RNA sensor response in HeLa cells for transfected mRNAs prepared in vitro by SP6 and HiT7 RNA polymerases: A comparative study
In vitro transcribed (IVT) synthetic mRNAs are in high demand due to their attractive bench to clinic translational processes. Mainly, the procedure to make IVT mRNA using bacteriophage RNA polymerases (RNAP) is relatively uncomplicated and scalable to produce large quantities in a short time period...
Main Authors: | Siranjeevi Nagaraj, Anna Stankiewicz-Drogon, Edward Darzynkiewicz, Renata Grzela |
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Format: | Article |
Language: | English |
Published: |
Frontiers Media S.A.
2022-11-01
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Series: | Frontiers in Bioengineering and Biotechnology |
Subjects: | |
Online Access: | https://www.frontiersin.org/articles/10.3389/fbioe.2022.1017934/full |
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