Ultralow and Dynamic Flow Field Generator Composed of Microfluidic Peristaltic Pump
Precise control over flow rate and flow pattern is critical for investigating the contribution of shear force in cellular responses, biofluidic transport, and living matter in a flowing environment. Generation and control over the ultralow flow fields at microscale are, however, difficult, due to th...
| Main Authors: | , , , , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
Wiley
2023-05-01
|
| Series: | Advanced Intelligent Systems |
| Subjects: | |
| Online Access: | https://doi.org/10.1002/aisy.202200366 |
| _version_ | 1797823365643239424 |
|---|---|
| author | Bingchen Che Dan Sun Ningzhe Yan Wei Zhao Yonggang Liu Guangyin Jing Ce Zhang |
| author_facet | Bingchen Che Dan Sun Ningzhe Yan Wei Zhao Yonggang Liu Guangyin Jing Ce Zhang |
| author_sort | Bingchen Che |
| collection | DOAJ |
| description | Precise control over flow rate and flow pattern is critical for investigating the contribution of shear force in cellular responses, biofluidic transport, and living matter in a flowing environment. Generation and control over the ultralow flow fields at microscale are, however, difficult, due to thermal agitation and fluctuations in the surrounding environment. Herein, pa novel microfluidic flow field generator is proposed, capable of generating stable flow field down to the limit of thermal noise. Integrated with a customized MATLAB program controlling timely open–close of 18 monolithic PDMS valves, the flow generator outperforms the existing methods by both achievable low flow rate, that is, 0.01 nL s−1, temporal resolution of flow rate fluctuation down to 100 ms, and provides complex flow patterns. Moreover, unlike external devices (e.g., the syringe pump), the design can be easily integrated into any fluidic chip and sealed from the disturbance of experimental conditions, for example, the airflow and the movement of connected pipelines. The methodology is, therefore, suitable for biological and biophysical studies requiring a well‐defined flowing environment. |
| first_indexed | 2024-03-13T10:22:57Z |
| format | Article |
| id | doaj.art-83b3a8caa9664bb4b54eae2ee6054952 |
| institution | Directory Open Access Journal |
| issn | 2640-4567 |
| language | English |
| last_indexed | 2024-03-13T10:22:57Z |
| publishDate | 2023-05-01 |
| publisher | Wiley |
| record_format | Article |
| series | Advanced Intelligent Systems |
| spelling | doaj.art-83b3a8caa9664bb4b54eae2ee60549522023-05-20T03:54:51ZengWileyAdvanced Intelligent Systems2640-45672023-05-0155n/an/a10.1002/aisy.202200366Ultralow and Dynamic Flow Field Generator Composed of Microfluidic Peristaltic PumpBingchen Che0Dan Sun1Ningzhe Yan2Wei Zhao3Yonggang Liu4Guangyin Jing5Ce Zhang6State Key Laboratory of Photon-Technology in Western China Energy Institute of Photonics and Photon-Technology Northwest University Xi'an 710069 ChinaState Key Laboratory of Photon-Technology in Western China Energy Institute of Photonics and Photon-Technology Northwest University Xi'an 710069 ChinaSchool of Physics Northwest University Xi'an 710069 ChinaState Key Laboratory of Photon-Technology in Western China Energy Institute of Photonics and Photon-Technology Northwest University Xi'an 710069 ChinaLaboratory of Stem Cell and Tissue Engineering Chongqing Medical University ChongQing ChinaSchool of Physics Northwest University Xi'an 710069 ChinaState Key Laboratory of Photon-Technology in Western China Energy Institute of Photonics and Photon-Technology Northwest University Xi'an 710069 ChinaPrecise control over flow rate and flow pattern is critical for investigating the contribution of shear force in cellular responses, biofluidic transport, and living matter in a flowing environment. Generation and control over the ultralow flow fields at microscale are, however, difficult, due to thermal agitation and fluctuations in the surrounding environment. Herein, pa novel microfluidic flow field generator is proposed, capable of generating stable flow field down to the limit of thermal noise. Integrated with a customized MATLAB program controlling timely open–close of 18 monolithic PDMS valves, the flow generator outperforms the existing methods by both achievable low flow rate, that is, 0.01 nL s−1, temporal resolution of flow rate fluctuation down to 100 ms, and provides complex flow patterns. Moreover, unlike external devices (e.g., the syringe pump), the design can be easily integrated into any fluidic chip and sealed from the disturbance of experimental conditions, for example, the airflow and the movement of connected pipelines. The methodology is, therefore, suitable for biological and biophysical studies requiring a well‐defined flowing environment.https://doi.org/10.1002/aisy.202200366dynamic flowperistaltic pumpsultralow flow rates |
| spellingShingle | Bingchen Che Dan Sun Ningzhe Yan Wei Zhao Yonggang Liu Guangyin Jing Ce Zhang Ultralow and Dynamic Flow Field Generator Composed of Microfluidic Peristaltic Pump Advanced Intelligent Systems dynamic flow peristaltic pumps ultralow flow rates |
| title | Ultralow and Dynamic Flow Field Generator Composed of Microfluidic Peristaltic Pump |
| title_full | Ultralow and Dynamic Flow Field Generator Composed of Microfluidic Peristaltic Pump |
| title_fullStr | Ultralow and Dynamic Flow Field Generator Composed of Microfluidic Peristaltic Pump |
| title_full_unstemmed | Ultralow and Dynamic Flow Field Generator Composed of Microfluidic Peristaltic Pump |
| title_short | Ultralow and Dynamic Flow Field Generator Composed of Microfluidic Peristaltic Pump |
| title_sort | ultralow and dynamic flow field generator composed of microfluidic peristaltic pump |
| topic | dynamic flow peristaltic pumps ultralow flow rates |
| url | https://doi.org/10.1002/aisy.202200366 |
| work_keys_str_mv | AT bingchenche ultralowanddynamicflowfieldgeneratorcomposedofmicrofluidicperistalticpump AT dansun ultralowanddynamicflowfieldgeneratorcomposedofmicrofluidicperistalticpump AT ningzheyan ultralowanddynamicflowfieldgeneratorcomposedofmicrofluidicperistalticpump AT weizhao ultralowanddynamicflowfieldgeneratorcomposedofmicrofluidicperistalticpump AT yonggangliu ultralowanddynamicflowfieldgeneratorcomposedofmicrofluidicperistalticpump AT guangyinjing ultralowanddynamicflowfieldgeneratorcomposedofmicrofluidicperistalticpump AT cezhang ultralowanddynamicflowfieldgeneratorcomposedofmicrofluidicperistalticpump |