Low <i>Aedes aegypti</i> Vector Competence for Zika Virus from Viremic Rhesus Macaques
Despite worldwide efforts to understand the transmission dynamics of Zika virus (ZIKV), scanty evaluation has been made on the vector competence of <i>Aedes aegypti</i> fed directly on viremic human and non-human primates (NHPs). We blood-fed <i>Ae. aegypti</i> from two distr...
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| Format: | Article |
| Language: | English |
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MDPI AG
2020-11-01
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| Series: | Viruses |
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| Online Access: | https://www.mdpi.com/1999-4915/12/12/1345 |
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| author | Rosilainy Surubi Fernandes Mariana Rocha David Filipe Vieira Santos De Abreu Anielly Ferreira-de-Brito Noemi R. Gardinali Sheila Maria Barbosa Lima Márcia Cristina Ribeiro Andrade Tatiana Kugelmeier Jaqueline Mendes de Oliveira Marcelo A. Pinto Ricardo Lourenço-de-Oliveira |
| author_facet | Rosilainy Surubi Fernandes Mariana Rocha David Filipe Vieira Santos De Abreu Anielly Ferreira-de-Brito Noemi R. Gardinali Sheila Maria Barbosa Lima Márcia Cristina Ribeiro Andrade Tatiana Kugelmeier Jaqueline Mendes de Oliveira Marcelo A. Pinto Ricardo Lourenço-de-Oliveira |
| author_sort | Rosilainy Surubi Fernandes |
| collection | DOAJ |
| description | Despite worldwide efforts to understand the transmission dynamics of Zika virus (ZIKV), scanty evaluation has been made on the vector competence of <i>Aedes aegypti</i> fed directly on viremic human and non-human primates (NHPs). We blood-fed <i>Ae. aegypti</i> from two districts in Rio de Janeiro on six ZIKV infected pregnant rhesus macaques at several time points, half of which were treated with Sofosbuvir (SOF). Mosquitoes were analyzed for vector competence after 3, 7 and 14 days of incubation. Although viremia extended up to eight days post monkey inoculation, only mosquitoes fed on the day of the peak of viremia, recorded on day two, became infected. The influence of SOF treatment could not be assessed because the drug was administered just after mosquito feeding on day two. The global infection, dissemination and transmission rates were quite low (4.09%, 1.91% and 0.54%, respectively); no mosquito was infected when viremia was below 1.26 × 10<sup>5</sup> RNA copies/mL. In conclusion, <i>Ae. aegypti</i> vector competence for ZIKV from macaques is low, likely to be due to low viral load and the short duration of ZIKV viremia in primates suitable for infecting susceptible mosquitoes. If ZIKV infection in human and macaques behaves similarly, transmission of the Zika virus in nature is most strongly affected by vector density. |
| first_indexed | 2024-03-10T14:38:01Z |
| format | Article |
| id | doaj.art-83afa5a7e2d04cce9ffbe86f549d180a |
| institution | Directory Open Access Journal |
| issn | 1999-4915 |
| language | English |
| last_indexed | 2024-03-10T14:38:01Z |
| publishDate | 2020-11-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Viruses |
| spelling | doaj.art-83afa5a7e2d04cce9ffbe86f549d180a2023-11-20T22:03:47ZengMDPI AGViruses1999-49152020-11-011212134510.3390/v12121345Low <i>Aedes aegypti</i> Vector Competence for Zika Virus from Viremic Rhesus MacaquesRosilainy Surubi Fernandes0Mariana Rocha David1Filipe Vieira Santos De Abreu2Anielly Ferreira-de-Brito3Noemi R. Gardinali4Sheila Maria Barbosa Lima5Márcia Cristina Ribeiro Andrade6Tatiana Kugelmeier7Jaqueline Mendes de Oliveira8Marcelo A. Pinto9Ricardo Lourenço-de-Oliveira10Laboratório de Mosquitos Transmissores de Hematozoários, Instituto Oswaldo Cruz, Fiocruz, Rio de Janeiro 21040-900, BrazilLaboratório de Mosquitos Transmissores de Hematozoários, Instituto Oswaldo Cruz, Fiocruz, Rio de Janeiro 21040-900, BrazilLaboratório de Mosquitos Transmissores de Hematozoários, Instituto Oswaldo Cruz, Fiocruz, Rio de Janeiro 21040-900, BrazilLaboratório de Mosquitos Transmissores de Hematozoários, Instituto Oswaldo Cruz, Fiocruz, Rio de Janeiro 21040-900, BrazilLaboratório de Desenvolvimento Tecnológico em Virologia, Instituto Oswaldo Cruz, Fiocruz, Rio de Janeiro 21040-900, BrazilLaboratório de Tecnologia Imunológica, Instituto de Tecnologia em Imunobiológicos Bio-Manguinhos, Fiocruz, Rio de Janeiro 21040-900, BrazilServiço de Criação de Primatas não humanos, Instituto de Ciência e Tecnologia em Biomodelos (ICTB), Fiocruz, Rio de Janeiro 21040-900, BrazilServiço de Criação de Primatas não humanos, Instituto de Ciência e Tecnologia em Biomodelos (ICTB), Fiocruz, Rio de Janeiro 21040-900, BrazilLaboratório de Desenvolvimento Tecnológico em Virologia, Instituto Oswaldo Cruz, Fiocruz, Rio de Janeiro 21040-900, BrazilLaboratório de Desenvolvimento Tecnológico em Virologia, Instituto Oswaldo Cruz, Fiocruz, Rio de Janeiro 21040-900, BrazilLaboratório de Mosquitos Transmissores de Hematozoários, Instituto Oswaldo Cruz, Fiocruz, Rio de Janeiro 21040-900, BrazilDespite worldwide efforts to understand the transmission dynamics of Zika virus (ZIKV), scanty evaluation has been made on the vector competence of <i>Aedes aegypti</i> fed directly on viremic human and non-human primates (NHPs). We blood-fed <i>Ae. aegypti</i> from two districts in Rio de Janeiro on six ZIKV infected pregnant rhesus macaques at several time points, half of which were treated with Sofosbuvir (SOF). Mosquitoes were analyzed for vector competence after 3, 7 and 14 days of incubation. Although viremia extended up to eight days post monkey inoculation, only mosquitoes fed on the day of the peak of viremia, recorded on day two, became infected. The influence of SOF treatment could not be assessed because the drug was administered just after mosquito feeding on day two. The global infection, dissemination and transmission rates were quite low (4.09%, 1.91% and 0.54%, respectively); no mosquito was infected when viremia was below 1.26 × 10<sup>5</sup> RNA copies/mL. In conclusion, <i>Ae. aegypti</i> vector competence for ZIKV from macaques is low, likely to be due to low viral load and the short duration of ZIKV viremia in primates suitable for infecting susceptible mosquitoes. If ZIKV infection in human and macaques behaves similarly, transmission of the Zika virus in nature is most strongly affected by vector density.https://www.mdpi.com/1999-4915/12/12/1345non-human primatesrhesus macaquesZikaviremiavectorial capacitytransmission |
| spellingShingle | Rosilainy Surubi Fernandes Mariana Rocha David Filipe Vieira Santos De Abreu Anielly Ferreira-de-Brito Noemi R. Gardinali Sheila Maria Barbosa Lima Márcia Cristina Ribeiro Andrade Tatiana Kugelmeier Jaqueline Mendes de Oliveira Marcelo A. Pinto Ricardo Lourenço-de-Oliveira Low <i>Aedes aegypti</i> Vector Competence for Zika Virus from Viremic Rhesus Macaques Viruses non-human primates rhesus macaques Zika viremia vectorial capacity transmission |
| title | Low <i>Aedes aegypti</i> Vector Competence for Zika Virus from Viremic Rhesus Macaques |
| title_full | Low <i>Aedes aegypti</i> Vector Competence for Zika Virus from Viremic Rhesus Macaques |
| title_fullStr | Low <i>Aedes aegypti</i> Vector Competence for Zika Virus from Viremic Rhesus Macaques |
| title_full_unstemmed | Low <i>Aedes aegypti</i> Vector Competence for Zika Virus from Viremic Rhesus Macaques |
| title_short | Low <i>Aedes aegypti</i> Vector Competence for Zika Virus from Viremic Rhesus Macaques |
| title_sort | low i aedes aegypti i vector competence for zika virus from viremic rhesus macaques |
| topic | non-human primates rhesus macaques Zika viremia vectorial capacity transmission |
| url | https://www.mdpi.com/1999-4915/12/12/1345 |
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