Prospects of Using Protein Engineering for Selective Drug Delivery into a Specific Compartment of Target Cells
A large number of proteins are successfully used to treat various diseases. These include natural polypeptide hormones, their synthetic analogues, antibodies, antibody mimetics, enzymes, and other drugs based on them. Many of them are demanded in clinical settings and commercially successful, mainly...
| Main Authors: | , |
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| Format: | Article |
| Language: | English |
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MDPI AG
2023-03-01
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| Series: | Pharmaceutics |
| Subjects: | |
| Online Access: | https://www.mdpi.com/1999-4923/15/3/987 |
| _version_ | 1797609505848033280 |
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| author | Andrey A. Rosenkranz Tatiana A. Slastnikova |
| author_facet | Andrey A. Rosenkranz Tatiana A. Slastnikova |
| author_sort | Andrey A. Rosenkranz |
| collection | DOAJ |
| description | A large number of proteins are successfully used to treat various diseases. These include natural polypeptide hormones, their synthetic analogues, antibodies, antibody mimetics, enzymes, and other drugs based on them. Many of them are demanded in clinical settings and commercially successful, mainly for cancer treatment. The targets for most of the aforementioned drugs are located at the cell surface. Meanwhile, the vast majority of therapeutic targets, which are usually regulatory macromolecules, are located inside the cell. Traditional low molecular weight drugs freely penetrate all cells, causing side effects in non-target cells. In addition, it is often difficult to elaborate a small molecule that can specifically affect protein interactions. Modern technologies make it possible to obtain proteins capable of interacting with almost any target. However, proteins, like other macromolecules, cannot, as a rule, freely penetrate into the desired cellular compartment. Recent studies allow us to design multifunctional proteins that solve these problems. This review considers the scope of application of such artificial constructs for the targeted delivery of both protein-based and traditional low molecular weight drugs, the obstacles met on the way of their transport to the specified intracellular compartment of the target cells after their systemic bloodstream administration, and the means to overcome those difficulties. |
| first_indexed | 2024-03-11T06:01:20Z |
| format | Article |
| id | doaj.art-83ada8bdc22148c689c92c7382b905be |
| institution | Directory Open Access Journal |
| issn | 1999-4923 |
| language | English |
| last_indexed | 2024-03-11T06:01:20Z |
| publishDate | 2023-03-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Pharmaceutics |
| spelling | doaj.art-83ada8bdc22148c689c92c7382b905be2023-11-17T13:17:12ZengMDPI AGPharmaceutics1999-49232023-03-0115398710.3390/pharmaceutics15030987Prospects of Using Protein Engineering for Selective Drug Delivery into a Specific Compartment of Target CellsAndrey A. Rosenkranz0Tatiana A. Slastnikova1Laboratory of Molecular Genetics of Intracellular Transport, Institute of Gene Biology of Russian Academy of Sciences, 34/5 Vavilov St., 119334 Moscow, RussiaLaboratory of Molecular Genetics of Intracellular Transport, Institute of Gene Biology of Russian Academy of Sciences, 34/5 Vavilov St., 119334 Moscow, RussiaA large number of proteins are successfully used to treat various diseases. These include natural polypeptide hormones, their synthetic analogues, antibodies, antibody mimetics, enzymes, and other drugs based on them. Many of them are demanded in clinical settings and commercially successful, mainly for cancer treatment. The targets for most of the aforementioned drugs are located at the cell surface. Meanwhile, the vast majority of therapeutic targets, which are usually regulatory macromolecules, are located inside the cell. Traditional low molecular weight drugs freely penetrate all cells, causing side effects in non-target cells. In addition, it is often difficult to elaborate a small molecule that can specifically affect protein interactions. Modern technologies make it possible to obtain proteins capable of interacting with almost any target. However, proteins, like other macromolecules, cannot, as a rule, freely penetrate into the desired cellular compartment. Recent studies allow us to design multifunctional proteins that solve these problems. This review considers the scope of application of such artificial constructs for the targeted delivery of both protein-based and traditional low molecular weight drugs, the obstacles met on the way of their transport to the specified intracellular compartment of the target cells after their systemic bloodstream administration, and the means to overcome those difficulties.https://www.mdpi.com/1999-4923/15/3/987targeted drug deliveryproteinssubcellular transportcancerprotein engineeringmultifunctional proteins |
| spellingShingle | Andrey A. Rosenkranz Tatiana A. Slastnikova Prospects of Using Protein Engineering for Selective Drug Delivery into a Specific Compartment of Target Cells Pharmaceutics targeted drug delivery proteins subcellular transport cancer protein engineering multifunctional proteins |
| title | Prospects of Using Protein Engineering for Selective Drug Delivery into a Specific Compartment of Target Cells |
| title_full | Prospects of Using Protein Engineering for Selective Drug Delivery into a Specific Compartment of Target Cells |
| title_fullStr | Prospects of Using Protein Engineering for Selective Drug Delivery into a Specific Compartment of Target Cells |
| title_full_unstemmed | Prospects of Using Protein Engineering for Selective Drug Delivery into a Specific Compartment of Target Cells |
| title_short | Prospects of Using Protein Engineering for Selective Drug Delivery into a Specific Compartment of Target Cells |
| title_sort | prospects of using protein engineering for selective drug delivery into a specific compartment of target cells |
| topic | targeted drug delivery proteins subcellular transport cancer protein engineering multifunctional proteins |
| url | https://www.mdpi.com/1999-4923/15/3/987 |
| work_keys_str_mv | AT andreyarosenkranz prospectsofusingproteinengineeringforselectivedrugdeliveryintoaspecificcompartmentoftargetcells AT tatianaaslastnikova prospectsofusingproteinengineeringforselectivedrugdeliveryintoaspecificcompartmentoftargetcells |