Osteoclast-Driven Osteogenesis, Bone Remodeling and Biomaterial Resorption: A New Profile of BMP2-CPC-Induced Alveolar Bone Regeneration
This bedside-to-bench study aimed to systematically investigate the value of applying BMP2-loaded calcium phosphate cement (BMP2-CPC) in the restoration of large-scale alveolar bone defects. Compared to deproteinized bovine bone (DBB), BMP2-CPC was shown to be capable of inducing a favorable pattern...
| Main Authors: | , , , , , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
MDPI AG
2022-10-01
|
| Series: | International Journal of Molecular Sciences |
| Subjects: | |
| Online Access: | https://www.mdpi.com/1422-0067/23/20/12204 |
| _version_ | 1797472840191049728 |
|---|---|
| author | Hongzhou Shen Yu Zhuang Chenglong Zhang Changru Zhang Yuan Yuan Hongbo Yu Jiawen Si Guofang Shen |
| author_facet | Hongzhou Shen Yu Zhuang Chenglong Zhang Changru Zhang Yuan Yuan Hongbo Yu Jiawen Si Guofang Shen |
| author_sort | Hongzhou Shen |
| collection | DOAJ |
| description | This bedside-to-bench study aimed to systematically investigate the value of applying BMP2-loaded calcium phosphate cement (BMP2-CPC) in the restoration of large-scale alveolar bone defects. Compared to deproteinized bovine bone (DBB), BMP2-CPC was shown to be capable of inducing a favorable pattern of bone regeneration and bone remodeling accompanied by active osteoclastogenesis and optimized biomaterial resorption when applied in reconstructive periodontally accelerated osteogenic orthodontics (PAOO) surgery. To verify the regulatory role of osteoclasts in the BMP2-CPC-induced pattern of bone regeneration, in vitro and in vivo studies were designed to elucidate the underlying mechanism. Our results revealed that osteoclasts played a multifaceted role (facilitating osteogenesis, bone remodeling and biomaterial resorption) in the BMP2-CPC-induced bone regeneration. Osteoclasts contributed to the osteogenic differentiation of mesenchymal stem cells (MSCs) by secreting calcium ions, CTHRC1 and PDGF-B. Moreover, the increased osteoclasts promoted the remodeling of new bone and BMP2-CPC resorption, leading to a harmonized replacement of biomaterials with mature bone. In conclusion, the in vitro and in vivo experimental results corresponded with the clinical results and showed the optimized properties of BMP2-CPC in activating osteoclast-driven bone regeneration and remodeling, thus indicating the highly promising prospects of BMP2-CPC as an ideal therapeutic for alveolar bone defects. |
| first_indexed | 2024-03-09T20:06:51Z |
| format | Article |
| id | doaj.art-83ba9fe4797a438c98750459e3d988b0 |
| institution | Directory Open Access Journal |
| issn | 1661-6596 1422-0067 |
| language | English |
| last_indexed | 2024-03-09T20:06:51Z |
| publishDate | 2022-10-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | International Journal of Molecular Sciences |
| spelling | doaj.art-83ba9fe4797a438c98750459e3d988b02023-11-24T00:29:52ZengMDPI AGInternational Journal of Molecular Sciences1661-65961422-00672022-10-0123201220410.3390/ijms232012204Osteoclast-Driven Osteogenesis, Bone Remodeling and Biomaterial Resorption: A New Profile of BMP2-CPC-Induced Alveolar Bone RegenerationHongzhou Shen0Yu Zhuang1Chenglong Zhang2Changru Zhang3Yuan Yuan4Hongbo Yu5Jiawen Si6Guofang Shen7Department of Oral and Craniomaxillofacial Surgery, Shanghai Ninth People’s Hospital, College of Stomatology, Shanghai Jiao Tong University School of Medicine, Shanghai 200011, ChinaDepartment of Oral and Craniomaxillofacial Surgery, Shanghai Ninth People’s Hospital, College of Stomatology, Shanghai Jiao Tong University School of Medicine, Shanghai 200011, ChinaDepartment of Oral and Craniomaxillofacial Surgery, Shanghai Ninth People’s Hospital, College of Stomatology, Shanghai Jiao Tong University School of Medicine, Shanghai 200011, ChinaKey Laboratory for Ultrafine Materials of Ministry of Education, East China University of Science and Technology, Shanghai 200237, ChinaKey Laboratory for Ultrafine Materials of Ministry of Education, East China University of Science and Technology, Shanghai 200237, ChinaDepartment of Oral and Craniomaxillofacial Surgery, Shanghai Ninth People’s Hospital, College of Stomatology, Shanghai Jiao Tong University School of Medicine, Shanghai 200011, ChinaDepartment of Oral and Craniomaxillofacial Surgery, Shanghai Ninth People’s Hospital, College of Stomatology, Shanghai Jiao Tong University School of Medicine, Shanghai 200011, ChinaDepartment of Oral and Craniomaxillofacial Surgery, Shanghai Ninth People’s Hospital, College of Stomatology, Shanghai Jiao Tong University School of Medicine, Shanghai 200011, ChinaThis bedside-to-bench study aimed to systematically investigate the value of applying BMP2-loaded calcium phosphate cement (BMP2-CPC) in the restoration of large-scale alveolar bone defects. Compared to deproteinized bovine bone (DBB), BMP2-CPC was shown to be capable of inducing a favorable pattern of bone regeneration and bone remodeling accompanied by active osteoclastogenesis and optimized biomaterial resorption when applied in reconstructive periodontally accelerated osteogenic orthodontics (PAOO) surgery. To verify the regulatory role of osteoclasts in the BMP2-CPC-induced pattern of bone regeneration, in vitro and in vivo studies were designed to elucidate the underlying mechanism. Our results revealed that osteoclasts played a multifaceted role (facilitating osteogenesis, bone remodeling and biomaterial resorption) in the BMP2-CPC-induced bone regeneration. Osteoclasts contributed to the osteogenic differentiation of mesenchymal stem cells (MSCs) by secreting calcium ions, CTHRC1 and PDGF-B. Moreover, the increased osteoclasts promoted the remodeling of new bone and BMP2-CPC resorption, leading to a harmonized replacement of biomaterials with mature bone. In conclusion, the in vitro and in vivo experimental results corresponded with the clinical results and showed the optimized properties of BMP2-CPC in activating osteoclast-driven bone regeneration and remodeling, thus indicating the highly promising prospects of BMP2-CPC as an ideal therapeutic for alveolar bone defects.https://www.mdpi.com/1422-0067/23/20/12204osteoclastsalveolar bone regenerationbone remodelingbiomaterial resorptioncalcium phosphate cement |
| spellingShingle | Hongzhou Shen Yu Zhuang Chenglong Zhang Changru Zhang Yuan Yuan Hongbo Yu Jiawen Si Guofang Shen Osteoclast-Driven Osteogenesis, Bone Remodeling and Biomaterial Resorption: A New Profile of BMP2-CPC-Induced Alveolar Bone Regeneration International Journal of Molecular Sciences osteoclasts alveolar bone regeneration bone remodeling biomaterial resorption calcium phosphate cement |
| title | Osteoclast-Driven Osteogenesis, Bone Remodeling and Biomaterial Resorption: A New Profile of BMP2-CPC-Induced Alveolar Bone Regeneration |
| title_full | Osteoclast-Driven Osteogenesis, Bone Remodeling and Biomaterial Resorption: A New Profile of BMP2-CPC-Induced Alveolar Bone Regeneration |
| title_fullStr | Osteoclast-Driven Osteogenesis, Bone Remodeling and Biomaterial Resorption: A New Profile of BMP2-CPC-Induced Alveolar Bone Regeneration |
| title_full_unstemmed | Osteoclast-Driven Osteogenesis, Bone Remodeling and Biomaterial Resorption: A New Profile of BMP2-CPC-Induced Alveolar Bone Regeneration |
| title_short | Osteoclast-Driven Osteogenesis, Bone Remodeling and Biomaterial Resorption: A New Profile of BMP2-CPC-Induced Alveolar Bone Regeneration |
| title_sort | osteoclast driven osteogenesis bone remodeling and biomaterial resorption a new profile of bmp2 cpc induced alveolar bone regeneration |
| topic | osteoclasts alveolar bone regeneration bone remodeling biomaterial resorption calcium phosphate cement |
| url | https://www.mdpi.com/1422-0067/23/20/12204 |
| work_keys_str_mv | AT hongzhoushen osteoclastdrivenosteogenesisboneremodelingandbiomaterialresorptionanewprofileofbmp2cpcinducedalveolarboneregeneration AT yuzhuang osteoclastdrivenosteogenesisboneremodelingandbiomaterialresorptionanewprofileofbmp2cpcinducedalveolarboneregeneration AT chenglongzhang osteoclastdrivenosteogenesisboneremodelingandbiomaterialresorptionanewprofileofbmp2cpcinducedalveolarboneregeneration AT changruzhang osteoclastdrivenosteogenesisboneremodelingandbiomaterialresorptionanewprofileofbmp2cpcinducedalveolarboneregeneration AT yuanyuan osteoclastdrivenosteogenesisboneremodelingandbiomaterialresorptionanewprofileofbmp2cpcinducedalveolarboneregeneration AT hongboyu osteoclastdrivenosteogenesisboneremodelingandbiomaterialresorptionanewprofileofbmp2cpcinducedalveolarboneregeneration AT jiawensi osteoclastdrivenosteogenesisboneremodelingandbiomaterialresorptionanewprofileofbmp2cpcinducedalveolarboneregeneration AT guofangshen osteoclastdrivenosteogenesisboneremodelingandbiomaterialresorptionanewprofileofbmp2cpcinducedalveolarboneregeneration |