3,4-Dihydroxyphenylethanol (DPE or Hydroxytyrosol) Counteracts ERK1/2 and mTOR Activation, Pro-Inflammatory Cytokine Release, Autophagy and Mitophagy Reduction Mediated by Benzo[a]pyrene in Primary Human Colonic Epithelial Cells
Understanding the effects induced by carcinogens on primary colonic epithelial cells and how to counteract them might help to prevent colon cancer, which is one of the most frequent and aggressive cancers. In this study, we exposed primary human colonic epithelial cells (HCoEpC) to Benzo[a]pyrene (B...
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| Format: | Article |
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MDPI AG
2022-03-01
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| Series: | Pharmaceutics |
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| Online Access: | https://www.mdpi.com/1999-4923/14/3/663 |
| _version_ | 1797443568345808896 |
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| author | Roberta Santarelli Chiara Pompili Maria Saveria Gilardini Montani Lorenzo Evangelista Roberta Gonnella Mara Cirone |
| author_facet | Roberta Santarelli Chiara Pompili Maria Saveria Gilardini Montani Lorenzo Evangelista Roberta Gonnella Mara Cirone |
| author_sort | Roberta Santarelli |
| collection | DOAJ |
| description | Understanding the effects induced by carcinogens on primary colonic epithelial cells and how to counteract them might help to prevent colon cancer, which is one of the most frequent and aggressive cancers. In this study, we exposed primary human colonic epithelial cells (HCoEpC) to Benzo[a]pyrene (B[a]P) and found that it led to an increased production of pro-inflammatory cytokines and activated ERK1/2 and mTOR. These pathways are known to be involved in inflammatory bowel disease (IBD), which represents a colon cancer risk factor. Moreover, B[a]P reduced autophagy and mitophagy, processes whose dysregulation has been clearly demonstrated to predispose to cancer either by in vitro or in vivo studies. Interestingly, all the effects induced by B[a]P could be counteracted by 3,4-Dihydroxyphenylethanol (DPE or Hydroxytyrosol, H), the most powerful anti-inflammatory and antioxidant compound contained in olive oil. This study sheds light on the mechanisms that could be involved in colon carcinogenesis induced by a chemical carcinogen and identifies a safe natural product that may help to prevent them. |
| first_indexed | 2024-03-09T12:57:58Z |
| format | Article |
| id | doaj.art-83b283fb53ae4764a290979bd04386d0 |
| institution | Directory Open Access Journal |
| issn | 1999-4923 |
| language | English |
| last_indexed | 2024-03-09T12:57:58Z |
| publishDate | 2022-03-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Pharmaceutics |
| spelling | doaj.art-83b283fb53ae4764a290979bd04386d02023-11-30T21:58:08ZengMDPI AGPharmaceutics1999-49232022-03-0114366310.3390/pharmaceutics140306633,4-Dihydroxyphenylethanol (DPE or Hydroxytyrosol) Counteracts ERK1/2 and mTOR Activation, Pro-Inflammatory Cytokine Release, Autophagy and Mitophagy Reduction Mediated by Benzo[a]pyrene in Primary Human Colonic Epithelial CellsRoberta Santarelli0Chiara Pompili1Maria Saveria Gilardini Montani2Lorenzo Evangelista3Roberta Gonnella4Mara Cirone5Department of Experimental Medicine, “Sapienza” University of Rome, 00185 Rome, ItalyDepartment of Experimental Medicine, “Sapienza” University of Rome, 00185 Rome, ItalyDepartment of Experimental Medicine, “Sapienza” University of Rome, 00185 Rome, ItalyDepartment of Experimental Medicine, “Sapienza” University of Rome, 00185 Rome, ItalyDepartment of Experimental Medicine, “Sapienza” University of Rome, 00185 Rome, ItalyDepartment of Experimental Medicine, “Sapienza” University of Rome, 00185 Rome, ItalyUnderstanding the effects induced by carcinogens on primary colonic epithelial cells and how to counteract them might help to prevent colon cancer, which is one of the most frequent and aggressive cancers. In this study, we exposed primary human colonic epithelial cells (HCoEpC) to Benzo[a]pyrene (B[a]P) and found that it led to an increased production of pro-inflammatory cytokines and activated ERK1/2 and mTOR. These pathways are known to be involved in inflammatory bowel disease (IBD), which represents a colon cancer risk factor. Moreover, B[a]P reduced autophagy and mitophagy, processes whose dysregulation has been clearly demonstrated to predispose to cancer either by in vitro or in vivo studies. Interestingly, all the effects induced by B[a]P could be counteracted by 3,4-Dihydroxyphenylethanol (DPE or Hydroxytyrosol, H), the most powerful anti-inflammatory and antioxidant compound contained in olive oil. This study sheds light on the mechanisms that could be involved in colon carcinogenesis induced by a chemical carcinogen and identifies a safe natural product that may help to prevent them.https://www.mdpi.com/1999-4923/14/3/663Benzo[a]pyrene3,4-DihydroxyphenylethanolHydroxytyrosolhuman colonic epithelial cellsautophagyERK |
| spellingShingle | Roberta Santarelli Chiara Pompili Maria Saveria Gilardini Montani Lorenzo Evangelista Roberta Gonnella Mara Cirone 3,4-Dihydroxyphenylethanol (DPE or Hydroxytyrosol) Counteracts ERK1/2 and mTOR Activation, Pro-Inflammatory Cytokine Release, Autophagy and Mitophagy Reduction Mediated by Benzo[a]pyrene in Primary Human Colonic Epithelial Cells Pharmaceutics Benzo[a]pyrene 3,4-Dihydroxyphenylethanol Hydroxytyrosol human colonic epithelial cells autophagy ERK |
| title | 3,4-Dihydroxyphenylethanol (DPE or Hydroxytyrosol) Counteracts ERK1/2 and mTOR Activation, Pro-Inflammatory Cytokine Release, Autophagy and Mitophagy Reduction Mediated by Benzo[a]pyrene in Primary Human Colonic Epithelial Cells |
| title_full | 3,4-Dihydroxyphenylethanol (DPE or Hydroxytyrosol) Counteracts ERK1/2 and mTOR Activation, Pro-Inflammatory Cytokine Release, Autophagy and Mitophagy Reduction Mediated by Benzo[a]pyrene in Primary Human Colonic Epithelial Cells |
| title_fullStr | 3,4-Dihydroxyphenylethanol (DPE or Hydroxytyrosol) Counteracts ERK1/2 and mTOR Activation, Pro-Inflammatory Cytokine Release, Autophagy and Mitophagy Reduction Mediated by Benzo[a]pyrene in Primary Human Colonic Epithelial Cells |
| title_full_unstemmed | 3,4-Dihydroxyphenylethanol (DPE or Hydroxytyrosol) Counteracts ERK1/2 and mTOR Activation, Pro-Inflammatory Cytokine Release, Autophagy and Mitophagy Reduction Mediated by Benzo[a]pyrene in Primary Human Colonic Epithelial Cells |
| title_short | 3,4-Dihydroxyphenylethanol (DPE or Hydroxytyrosol) Counteracts ERK1/2 and mTOR Activation, Pro-Inflammatory Cytokine Release, Autophagy and Mitophagy Reduction Mediated by Benzo[a]pyrene in Primary Human Colonic Epithelial Cells |
| title_sort | 3 4 dihydroxyphenylethanol dpe or hydroxytyrosol counteracts erk1 2 and mtor activation pro inflammatory cytokine release autophagy and mitophagy reduction mediated by benzo a pyrene in primary human colonic epithelial cells |
| topic | Benzo[a]pyrene 3,4-Dihydroxyphenylethanol Hydroxytyrosol human colonic epithelial cells autophagy ERK |
| url | https://www.mdpi.com/1999-4923/14/3/663 |
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