detect SS on oilseed rape stems, including partial least squares-discriminant analysis, radial basis function neural network, support vector machine and extreme learning machine. The discriminant models using full spectra and optimal wavelengths showed good performance with classification accuracies of over 80% for the calibration and prediction set. Comparing all developed models, the optimal classification accuracies of the calibration and prediction set were over 90%. The similarity of selected optimal wavelengths also indicated the feasibility of using hyperspectral imaging to detect SS on oilseed rape stems. The results indicated that hyperspectral imaging could be used as a fast, non-destructive and reliable technique to detect plant diseases on stems.
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spelling doaj.art-83a023880aae472590550584287418cc2022-12-22T02:19:22ZengMDPI AGSensors1424-82202018-01-0118112310.3390/s18010123s18010123Application of Hyperspectral Imaging to Detect Sclerotinia sclerotiorum on Oilseed Rape StemsWenwen Kong0Chu Zhang1Weihao Huang2Fei Liu3Yong He4School of Information Engineering, Zhejiang A & F University, 666 Wusu Street, Hangzhou 311300, ChinaCollege of Biosystems Engineering and Food Science, Zhejiang University, Hangzhou 310058, ChinaCollege of Biosystems Engineering and Food Science, Zhejiang University, Hangzhou 310058, ChinaCollege of Biosystems Engineering and Food Science, Zhejiang University, Hangzhou 310058, ChinaCollege of Biosystems Engineering and Food Science, Zhejiang University, Hangzhou 310058, ChinaHyperspectral imaging covering the spectral range of 384–1034 nm combined with chemometric methods was used to detect Sclerotinia sclerotiorum (SS) on oilseed rape stems by two sample sets (60 healthy and 60 infected stems for each set). Second derivative spectra and PCA loadings were used to select the optimal wavelengths. Discriminant models were built and compared to detect SS on oilseed rape stems, including partial least squares-discriminant analysis, radial basis function neural network, support vector machine and extreme learning machine. The discriminant models using full spectra and optimal wavelengths showed good performance with classification accuracies of over 80% for the calibration and prediction set. Comparing all developed models, the optimal classification accuracies of the calibration and prediction set were over 90%. The similarity of selected optimal wavelengths also indicated the feasibility of using hyperspectral imaging to detect SS on oilseed rape stems. The results indicated that hyperspectral imaging could be used as a fast, non-destructive and reliable technique to detect plant diseases on stems.http://www.mdpi.com/1424-8220/18/1/123oilseed rape stemSclerotinia sclerotiorumsecond derivative spectradiscriminant models
spellingShingle Wenwen Kong
Chu Zhang
Weihao Huang
Fei Liu
Yong He
Application of Hyperspectral Imaging to Detect Sclerotinia sclerotiorum on Oilseed Rape Stems
Sensors
oilseed rape stem
Sclerotinia sclerotiorum
second derivative spectra
discriminant models
title Application of Hyperspectral Imaging to Detect Sclerotinia sclerotiorum on Oilseed Rape Stems
title_full Application of Hyperspectral Imaging to Detect Sclerotinia sclerotiorum on Oilseed Rape Stems
title_fullStr Application of Hyperspectral Imaging to Detect Sclerotinia sclerotiorum on Oilseed Rape Stems
title_full_unstemmed Application of Hyperspectral Imaging to Detect Sclerotinia sclerotiorum on Oilseed Rape Stems
title_short Application of Hyperspectral Imaging to Detect Sclerotinia sclerotiorum on Oilseed Rape Stems
title_sort application of hyperspectral imaging to detect sclerotinia sclerotiorum on oilseed rape stems
topic oilseed rape stem
Sclerotinia sclerotiorum
second derivative spectra
discriminant models
url http://www.mdpi.com/1424-8220/18/1/123
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