Normative mice retinal thickness: 16-month longitudinal characterization of wild-type mice and changes in a model of Alzheimer's disease
Animal models of disease are paramount to understand retinal development, the pathophysiology of eye diseases, and to study neurodegeneration using optical coherence tomography (OCT) data. In this study, we present a comprehensive normative database of retinal thickness in C57BL6/129S mice using spe...
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| Format: | Article |
| Language: | English |
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Frontiers Media S.A.
2023-04-01
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| Series: | Frontiers in Aging Neuroscience |
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| Online Access: | https://www.frontiersin.org/articles/10.3389/fnagi.2023.1161847/full |
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| author | Ana Batista Pedro Guimarães João Martins João Martins João Martins João Martins Paula I. Moreira Paula I. Moreira Paula I. Moreira Paula I. Moreira António Francisco Ambrósio António Francisco Ambrósio António Francisco Ambrósio Miguel Castelo-Branco Miguel Castelo-Branco Pedro Serranho Pedro Serranho Rui Bernardes Rui Bernardes |
| author_facet | Ana Batista Pedro Guimarães João Martins João Martins João Martins João Martins Paula I. Moreira Paula I. Moreira Paula I. Moreira Paula I. Moreira António Francisco Ambrósio António Francisco Ambrósio António Francisco Ambrósio Miguel Castelo-Branco Miguel Castelo-Branco Pedro Serranho Pedro Serranho Rui Bernardes Rui Bernardes |
| author_sort | Ana Batista |
| collection | DOAJ |
| description | Animal models of disease are paramount to understand retinal development, the pathophysiology of eye diseases, and to study neurodegeneration using optical coherence tomography (OCT) data. In this study, we present a comprehensive normative database of retinal thickness in C57BL6/129S mice using spectral-domain OCT data. The database covers a longitudinal period of 16 months, from 1 to 16 months of age, and provides valuable insights into retinal development and changes over time. Our findings reveal that total retinal thickness decreases with age, while the thickness of individual retinal layers and layer aggregates changes in different ways. For example, the outer plexiform layer (OPL), photoreceptor inner segments (ILS), and retinal pigment epithelium (RPE) thickened over time, whereas other retinal layers and layer aggregates became thinner. Additionally, we compare the retinal thickness of wild-type (WT) mice with an animal model of Alzheimer's disease (3 × Tg-AD) and show that the transgenic mice exhibit a decrease in total retinal thickness compared to age-matched WT mice, with statistically significant differences observed at all evaluated ages. This normative database of retinal thickness in mice will serve as a reference for future studies on retinal changes in neurodegenerative and eye diseases and will further our understanding of the pathophysiology of these conditions. |
| first_indexed | 2024-04-09T19:16:55Z |
| format | Article |
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| institution | Directory Open Access Journal |
| issn | 1663-4365 |
| language | English |
| last_indexed | 2025-03-22T01:23:59Z |
| publishDate | 2023-04-01 |
| publisher | Frontiers Media S.A. |
| record_format | Article |
| series | Frontiers in Aging Neuroscience |
| spelling | doaj.art-83bf9c761e2847f2a0f380954acccc262024-05-10T11:15:42ZengFrontiers Media S.A.Frontiers in Aging Neuroscience1663-43652023-04-011510.3389/fnagi.2023.11618471161847Normative mice retinal thickness: 16-month longitudinal characterization of wild-type mice and changes in a model of Alzheimer's diseaseAna Batista0Pedro Guimarães1João Martins2João Martins3João Martins4João Martins5Paula I. Moreira6Paula I. Moreira7Paula I. Moreira8Paula I. Moreira9António Francisco Ambrósio10António Francisco Ambrósio11António Francisco Ambrósio12Miguel Castelo-Branco13Miguel Castelo-Branco14Pedro Serranho15Pedro Serranho16Rui Bernardes17Rui Bernardes18Coimbra Institute for Biomedical Imaging and Translational Research (CIBIT), Institute for Nuclear Sciences Applied to Health (ICNAS), University of Coimbra, Coimbra, PortugalCoimbra Institute for Biomedical Imaging and Translational Research (CIBIT), Institute for Nuclear Sciences Applied to Health (ICNAS), University of Coimbra, Coimbra, PortugalCoimbra Institute for Biomedical Imaging and Translational Research (CIBIT), Institute for Nuclear Sciences Applied to Health (ICNAS), University of Coimbra, Coimbra, PortugalCoimbra Institute for Clinical and Biomedical Research (iCBR), Faculty of Medicine (FMUC), University of Coimbra, Coimbra, PortugalCenter for Innovative Biomedicine and Biotechnology (CIBB), University of Coimbra, Coimbra, PortugalClinical Academic Center of Coimbra (CACC), Faculty of Medicine (FMUC), University of Coimbra, Coimbra, PortugalCenter for Innovative Biomedicine and Biotechnology (CIBB), University of Coimbra, Coimbra, PortugalClinical Academic Center of Coimbra (CACC), Faculty of Medicine (FMUC), University of Coimbra, Coimbra, PortugalLaboratory of Physiology, Faculty of Medicine (FMUC), University of Coimbra, Coimbra, PortugalCenter for Neuroscience and Cell Biology (CNC), University of Coimbra, Coimbra, PortugalCoimbra Institute for Clinical and Biomedical Research (iCBR), Faculty of Medicine (FMUC), University of Coimbra, Coimbra, PortugalCenter for Innovative Biomedicine and Biotechnology (CIBB), University of Coimbra, Coimbra, PortugalClinical Academic Center of Coimbra (CACC), Faculty of Medicine (FMUC), University of Coimbra, Coimbra, PortugalCoimbra Institute for Biomedical Imaging and Translational Research (CIBIT), Institute for Nuclear Sciences Applied to Health (ICNAS), University of Coimbra, Coimbra, PortugalClinical Academic Center of Coimbra (CACC), Faculty of Medicine (FMUC), University of Coimbra, Coimbra, PortugalCoimbra Institute for Biomedical Imaging and Translational Research (CIBIT), Institute for Nuclear Sciences Applied to Health (ICNAS), University of Coimbra, Coimbra, PortugalMathematics Section, Department of Sciences and Technology, Universidade Aberta, Lisbon, PortugalCoimbra Institute for Biomedical Imaging and Translational Research (CIBIT), Institute for Nuclear Sciences Applied to Health (ICNAS), University of Coimbra, Coimbra, PortugalClinical Academic Center of Coimbra (CACC), Faculty of Medicine (FMUC), University of Coimbra, Coimbra, PortugalAnimal models of disease are paramount to understand retinal development, the pathophysiology of eye diseases, and to study neurodegeneration using optical coherence tomography (OCT) data. In this study, we present a comprehensive normative database of retinal thickness in C57BL6/129S mice using spectral-domain OCT data. The database covers a longitudinal period of 16 months, from 1 to 16 months of age, and provides valuable insights into retinal development and changes over time. Our findings reveal that total retinal thickness decreases with age, while the thickness of individual retinal layers and layer aggregates changes in different ways. For example, the outer plexiform layer (OPL), photoreceptor inner segments (ILS), and retinal pigment epithelium (RPE) thickened over time, whereas other retinal layers and layer aggregates became thinner. Additionally, we compare the retinal thickness of wild-type (WT) mice with an animal model of Alzheimer's disease (3 × Tg-AD) and show that the transgenic mice exhibit a decrease in total retinal thickness compared to age-matched WT mice, with statistically significant differences observed at all evaluated ages. This normative database of retinal thickness in mice will serve as a reference for future studies on retinal changes in neurodegenerative and eye diseases and will further our understanding of the pathophysiology of these conditions.https://www.frontiersin.org/articles/10.3389/fnagi.2023.1161847/fulloptical coherence tomographyretinal thicknessnormative dataAlzheimer's disease3 × Tg-AD animal model |
| spellingShingle | Ana Batista Pedro Guimarães João Martins João Martins João Martins João Martins Paula I. Moreira Paula I. Moreira Paula I. Moreira Paula I. Moreira António Francisco Ambrósio António Francisco Ambrósio António Francisco Ambrósio Miguel Castelo-Branco Miguel Castelo-Branco Pedro Serranho Pedro Serranho Rui Bernardes Rui Bernardes Normative mice retinal thickness: 16-month longitudinal characterization of wild-type mice and changes in a model of Alzheimer's disease Frontiers in Aging Neuroscience optical coherence tomography retinal thickness normative data Alzheimer's disease 3 × Tg-AD animal model |
| title | Normative mice retinal thickness: 16-month longitudinal characterization of wild-type mice and changes in a model of Alzheimer's disease |
| title_full | Normative mice retinal thickness: 16-month longitudinal characterization of wild-type mice and changes in a model of Alzheimer's disease |
| title_fullStr | Normative mice retinal thickness: 16-month longitudinal characterization of wild-type mice and changes in a model of Alzheimer's disease |
| title_full_unstemmed | Normative mice retinal thickness: 16-month longitudinal characterization of wild-type mice and changes in a model of Alzheimer's disease |
| title_short | Normative mice retinal thickness: 16-month longitudinal characterization of wild-type mice and changes in a model of Alzheimer's disease |
| title_sort | normative mice retinal thickness 16 month longitudinal characterization of wild type mice and changes in a model of alzheimer s disease |
| topic | optical coherence tomography retinal thickness normative data Alzheimer's disease 3 × Tg-AD animal model |
| url | https://www.frontiersin.org/articles/10.3389/fnagi.2023.1161847/full |
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