Complete Chloroplast Genome of the Wollemi Pine (Wollemia nobilis): Structure and Evolution.
The Wollemi pine (Wollemia nobilis) is a rare Southern conifer with striking morphological similarity to fossil pines. A small population of W. nobilis was discovered in 1994 in a remote canyon system in the Wollemi National Park (near Sydney, Australia). This population contains fewer than 100 indi...
| Main Authors: | , , , , , , , , , , , , |
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| Format: | Article |
| Language: | English |
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Public Library of Science (PLoS)
2015-01-01
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| Series: | PLoS ONE |
| Online Access: | http://europepmc.org/articles/PMC4464890?pdf=render |
| _version_ | 1818501568566132736 |
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| author | Jia-Yee S Yap Thore Rohner Abigail Greenfield Marlien Van Der Merwe Hannah McPherson Wendy Glenn Geoff Kornfeld Elessa Marendy Annie Y H Pan Alan Wilton Marc R Wilkins Maurizio Rossetto Sven K Delaney |
| author_facet | Jia-Yee S Yap Thore Rohner Abigail Greenfield Marlien Van Der Merwe Hannah McPherson Wendy Glenn Geoff Kornfeld Elessa Marendy Annie Y H Pan Alan Wilton Marc R Wilkins Maurizio Rossetto Sven K Delaney |
| author_sort | Jia-Yee S Yap |
| collection | DOAJ |
| description | The Wollemi pine (Wollemia nobilis) is a rare Southern conifer with striking morphological similarity to fossil pines. A small population of W. nobilis was discovered in 1994 in a remote canyon system in the Wollemi National Park (near Sydney, Australia). This population contains fewer than 100 individuals and is critically endangered. Previous genetic studies of the Wollemi pine have investigated its evolutionary relationship with other pines in the family Araucariaceae, and have suggested that the Wollemi pine genome contains little or no variation. However, these studies were performed prior to the widespread use of genome sequencing, and their conclusions were based on a limited fraction of the Wollemi pine genome. In this study, we address this problem by determining the entire sequence of the W. nobilis chloroplast genome. A detailed analysis of the structure of the genome is presented, and the evolution of the genome is inferred by comparison with the chloroplast sequences of other members of the Araucariaceae and the related family Podocarpaceae. Pairwise alignments of whole genome sequences, and the presence of unique pseudogenes, gene duplications and insertions in W. nobilis and Araucariaceae, indicate that the W. nobilis chloroplast genome is most similar to that of its sister taxon Agathis. However, the W. nobilis genome contains an unusually high number of repetitive sequences, and these could be used in future studies to investigate and conserve any remnant genetic diversity in the Wollemi pine. |
| first_indexed | 2024-12-10T20:58:05Z |
| format | Article |
| id | doaj.art-83b89a265a3b4dfebc2a490ef2953f85 |
| institution | Directory Open Access Journal |
| issn | 1932-6203 |
| language | English |
| last_indexed | 2024-12-10T20:58:05Z |
| publishDate | 2015-01-01 |
| publisher | Public Library of Science (PLoS) |
| record_format | Article |
| series | PLoS ONE |
| spelling | doaj.art-83b89a265a3b4dfebc2a490ef2953f852022-12-22T01:33:54ZengPublic Library of Science (PLoS)PLoS ONE1932-62032015-01-01106e012812610.1371/journal.pone.0128126Complete Chloroplast Genome of the Wollemi Pine (Wollemia nobilis): Structure and Evolution.Jia-Yee S YapThore RohnerAbigail GreenfieldMarlien Van Der MerweHannah McPhersonWendy GlennGeoff KornfeldElessa MarendyAnnie Y H PanAlan WiltonMarc R WilkinsMaurizio RossettoSven K DelaneyThe Wollemi pine (Wollemia nobilis) is a rare Southern conifer with striking morphological similarity to fossil pines. A small population of W. nobilis was discovered in 1994 in a remote canyon system in the Wollemi National Park (near Sydney, Australia). This population contains fewer than 100 individuals and is critically endangered. Previous genetic studies of the Wollemi pine have investigated its evolutionary relationship with other pines in the family Araucariaceae, and have suggested that the Wollemi pine genome contains little or no variation. However, these studies were performed prior to the widespread use of genome sequencing, and their conclusions were based on a limited fraction of the Wollemi pine genome. In this study, we address this problem by determining the entire sequence of the W. nobilis chloroplast genome. A detailed analysis of the structure of the genome is presented, and the evolution of the genome is inferred by comparison with the chloroplast sequences of other members of the Araucariaceae and the related family Podocarpaceae. Pairwise alignments of whole genome sequences, and the presence of unique pseudogenes, gene duplications and insertions in W. nobilis and Araucariaceae, indicate that the W. nobilis chloroplast genome is most similar to that of its sister taxon Agathis. However, the W. nobilis genome contains an unusually high number of repetitive sequences, and these could be used in future studies to investigate and conserve any remnant genetic diversity in the Wollemi pine.http://europepmc.org/articles/PMC4464890?pdf=render |
| spellingShingle | Jia-Yee S Yap Thore Rohner Abigail Greenfield Marlien Van Der Merwe Hannah McPherson Wendy Glenn Geoff Kornfeld Elessa Marendy Annie Y H Pan Alan Wilton Marc R Wilkins Maurizio Rossetto Sven K Delaney Complete Chloroplast Genome of the Wollemi Pine (Wollemia nobilis): Structure and Evolution. PLoS ONE |
| title | Complete Chloroplast Genome of the Wollemi Pine (Wollemia nobilis): Structure and Evolution. |
| title_full | Complete Chloroplast Genome of the Wollemi Pine (Wollemia nobilis): Structure and Evolution. |
| title_fullStr | Complete Chloroplast Genome of the Wollemi Pine (Wollemia nobilis): Structure and Evolution. |
| title_full_unstemmed | Complete Chloroplast Genome of the Wollemi Pine (Wollemia nobilis): Structure and Evolution. |
| title_short | Complete Chloroplast Genome of the Wollemi Pine (Wollemia nobilis): Structure and Evolution. |
| title_sort | complete chloroplast genome of the wollemi pine wollemia nobilis structure and evolution |
| url | http://europepmc.org/articles/PMC4464890?pdf=render |
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