Homogeneous PLGA-lipid nanoparticle as a promising oral vaccine delivery system for ovalbumin
In this study, a polymeric lipid nanoparticle (NP) (simplified as Lipid NP) was reported as a promising oral vaccine delivery system. The Lipid NPs composed of a hydrophobic polymeric poly(d,l-lactide-co-glycolide) (PLGA) core and a surface coating of lipid monolayer. Membrane emulsification techniq...
| Main Authors: | , , , , |
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| Format: | Article |
| Language: | English |
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Elsevier
2014-06-01
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| Series: | Asian Journal of Pharmaceutical Sciences |
| Subjects: | |
| Online Access: | http://www.sciencedirect.com/science/article/pii/S1818087614000117 |
| _version_ | 1818887579231059968 |
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| author | Tongtong Ma Lianyan Wang Tingyuan Yang Guanghui Ma Siling Wang |
| author_facet | Tongtong Ma Lianyan Wang Tingyuan Yang Guanghui Ma Siling Wang |
| author_sort | Tongtong Ma |
| collection | DOAJ |
| description | In this study, a polymeric lipid nanoparticle (NP) (simplified as Lipid NP) was reported as a promising oral vaccine delivery system. The Lipid NPs composed of a hydrophobic polymeric poly(d,l-lactide-co-glycolide) (PLGA) core and a surface coating of lipid monolayer. Membrane emulsification technique was used to obtain uniform-sized Lipid NPs. Ovalbumin (OVA) was used as a model vaccine. Compared with the pure PLGA NPs, the Lipid NPs achieved higher loading capacity (LC) and entrapment efficiency (EE) for the encapsulated OVA. An in vitro oral release profile showed that the OVA-Lipid NPs were with lower initial burst and could protect the loaded OVA from the harsh gastrointestinal (GI) environment for a long time. In addition, a human microfold cell (M-cell) transcytotic assay demonstrated that due to a lipid layer structure on the particle surface, the Lipid NPs showed higher affinity to the M-cells. Since the M-cell in the intestinal epithelium played an important role in particle transportation as well as intimately associated with the underlying immune cells, the OVA-Lipid NPs effectively induced mucosal and humoral immune responses. |
| first_indexed | 2024-12-19T16:39:28Z |
| format | Article |
| id | doaj.art-83b714c9bfe54f79b8a680a047754a1f |
| institution | Directory Open Access Journal |
| issn | 1818-0876 |
| language | English |
| last_indexed | 2024-12-19T16:39:28Z |
| publishDate | 2014-06-01 |
| publisher | Elsevier |
| record_format | Article |
| series | Asian Journal of Pharmaceutical Sciences |
| spelling | doaj.art-83b714c9bfe54f79b8a680a047754a1f2022-12-21T20:13:51ZengElsevierAsian Journal of Pharmaceutical Sciences1818-08762014-06-019312913610.1016/j.ajps.2014.03.002Homogeneous PLGA-lipid nanoparticle as a promising oral vaccine delivery system for ovalbuminTongtong Ma0Lianyan Wang1Tingyuan Yang2Guanghui Ma3Siling Wang4Department of Pharmaceutics, Shenyang Pharmaceutical University, Shenyang, PR ChinaNational Key Laboratory of Biochemical Engineering, PLA Key Laboratory of Biopharmaceutical Production & Formulation Engineering, Institute of Process Engineering, Chinese Academy of Sciences, Beijing, PR ChinaNational Key Laboratory of Biochemical Engineering, PLA Key Laboratory of Biopharmaceutical Production & Formulation Engineering, Institute of Process Engineering, Chinese Academy of Sciences, Beijing, PR ChinaNational Key Laboratory of Biochemical Engineering, PLA Key Laboratory of Biopharmaceutical Production & Formulation Engineering, Institute of Process Engineering, Chinese Academy of Sciences, Beijing, PR ChinaDepartment of Pharmaceutics, Shenyang Pharmaceutical University, Shenyang, PR ChinaIn this study, a polymeric lipid nanoparticle (NP) (simplified as Lipid NP) was reported as a promising oral vaccine delivery system. The Lipid NPs composed of a hydrophobic polymeric poly(d,l-lactide-co-glycolide) (PLGA) core and a surface coating of lipid monolayer. Membrane emulsification technique was used to obtain uniform-sized Lipid NPs. Ovalbumin (OVA) was used as a model vaccine. Compared with the pure PLGA NPs, the Lipid NPs achieved higher loading capacity (LC) and entrapment efficiency (EE) for the encapsulated OVA. An in vitro oral release profile showed that the OVA-Lipid NPs were with lower initial burst and could protect the loaded OVA from the harsh gastrointestinal (GI) environment for a long time. In addition, a human microfold cell (M-cell) transcytotic assay demonstrated that due to a lipid layer structure on the particle surface, the Lipid NPs showed higher affinity to the M-cells. Since the M-cell in the intestinal epithelium played an important role in particle transportation as well as intimately associated with the underlying immune cells, the OVA-Lipid NPs effectively induced mucosal and humoral immune responses.http://www.sciencedirect.com/science/article/pii/S1818087614000117Oral deliveryVaccinePolymeric lipid nanoparticleM-cell affinity |
| spellingShingle | Tongtong Ma Lianyan Wang Tingyuan Yang Guanghui Ma Siling Wang Homogeneous PLGA-lipid nanoparticle as a promising oral vaccine delivery system for ovalbumin Asian Journal of Pharmaceutical Sciences Oral delivery Vaccine Polymeric lipid nanoparticle M-cell affinity |
| title | Homogeneous PLGA-lipid nanoparticle as a promising oral vaccine delivery system for ovalbumin |
| title_full | Homogeneous PLGA-lipid nanoparticle as a promising oral vaccine delivery system for ovalbumin |
| title_fullStr | Homogeneous PLGA-lipid nanoparticle as a promising oral vaccine delivery system for ovalbumin |
| title_full_unstemmed | Homogeneous PLGA-lipid nanoparticle as a promising oral vaccine delivery system for ovalbumin |
| title_short | Homogeneous PLGA-lipid nanoparticle as a promising oral vaccine delivery system for ovalbumin |
| title_sort | homogeneous plga lipid nanoparticle as a promising oral vaccine delivery system for ovalbumin |
| topic | Oral delivery Vaccine Polymeric lipid nanoparticle M-cell affinity |
| url | http://www.sciencedirect.com/science/article/pii/S1818087614000117 |
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