Protective Effect of Ganoderma lucidum Spore Powder on Acute Liver Injury in Mice and its Regulation of Gut Microbiota
Background: Ganoderma lucidum spore powder (GLSP) has abundant pharmacological activities. However, the difference in the hepatoprotective function of sporoderm-broken and sporoderm-unbroken Ganoderma spore powder has not been studied. This study is the first to investigate the effects of both sporo...
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| Format: | Article |
| Language: | English |
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IMR Press
2023-02-01
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| Series: | Frontiers in Bioscience-Landmark |
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| Online Access: | https://www.imrpress.com/journal/FBL/28/2/10.31083/j.fbl2802023 |
| _version_ | 1811160694564847616 |
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| author | Yue Leng Fang Wang Changbao Chen Xilin Wan Xinyang Li Huan Wang Shumin Wang |
| author_facet | Yue Leng Fang Wang Changbao Chen Xilin Wan Xinyang Li Huan Wang Shumin Wang |
| author_sort | Yue Leng |
| collection | DOAJ |
| description | Background: Ganoderma lucidum spore powder (GLSP) has abundant pharmacological activities. However, the difference in the hepatoprotective function of sporoderm-broken and sporoderm-unbroken Ganoderma spore powder has not been studied. This study is the first to investigate the effects of both sporoderm-damaged and sporoderm-intact GLSP on the improvement of acute alcoholic liver injury in mice and gut microbiota of mice. Methods: Serum aspartate aminotransferase (AST) and alanine aminotransferase (ALT) levels and interleukin 1β (IL-1β), interleukin 18 (IL-18), and tumor necrosis factor-α (TNF-α) levels in liver tissues from mice in each group were detected by enzyme-linked immunosorbent assay (ELISA) kits, and histological analysis of liver tissue sections was performed to evaluate the liver-protecting effects of both sporoderm-broken and sporoderm-unbroken GLSP. Additionally, 16S rDNA sequencing of feces from the bowels of mice was performed to compare the regulatory effects of both sporoderm-broken and sporoderm-unbroken GLSP on the gut microbiota of mice. Results: Compared with those in the 50% ethanol model group (MG), sporoderm-broken GLSP significantly reduced serum AST and ALT levels (p < 0.0001) and the release of the inflammatory factors, including IL-1β, IL-18, and TNF-α (p < 0.0001), and effectively improved the pathological state of liver cells; sporoderm-unbroken GLSP significantly reduced the ALT content (p = 0.0002) and the release of the inflammatory factors, including IL-1β (p < 0.0001), IL-18 (p = 0.0018), and TNF-α (p = 0.0005), and reduced the serum AST content, but the reduction was not significant; compared with the gut microbiota of the MG, sporoderm-broken GLSP reduced the levels of Verrucomicrobia and Escherichia_Shigella, increased the relative abundance of beneficial bacteria such as Bacteroidetes, and decreased the abundance levels of harmful bacteria, such as Proteobacteria and Candidatus_Saccharibacteria; sporoderm-unbroken GLSP could reduce the abundance levels of harmful bacteria, such as Verrucomicrobia and Candidatus_Saccharibacteria; and GLSP treatment alleviates the downregulation of the levels of translation, ribosome structure and biogenesis, and lipid transport and metabolism in liver-injured mice; Conclusions: GLSP can alleviate the imbalance of gut microbiota and improve liver injury, and the effect of sporoderm-broken GLSP is better. |
| first_indexed | 2024-04-10T06:01:44Z |
| format | Article |
| id | doaj.art-83c4cba2edeb4938add276e2ab77150a |
| institution | Directory Open Access Journal |
| issn | 2768-6701 |
| language | English |
| last_indexed | 2024-04-10T06:01:44Z |
| publishDate | 2023-02-01 |
| publisher | IMR Press |
| record_format | Article |
| series | Frontiers in Bioscience-Landmark |
| spelling | doaj.art-83c4cba2edeb4938add276e2ab77150a2023-03-03T08:24:30ZengIMR PressFrontiers in Bioscience-Landmark2768-67012023-02-012822310.31083/j.fbl2802023S2768-6701(22)00724-9Protective Effect of Ganoderma lucidum Spore Powder on Acute Liver Injury in Mice and its Regulation of Gut MicrobiotaYue Leng0Fang Wang1Changbao Chen2Xilin Wan3Xinyang Li4Huan Wang5Shumin Wang6Department of Ginseng Scientific Research Institute, Changchun University of Chinese Medicine, 130117 Changchun, Jilin, ChinaDepartment of Ginseng Scientific Research Institute, Changchun University of Chinese Medicine, 130117 Changchun, Jilin, ChinaDepartment of Ginseng Scientific Research Institute, Changchun University of Chinese Medicine, 130117 Changchun, Jilin, ChinaDepartment of Ginseng Scientific Research Institute, Changchun University of Chinese Medicine, 130117 Changchun, Jilin, ChinaDepartment of Ginseng Scientific Research Institute, Changchun University of Chinese Medicine, 130117 Changchun, Jilin, ChinaDepartment of Ginseng Scientific Research Institute, Changchun University of Chinese Medicine, 130117 Changchun, Jilin, ChinaDepartment of Ginseng Scientific Research Institute, Changchun University of Chinese Medicine, 130117 Changchun, Jilin, ChinaBackground: Ganoderma lucidum spore powder (GLSP) has abundant pharmacological activities. However, the difference in the hepatoprotective function of sporoderm-broken and sporoderm-unbroken Ganoderma spore powder has not been studied. This study is the first to investigate the effects of both sporoderm-damaged and sporoderm-intact GLSP on the improvement of acute alcoholic liver injury in mice and gut microbiota of mice. Methods: Serum aspartate aminotransferase (AST) and alanine aminotransferase (ALT) levels and interleukin 1β (IL-1β), interleukin 18 (IL-18), and tumor necrosis factor-α (TNF-α) levels in liver tissues from mice in each group were detected by enzyme-linked immunosorbent assay (ELISA) kits, and histological analysis of liver tissue sections was performed to evaluate the liver-protecting effects of both sporoderm-broken and sporoderm-unbroken GLSP. Additionally, 16S rDNA sequencing of feces from the bowels of mice was performed to compare the regulatory effects of both sporoderm-broken and sporoderm-unbroken GLSP on the gut microbiota of mice. Results: Compared with those in the 50% ethanol model group (MG), sporoderm-broken GLSP significantly reduced serum AST and ALT levels (p < 0.0001) and the release of the inflammatory factors, including IL-1β, IL-18, and TNF-α (p < 0.0001), and effectively improved the pathological state of liver cells; sporoderm-unbroken GLSP significantly reduced the ALT content (p = 0.0002) and the release of the inflammatory factors, including IL-1β (p < 0.0001), IL-18 (p = 0.0018), and TNF-α (p = 0.0005), and reduced the serum AST content, but the reduction was not significant; compared with the gut microbiota of the MG, sporoderm-broken GLSP reduced the levels of Verrucomicrobia and Escherichia_Shigella, increased the relative abundance of beneficial bacteria such as Bacteroidetes, and decreased the abundance levels of harmful bacteria, such as Proteobacteria and Candidatus_Saccharibacteria; sporoderm-unbroken GLSP could reduce the abundance levels of harmful bacteria, such as Verrucomicrobia and Candidatus_Saccharibacteria; and GLSP treatment alleviates the downregulation of the levels of translation, ribosome structure and biogenesis, and lipid transport and metabolism in liver-injured mice; Conclusions: GLSP can alleviate the imbalance of gut microbiota and improve liver injury, and the effect of sporoderm-broken GLSP is better.https://www.imrpress.com/journal/FBL/28/2/10.31083/j.fbl2802023sporoderm-broken ganoderma lucidum spore powdergut microbiotaalcoholic liver diseasediammonium glycyrrhizinateliver protection |
| spellingShingle | Yue Leng Fang Wang Changbao Chen Xilin Wan Xinyang Li Huan Wang Shumin Wang Protective Effect of Ganoderma lucidum Spore Powder on Acute Liver Injury in Mice and its Regulation of Gut Microbiota Frontiers in Bioscience-Landmark sporoderm-broken ganoderma lucidum spore powder gut microbiota alcoholic liver disease diammonium glycyrrhizinate liver protection |
| title | Protective Effect of Ganoderma lucidum Spore Powder on Acute Liver Injury in Mice and its Regulation of Gut Microbiota |
| title_full | Protective Effect of Ganoderma lucidum Spore Powder on Acute Liver Injury in Mice and its Regulation of Gut Microbiota |
| title_fullStr | Protective Effect of Ganoderma lucidum Spore Powder on Acute Liver Injury in Mice and its Regulation of Gut Microbiota |
| title_full_unstemmed | Protective Effect of Ganoderma lucidum Spore Powder on Acute Liver Injury in Mice and its Regulation of Gut Microbiota |
| title_short | Protective Effect of Ganoderma lucidum Spore Powder on Acute Liver Injury in Mice and its Regulation of Gut Microbiota |
| title_sort | protective effect of ganoderma lucidum spore powder on acute liver injury in mice and its regulation of gut microbiota |
| topic | sporoderm-broken ganoderma lucidum spore powder gut microbiota alcoholic liver disease diammonium glycyrrhizinate liver protection |
| url | https://www.imrpress.com/journal/FBL/28/2/10.31083/j.fbl2802023 |
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