Lower expression of NOTCH components in peripheral blood mononuclear cells of allogeneic hematopoietic cell transplant patients
Introduction: Chronic graft-versus-host disease (cGvHD) not only remains the main cause of late mortality after allogeneic hematopoietic cell transplant, but also has the capacity of causing severe organ impairment in those who survive. The Notch, a highly conserved ligand-receptor pathway, is invol...
| Main Authors: | , , , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
Elsevier
2023-07-01
|
| Series: | Hematology, Transfusion and Cell Therapy |
| Subjects: | |
| Online Access: | http://www.sciencedirect.com/science/article/pii/S2531137922000864 |
| _version_ | 1797732867302752256 |
|---|---|
| author | Marcos Paulo Colella Beatriz Corey Morini Fernanda Niemann Matheus Rodrigues Lopes Sara Olalla Saad Patricia Favaro |
| author_facet | Marcos Paulo Colella Beatriz Corey Morini Fernanda Niemann Matheus Rodrigues Lopes Sara Olalla Saad Patricia Favaro |
| author_sort | Marcos Paulo Colella |
| collection | DOAJ |
| description | Introduction: Chronic graft-versus-host disease (cGvHD) not only remains the main cause of late mortality after allogeneic hematopoietic cell transplant, but also has the capacity of causing severe organ impairment in those who survive. The Notch, a highly conserved ligand-receptor pathway, is involved in many immunological processes, including inflammatory and regulatory responses. Recently, mouse models have shown that the blockage of canonical Notch signaling prevents GvHD. Objective and Method: Due to the lack of data on the Notch pathway in human chronic GvHD, we sought to study the expression of NOTCH components in primary samples of patients who received allo-HCT and presented active cGvHD or a long-term clinical tolerance to cGvHD. Results: Our results showed a significantly lower expression of NOTCH components in both groups that received allo-HCT, independently of their cGvHD status, when compared to healthy controls. Conclusion: Moreover, there were no differences in gene expression levels between the active cGvHD and clinically tolerant groups. To our knowledge, this is one of the first studies performed in human primary samples and our data indicate that much remains to be learned regarding NOTCH signaling as a new regulator of GvHD. |
| first_indexed | 2024-03-12T12:20:42Z |
| format | Article |
| id | doaj.art-83bae80c4f164f57882a2990f27f88f9 |
| institution | Directory Open Access Journal |
| issn | 2531-1379 |
| language | English |
| last_indexed | 2024-03-12T12:20:42Z |
| publishDate | 2023-07-01 |
| publisher | Elsevier |
| record_format | Article |
| series | Hematology, Transfusion and Cell Therapy |
| spelling | doaj.art-83bae80c4f164f57882a2990f27f88f92023-08-30T05:54:32ZengElsevierHematology, Transfusion and Cell Therapy2531-13792023-07-01453324329Lower expression of NOTCH components in peripheral blood mononuclear cells of allogeneic hematopoietic cell transplant patientsMarcos Paulo Colella0Beatriz Corey Morini1Fernanda Niemann2Matheus Rodrigues Lopes3Sara Olalla Saad4Patricia Favaro5Universidade Estadual de Campinas (Unicamp), Campinas, SP, BrazilUniversidade Estadual de Campinas (Unicamp), Campinas, SP, BrazilUniversidade Estadual de Campinas (Unicamp), Campinas, SP, BrazilUniversidade Federal do Vale do São Francisco (Univasf), Paulo Afonso, BA, BrazilUniversidade Estadual de Campinas (Unicamp), Campinas, SP, BrazilUniversidade Estadual de Campinas (Unicamp), Campinas, SP, Brazil; Universidade Federal de São Paulo, (Unifesp), Diadema, SP, Brazil; Corresponding author at: Universidade Federal de São Paulo (UNIFESP), Rua São Nicolau, 210, Diadema, SP, CEP 09913-030, BrazilIntroduction: Chronic graft-versus-host disease (cGvHD) not only remains the main cause of late mortality after allogeneic hematopoietic cell transplant, but also has the capacity of causing severe organ impairment in those who survive. The Notch, a highly conserved ligand-receptor pathway, is involved in many immunological processes, including inflammatory and regulatory responses. Recently, mouse models have shown that the blockage of canonical Notch signaling prevents GvHD. Objective and Method: Due to the lack of data on the Notch pathway in human chronic GvHD, we sought to study the expression of NOTCH components in primary samples of patients who received allo-HCT and presented active cGvHD or a long-term clinical tolerance to cGvHD. Results: Our results showed a significantly lower expression of NOTCH components in both groups that received allo-HCT, independently of their cGvHD status, when compared to healthy controls. Conclusion: Moreover, there were no differences in gene expression levels between the active cGvHD and clinically tolerant groups. To our knowledge, this is one of the first studies performed in human primary samples and our data indicate that much remains to be learned regarding NOTCH signaling as a new regulator of GvHD.http://www.sciencedirect.com/science/article/pii/S2531137922000864NotchChronic graft-versus-host-diseaseAllogeneic hematopoietic cell transplantTolerance |
| spellingShingle | Marcos Paulo Colella Beatriz Corey Morini Fernanda Niemann Matheus Rodrigues Lopes Sara Olalla Saad Patricia Favaro Lower expression of NOTCH components in peripheral blood mononuclear cells of allogeneic hematopoietic cell transplant patients Hematology, Transfusion and Cell Therapy Notch Chronic graft-versus-host-disease Allogeneic hematopoietic cell transplant Tolerance |
| title | Lower expression of NOTCH components in peripheral blood mononuclear cells of allogeneic hematopoietic cell transplant patients |
| title_full | Lower expression of NOTCH components in peripheral blood mononuclear cells of allogeneic hematopoietic cell transplant patients |
| title_fullStr | Lower expression of NOTCH components in peripheral blood mononuclear cells of allogeneic hematopoietic cell transplant patients |
| title_full_unstemmed | Lower expression of NOTCH components in peripheral blood mononuclear cells of allogeneic hematopoietic cell transplant patients |
| title_short | Lower expression of NOTCH components in peripheral blood mononuclear cells of allogeneic hematopoietic cell transplant patients |
| title_sort | lower expression of notch components in peripheral blood mononuclear cells of allogeneic hematopoietic cell transplant patients |
| topic | Notch Chronic graft-versus-host-disease Allogeneic hematopoietic cell transplant Tolerance |
| url | http://www.sciencedirect.com/science/article/pii/S2531137922000864 |
| work_keys_str_mv | AT marcospaulocolella lowerexpressionofnotchcomponentsinperipheralbloodmononuclearcellsofallogeneichematopoieticcelltransplantpatients AT beatrizcoreymorini lowerexpressionofnotchcomponentsinperipheralbloodmononuclearcellsofallogeneichematopoieticcelltransplantpatients AT fernandaniemann lowerexpressionofnotchcomponentsinperipheralbloodmononuclearcellsofallogeneichematopoieticcelltransplantpatients AT matheusrodrigueslopes lowerexpressionofnotchcomponentsinperipheralbloodmononuclearcellsofallogeneichematopoieticcelltransplantpatients AT saraolallasaad lowerexpressionofnotchcomponentsinperipheralbloodmononuclearcellsofallogeneichematopoieticcelltransplantpatients AT patriciafavaro lowerexpressionofnotchcomponentsinperipheralbloodmononuclearcellsofallogeneichematopoieticcelltransplantpatients |