Prolonged post-thaw culture of embryos does not improve outcomes of frozen human embryo transfer cycles: A prospective randomized study
Objective: To evaluate the impact of prolonged post-thaw embryos culture on pregnancy outcome during frozen embryo transfer cycles. Methods: This prospective cohort study evaluated 324 thaw transfer cycles with 819 embryos from 269 patients at the Center for Reproductive Endocrinology and Infertilit...
| Main Authors: | , , , , , , , , , |
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| Format: | Article |
| Language: | English |
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Wolters Kluwer Medknow Publications
2019-01-01
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| Series: | Asian Pacific Journal of Reproduction |
| Subjects: | |
| Online Access: | http://www.apjr.net/article.asp?issn=2305-0500;year=2019;volume=8;issue=6;spage=260;epage=266;aulast=Le |
| _version_ | 1811234579865927680 |
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| author | Minh Tam Le Van Trung Nguyen Thanh Tung Nguyen M Blake Evans Thai Thanh Thi Nguyen Tam An Thi Nguyen Dinh Duong Le Vu Quoc Huy Nguyen Ngoc Thanh Cao Micah J Hill |
| author_facet | Minh Tam Le Van Trung Nguyen Thanh Tung Nguyen M Blake Evans Thai Thanh Thi Nguyen Tam An Thi Nguyen Dinh Duong Le Vu Quoc Huy Nguyen Ngoc Thanh Cao Micah J Hill |
| author_sort | Minh Tam Le |
| collection | DOAJ |
| description | Objective: To evaluate the impact of prolonged post-thaw embryos culture on pregnancy outcome during frozen embryo transfer cycles.
Methods: This prospective cohort study evaluated 324 thaw transfer cycles with 819 embryos from 269 patients at the Center for Reproductive Endocrinology and Infertility of Hue University Hospital in Vietnam. These frozen embryo transfer cycles were divided into two groups at the time of thawing: the short culture group (2-hour post-thaw culture) and the overnight culture group (overnight culture for 18 h) before the embryo was transferred into the uterus. The rates of embryo intact, grade A embryo at frozen and transfer time and continuing cleavage were recorded. The clinical outcomes including serum beta-human chorionic gonadotropin, clinical pregnancy and implantation rate were evaluated after 14 days, 4 weeks, 6 weeks, respectively, after embryo transfer.
Results: Human chorionic gonadotropin positive occurred in 39.5% of patients in the short culture group compared to 25.9% in the overnight culture group with risk difference (RD)=13.6%, relative risk (RR)=1.343, 95% confidence interval (CI) 1.085-1.663, P<0.01. Clinical pregnancy rate of the short culture group and overnight culture group was 33.3% and 24.1%, respectively (RD=9.2%, RR=1.242, 95% CI 0.996-1.549, P =0.06) and the implantation rate in the short culture group and overnight culture group was 16.5% and 11.0%, respectively (RD=5.5%, RR=1.244, 95% CI 1.046-1.479, P =0.01). In women of advanced age (>35 years) and women who received 3 embryos, pregnancy outcomes were found to be significantly (P <0.05) higher in the short culture than in the overnight culture group.
Conclusions: The prolonged post-thaw culture period does not increase pregnancy outcome in comparison with the short culture. |
| first_indexed | 2024-04-12T11:38:43Z |
| format | Article |
| id | doaj.art-83a8921f5de34658b93870726f448b9f |
| institution | Directory Open Access Journal |
| issn | 2305-0500 2305-0519 |
| language | English |
| last_indexed | 2024-04-12T11:38:43Z |
| publishDate | 2019-01-01 |
| publisher | Wolters Kluwer Medknow Publications |
| record_format | Article |
| series | Asian Pacific Journal of Reproduction |
| spelling | doaj.art-83a8921f5de34658b93870726f448b9f2022-12-22T03:34:44ZengWolters Kluwer Medknow PublicationsAsian Pacific Journal of Reproduction2305-05002305-05192019-01-018626026610.4103/2305-0500.270103Prolonged post-thaw culture of embryos does not improve outcomes of frozen human embryo transfer cycles: A prospective randomized studyMinh Tam LeVan Trung NguyenThanh Tung NguyenM Blake EvansThai Thanh Thi NguyenTam An Thi NguyenDinh Duong LeVu Quoc Huy NguyenNgoc Thanh CaoMicah J HillObjective: To evaluate the impact of prolonged post-thaw embryos culture on pregnancy outcome during frozen embryo transfer cycles. Methods: This prospective cohort study evaluated 324 thaw transfer cycles with 819 embryos from 269 patients at the Center for Reproductive Endocrinology and Infertility of Hue University Hospital in Vietnam. These frozen embryo transfer cycles were divided into two groups at the time of thawing: the short culture group (2-hour post-thaw culture) and the overnight culture group (overnight culture for 18 h) before the embryo was transferred into the uterus. The rates of embryo intact, grade A embryo at frozen and transfer time and continuing cleavage were recorded. The clinical outcomes including serum beta-human chorionic gonadotropin, clinical pregnancy and implantation rate were evaluated after 14 days, 4 weeks, 6 weeks, respectively, after embryo transfer. Results: Human chorionic gonadotropin positive occurred in 39.5% of patients in the short culture group compared to 25.9% in the overnight culture group with risk difference (RD)=13.6%, relative risk (RR)=1.343, 95% confidence interval (CI) 1.085-1.663, P<0.01. Clinical pregnancy rate of the short culture group and overnight culture group was 33.3% and 24.1%, respectively (RD=9.2%, RR=1.242, 95% CI 0.996-1.549, P =0.06) and the implantation rate in the short culture group and overnight culture group was 16.5% and 11.0%, respectively (RD=5.5%, RR=1.244, 95% CI 1.046-1.479, P =0.01). In women of advanced age (>35 years) and women who received 3 embryos, pregnancy outcomes were found to be significantly (P <0.05) higher in the short culture than in the overnight culture group. Conclusions: The prolonged post-thaw culture period does not increase pregnancy outcome in comparison with the short culture.http://www.apjr.net/article.asp?issn=2305-0500;year=2019;volume=8;issue=6;spage=260;epage=266;aulast=Leembryo vitrificationpost-thaw culturefrozen embryo transferbiochemical pregnancy rateclinical pregnancy rateimplantation rate |
| spellingShingle | Minh Tam Le Van Trung Nguyen Thanh Tung Nguyen M Blake Evans Thai Thanh Thi Nguyen Tam An Thi Nguyen Dinh Duong Le Vu Quoc Huy Nguyen Ngoc Thanh Cao Micah J Hill Prolonged post-thaw culture of embryos does not improve outcomes of frozen human embryo transfer cycles: A prospective randomized study Asian Pacific Journal of Reproduction embryo vitrification post-thaw culture frozen embryo transfer biochemical pregnancy rate clinical pregnancy rate implantation rate |
| title | Prolonged post-thaw culture of embryos does not improve outcomes of frozen human embryo transfer cycles: A prospective randomized study |
| title_full | Prolonged post-thaw culture of embryos does not improve outcomes of frozen human embryo transfer cycles: A prospective randomized study |
| title_fullStr | Prolonged post-thaw culture of embryos does not improve outcomes of frozen human embryo transfer cycles: A prospective randomized study |
| title_full_unstemmed | Prolonged post-thaw culture of embryos does not improve outcomes of frozen human embryo transfer cycles: A prospective randomized study |
| title_short | Prolonged post-thaw culture of embryos does not improve outcomes of frozen human embryo transfer cycles: A prospective randomized study |
| title_sort | prolonged post thaw culture of embryos does not improve outcomes of frozen human embryo transfer cycles a prospective randomized study |
| topic | embryo vitrification post-thaw culture frozen embryo transfer biochemical pregnancy rate clinical pregnancy rate implantation rate |
| url | http://www.apjr.net/article.asp?issn=2305-0500;year=2019;volume=8;issue=6;spage=260;epage=266;aulast=Le |
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