Nanoclay-Doped Electrospun Nanofibers for Tissue Engineering: Investigation on the Structural Modifications in Physiological Environment
Dalila Miele,1 Marco Ruggeri,1 Barbara Vigani,1 Cesar Viseras,2 Francesca Natali,3 Elena Del Favero,4 Silvia Rossi,1 Giuseppina Sandri1 1Department of Drug Sciences, University of Pavia, Pavia, Italy; 2Department of Pharmacy and Pharmaceutical Technology, School of Pharmacy, University of Granada, G...
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| Format: | Article |
| Language: | English |
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Dove Medical Press
2023-12-01
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| Series: | International Journal of Nanomedicine |
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| Online Access: | https://www.dovepress.com/nanoclay-doped-electrospun-nanofibers-for-tissue-engineering-investiga-peer-reviewed-fulltext-article-IJN |
| _version_ | 1797391156293664768 |
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| author | Miele D Ruggeri M Vigani B Viseras C Natali F Del Favero E Rossi S Sandri G |
| author_facet | Miele D Ruggeri M Vigani B Viseras C Natali F Del Favero E Rossi S Sandri G |
| author_sort | Miele D |
| collection | DOAJ |
| description | Dalila Miele,1 Marco Ruggeri,1 Barbara Vigani,1 Cesar Viseras,2 Francesca Natali,3 Elena Del Favero,4 Silvia Rossi,1 Giuseppina Sandri1 1Department of Drug Sciences, University of Pavia, Pavia, Italy; 2Department of Pharmacy and Pharmaceutical Technology, School of Pharmacy, University of Granada, Granada, Spain; 3Institut Laue-Langevin, Grenoble, France; 4Department of Medical Biotechnology and Translational Medicine, University of Milano, Segrate Milano, ItalyCorrespondence: Giuseppina Sandri, Tel +390382987728, Email giuseppina.sandri@unipv.itBackground: Clay minerals are nanomaterials that have recently been recognized as enabling excipients that can promote cell adhesion, proliferation, and differentiation. When nanoclays are loaded in a 3D polymeric nanostructure, the cell–substrate interaction is enhanced, and other bioactive properties are optimized.Purpose: In this study, hectorite (HEC)- and montmorillonite (MMT)-doped polymeric scaffolds were explored for the treatment of deep and chronic skin lesions.Methods: Scaffolds were manufactured by means of electrospinning and then crosslinked by heating. Physicochemical analyses were correlated with in vitro biopharmaceutical characterization to predict the in vivo fate of the clay-doped scaffolds.Results and Discussion: The addition of MMT or HEC to the polymeric scaffold framework modifies the surface arrangement and, consequently, the potential of the scaffolds to interact with biological proteins. The presence of nanoclays alters the nanofiber morphology and size, and MMT doping increases wettability and protein adhesion. This has an impact on fibroblast behavior in a shorter time since scaffold stiffness facilitates cell adhesion and cell proliferation.Conclusion: MMT proved to perform better than HEC, and this could be related to its higher hydrophilicity and protein adhesion. Keywords: nanomaterials, montmorillonite, hectorite, chitosan, nanofibers, electrospinning, tissue engineering |
| first_indexed | 2024-03-08T23:28:58Z |
| format | Article |
| id | doaj.art-83c0a97b58b44e1b982fe29e02459505 |
| institution | Directory Open Access Journal |
| issn | 1178-2013 |
| language | English |
| last_indexed | 2024-03-08T23:28:58Z |
| publishDate | 2023-12-01 |
| publisher | Dove Medical Press |
| record_format | Article |
| series | International Journal of Nanomedicine |
| spelling | doaj.art-83c0a97b58b44e1b982fe29e024595052023-12-14T17:01:45ZengDove Medical PressInternational Journal of Nanomedicine1178-20132023-12-01Volume 187695771089027Nanoclay-Doped Electrospun Nanofibers for Tissue Engineering: Investigation on the Structural Modifications in Physiological EnvironmentMiele DRuggeri MVigani BViseras CNatali FDel Favero ERossi SSandri GDalila Miele,1 Marco Ruggeri,1 Barbara Vigani,1 Cesar Viseras,2 Francesca Natali,3 Elena Del Favero,4 Silvia Rossi,1 Giuseppina Sandri1 1Department of Drug Sciences, University of Pavia, Pavia, Italy; 2Department of Pharmacy and Pharmaceutical Technology, School of Pharmacy, University of Granada, Granada, Spain; 3Institut Laue-Langevin, Grenoble, France; 4Department of Medical Biotechnology and Translational Medicine, University of Milano, Segrate Milano, ItalyCorrespondence: Giuseppina Sandri, Tel +390382987728, Email giuseppina.sandri@unipv.itBackground: Clay minerals are nanomaterials that have recently been recognized as enabling excipients that can promote cell adhesion, proliferation, and differentiation. When nanoclays are loaded in a 3D polymeric nanostructure, the cell–substrate interaction is enhanced, and other bioactive properties are optimized.Purpose: In this study, hectorite (HEC)- and montmorillonite (MMT)-doped polymeric scaffolds were explored for the treatment of deep and chronic skin lesions.Methods: Scaffolds were manufactured by means of electrospinning and then crosslinked by heating. Physicochemical analyses were correlated with in vitro biopharmaceutical characterization to predict the in vivo fate of the clay-doped scaffolds.Results and Discussion: The addition of MMT or HEC to the polymeric scaffold framework modifies the surface arrangement and, consequently, the potential of the scaffolds to interact with biological proteins. The presence of nanoclays alters the nanofiber morphology and size, and MMT doping increases wettability and protein adhesion. This has an impact on fibroblast behavior in a shorter time since scaffold stiffness facilitates cell adhesion and cell proliferation.Conclusion: MMT proved to perform better than HEC, and this could be related to its higher hydrophilicity and protein adhesion. Keywords: nanomaterials, montmorillonite, hectorite, chitosan, nanofibers, electrospinning, tissue engineeringhttps://www.dovepress.com/nanoclay-doped-electrospun-nanofibers-for-tissue-engineering-investiga-peer-reviewed-fulltext-article-IJNnanomaterialsmontmorillonitehectoritechitosannanofiberselectrospinningtissue engineering |
| spellingShingle | Miele D Ruggeri M Vigani B Viseras C Natali F Del Favero E Rossi S Sandri G Nanoclay-Doped Electrospun Nanofibers for Tissue Engineering: Investigation on the Structural Modifications in Physiological Environment International Journal of Nanomedicine nanomaterials montmorillonite hectorite chitosan nanofibers electrospinning tissue engineering |
| title | Nanoclay-Doped Electrospun Nanofibers for Tissue Engineering: Investigation on the Structural Modifications in Physiological Environment |
| title_full | Nanoclay-Doped Electrospun Nanofibers for Tissue Engineering: Investigation on the Structural Modifications in Physiological Environment |
| title_fullStr | Nanoclay-Doped Electrospun Nanofibers for Tissue Engineering: Investigation on the Structural Modifications in Physiological Environment |
| title_full_unstemmed | Nanoclay-Doped Electrospun Nanofibers for Tissue Engineering: Investigation on the Structural Modifications in Physiological Environment |
| title_short | Nanoclay-Doped Electrospun Nanofibers for Tissue Engineering: Investigation on the Structural Modifications in Physiological Environment |
| title_sort | nanoclay doped electrospun nanofibers for tissue engineering investigation on the structural modifications in physiological environment |
| topic | nanomaterials montmorillonite hectorite chitosan nanofibers electrospinning tissue engineering |
| url | https://www.dovepress.com/nanoclay-doped-electrospun-nanofibers-for-tissue-engineering-investiga-peer-reviewed-fulltext-article-IJN |
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