Whole-genome sequencing of a Plasmodium vivax isolate from the China-Myanmar border area
Currently, there is a trend of an increasing number of Plasmodium vivaxmalaria cases in China that are imported across its Southeast Asia border, especially in the China-Myanmar border area (CMB). To date, little is known about the genetic diversity of P. vivaxin this region. In this paper, we repor...
| Main Authors: | , , , |
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| Format: | Article |
| Language: | English |
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Fundação Oswaldo Cruz (FIOCRUZ)
2015-09-01
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| Series: | Memorias do Instituto Oswaldo Cruz |
| Subjects: | |
| Online Access: | http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0074-02762015000600814&lng=en&tlng=en |
| _version_ | 1827839712760430592 |
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| author | Hai-Mo Shen Shen-Bo Chen Yue Wang Jun-Hu Chen |
| author_facet | Hai-Mo Shen Shen-Bo Chen Yue Wang Jun-Hu Chen |
| author_sort | Hai-Mo Shen |
| collection | DOAJ |
| description | Currently, there is a trend of an increasing number of Plasmodium vivaxmalaria cases in China that are imported across its Southeast Asia border, especially in the China-Myanmar border area (CMB). To date, little is known about the genetic diversity of P. vivaxin this region. In this paper, we report the first genome sequencing of a P. vivaxisolate (CMB-1) from a vivax malaria patient in CMB. The sequencing data were aligned onto 96.43% of the P. vivaxSalvador I reference strain (Sal I) genome with 7.84-fold coverage as well as onto 98.32% of 14 Sal I chromosomes. Using the de novoassembly approach, we generated 8,541 scaffolds and assembled a total of 27.1 Mb of sequence into CMB-1 scaffolds. Furthermore, we identified all 295 known virgenes, which is the largest subtelomeric multigene family in malaria parasites. These results provide an important foundation for further research onP. vivaxpopulation genetics. |
| first_indexed | 2024-03-12T07:23:20Z |
| format | Article |
| id | doaj.art-83b985f91111436d85e765e6ece6de57 |
| institution | Directory Open Access Journal |
| issn | 1678-8060 |
| language | English |
| last_indexed | 2024-03-12T07:23:20Z |
| publishDate | 2015-09-01 |
| publisher | Fundação Oswaldo Cruz (FIOCRUZ) |
| record_format | Article |
| series | Memorias do Instituto Oswaldo Cruz |
| spelling | doaj.art-83b985f91111436d85e765e6ece6de572023-09-02T22:14:56ZengFundação Oswaldo Cruz (FIOCRUZ)Memorias do Instituto Oswaldo Cruz1678-80602015-09-01110681481610.1590/0074-02760150216S0074-02762015000600814Whole-genome sequencing of a Plasmodium vivax isolate from the China-Myanmar border areaHai-Mo ShenShen-Bo ChenYue WangJun-Hu ChenCurrently, there is a trend of an increasing number of Plasmodium vivaxmalaria cases in China that are imported across its Southeast Asia border, especially in the China-Myanmar border area (CMB). To date, little is known about the genetic diversity of P. vivaxin this region. In this paper, we report the first genome sequencing of a P. vivaxisolate (CMB-1) from a vivax malaria patient in CMB. The sequencing data were aligned onto 96.43% of the P. vivaxSalvador I reference strain (Sal I) genome with 7.84-fold coverage as well as onto 98.32% of 14 Sal I chromosomes. Using the de novoassembly approach, we generated 8,541 scaffolds and assembled a total of 27.1 Mb of sequence into CMB-1 scaffolds. Furthermore, we identified all 295 known virgenes, which is the largest subtelomeric multigene family in malaria parasites. These results provide an important foundation for further research onP. vivaxpopulation genetics.http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0074-02762015000600814&lng=en&tlng=enPlasmodium vivaxgenomevir |
| spellingShingle | Hai-Mo Shen Shen-Bo Chen Yue Wang Jun-Hu Chen Whole-genome sequencing of a Plasmodium vivax isolate from the China-Myanmar border area Memorias do Instituto Oswaldo Cruz Plasmodium vivax genome vir |
| title | Whole-genome sequencing of a Plasmodium vivax isolate from the China-Myanmar border area |
| title_full | Whole-genome sequencing of a Plasmodium vivax isolate from the China-Myanmar border area |
| title_fullStr | Whole-genome sequencing of a Plasmodium vivax isolate from the China-Myanmar border area |
| title_full_unstemmed | Whole-genome sequencing of a Plasmodium vivax isolate from the China-Myanmar border area |
| title_short | Whole-genome sequencing of a Plasmodium vivax isolate from the China-Myanmar border area |
| title_sort | whole genome sequencing of a plasmodium vivax isolate from the china myanmar border area |
| topic | Plasmodium vivax genome vir |
| url | http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0074-02762015000600814&lng=en&tlng=en |
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