Expression of Oxytocin/Neurophysin I and Oxytocinase in the Equine Conceptus from Day 8 to Day 21 Post-Ovulation
Leucyl and cystinyl aminopeptidase (LNPEP/oxytocinase) is an enzyme that metabolizes oxytocin in serum and tissues. The presence of oxytocin/neurophysin I (OXT), oxytocin and LNPEP and their relationship to other genes is unknown in the equine conceptus. Our objective was to characterize gene expres...
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MDPI AG
2022-03-01
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| Online Access: | https://www.mdpi.com/2076-2615/12/7/799 |
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| author | Mariana Diel de Amorim Claudia Klein Robert Foster Lynn Dong Maria Fernanda Lopez-Rodriguez Claire Card |
| author_facet | Mariana Diel de Amorim Claudia Klein Robert Foster Lynn Dong Maria Fernanda Lopez-Rodriguez Claire Card |
| author_sort | Mariana Diel de Amorim |
| collection | DOAJ |
| description | Leucyl and cystinyl aminopeptidase (LNPEP/oxytocinase) is an enzyme that metabolizes oxytocin in serum and tissues. The presence of oxytocin/neurophysin I (OXT), oxytocin and LNPEP and their relationship to other genes is unknown in the equine conceptus. Our objective was to characterize gene expression of LNPEP and OXT on D8, 10, 12, 14, 15, 16 and 21 conceptuses in relationship to other genes. Immunohistochemistry, western blot and liquid chromatography with tandem mass spectrometry (LC-MS/MS) were used for identification of oxytocin and LNPEP in D15, 16 and 18 conceptuses. LNPEP was increased at D15 compared to D10, was immunolocalized in the equine trophectoderm and endoderm, and protein was confirmed by LC-MS/MS. Maximal abundance of OXT was at D21, and lowest on D12 and D14, but no protein was identified. OXTR abundance was highest on D14 and D21. LNPEP was correlated with PTGFR and PTGES on D12 and D14–D15, and high expression of PTGES, PTGS2 was found on D14, D15 and D21; PTGFR was found on D8 and D12–21. LNPEP may have a role in prostaglandin regulation and conceptus fixation by decreasing the availability of oxytocin. Further investigation on the role embryonic LNPEP during pregnancy is warranted. |
| first_indexed | 2024-03-09T12:13:57Z |
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| institution | Directory Open Access Journal |
| issn | 2076-2615 |
| language | English |
| last_indexed | 2024-03-09T12:13:57Z |
| publishDate | 2022-03-01 |
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| record_format | Article |
| series | Animals |
| spelling | doaj.art-83bc4ac54d82475f99ce271b162ec11b2023-11-30T22:49:21ZengMDPI AGAnimals2076-26152022-03-0112779910.3390/ani12070799Expression of Oxytocin/Neurophysin I and Oxytocinase in the Equine Conceptus from Day 8 to Day 21 Post-OvulationMariana Diel de Amorim0Claudia Klein1Robert Foster2Lynn Dong3Maria Fernanda Lopez-Rodriguez4Claire Card5Department of Clinical Sciences, Cornell University, 930 Campus Rd, Ithaca, NY 14853, USADepartment of Veterinary Clinical and Diagnostic Science, University of Calgary, 2500 University Drive NW, Calgary, AB T2N 1N4, CanadaDepartment of Pathobiology, Ontario Veterinary College, University of Guelph, 50 Stone Road E, Guelph, ON N1G 2W1, CanadaImmunopathology Research and Development Laboratory, Department of Biomedical Sciences, Cornell University, 930 Campus Rd, Ithaca, NY 14853, USADepartment of Large Animal Clinical Sciences, Western College of Veterinary Medicine, 52 Campus Drive, Saskatoon, SK S7N 5B4, CanadaDepartment of Large Animal Clinical Sciences, Western College of Veterinary Medicine, 52 Campus Drive, Saskatoon, SK S7N 5B4, CanadaLeucyl and cystinyl aminopeptidase (LNPEP/oxytocinase) is an enzyme that metabolizes oxytocin in serum and tissues. The presence of oxytocin/neurophysin I (OXT), oxytocin and LNPEP and their relationship to other genes is unknown in the equine conceptus. Our objective was to characterize gene expression of LNPEP and OXT on D8, 10, 12, 14, 15, 16 and 21 conceptuses in relationship to other genes. Immunohistochemistry, western blot and liquid chromatography with tandem mass spectrometry (LC-MS/MS) were used for identification of oxytocin and LNPEP in D15, 16 and 18 conceptuses. LNPEP was increased at D15 compared to D10, was immunolocalized in the equine trophectoderm and endoderm, and protein was confirmed by LC-MS/MS. Maximal abundance of OXT was at D21, and lowest on D12 and D14, but no protein was identified. OXTR abundance was highest on D14 and D21. LNPEP was correlated with PTGFR and PTGES on D12 and D14–D15, and high expression of PTGES, PTGS2 was found on D14, D15 and D21; PTGFR was found on D8 and D12–21. LNPEP may have a role in prostaglandin regulation and conceptus fixation by decreasing the availability of oxytocin. Further investigation on the role embryonic LNPEP during pregnancy is warranted.https://www.mdpi.com/2076-2615/12/7/799equine conceptusglucose transportersleucyl and cystinyl aminopeptidaseoxytocin/neurophysin Iprostaglandin |
| spellingShingle | Mariana Diel de Amorim Claudia Klein Robert Foster Lynn Dong Maria Fernanda Lopez-Rodriguez Claire Card Expression of Oxytocin/Neurophysin I and Oxytocinase in the Equine Conceptus from Day 8 to Day 21 Post-Ovulation Animals equine conceptus glucose transporters leucyl and cystinyl aminopeptidase oxytocin/neurophysin I prostaglandin |
| title | Expression of Oxytocin/Neurophysin I and Oxytocinase in the Equine Conceptus from Day 8 to Day 21 Post-Ovulation |
| title_full | Expression of Oxytocin/Neurophysin I and Oxytocinase in the Equine Conceptus from Day 8 to Day 21 Post-Ovulation |
| title_fullStr | Expression of Oxytocin/Neurophysin I and Oxytocinase in the Equine Conceptus from Day 8 to Day 21 Post-Ovulation |
| title_full_unstemmed | Expression of Oxytocin/Neurophysin I and Oxytocinase in the Equine Conceptus from Day 8 to Day 21 Post-Ovulation |
| title_short | Expression of Oxytocin/Neurophysin I and Oxytocinase in the Equine Conceptus from Day 8 to Day 21 Post-Ovulation |
| title_sort | expression of oxytocin neurophysin i and oxytocinase in the equine conceptus from day 8 to day 21 post ovulation |
| topic | equine conceptus glucose transporters leucyl and cystinyl aminopeptidase oxytocin/neurophysin I prostaglandin |
| url | https://www.mdpi.com/2076-2615/12/7/799 |
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