Comparative Characterization of Vibrio cholerae O1 from Five Sub-Saharan African Countries Using Various Phenotypic and Genotypic Techniques.
We used standardized methodologies to characterize Vibrio cholerae O1 isolates from Guinea, Democratic Republic of the Congo (DRC), Togo, Côte d'Ivoire and Mozambique. We investigated 257 human isolates collected in 2010 to 2013. DRC isolates serotyped O1 Inaba, while isolates from other countr...
| Main Authors: | , , , , , , , , , , , , , |
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| Format: | Article |
| Language: | English |
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Public Library of Science (PLoS)
2015-01-01
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| Series: | PLoS ONE |
| Online Access: | http://europepmc.org/articles/PMC4659613?pdf=render |
| _version_ | 1818949546855628800 |
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| author | Anthony M Smith Berthe-Marie Njanpop-Lafourcade Martin A Mengel Bradford D Gessner Delphine Sauvageot Bawimodom Bidjada Berthe N Miwanda Diallo M Saliou Adèle Kacou N'Douba José P Langa Husna Ismail Nomsa Tau Arvinda Sooka Karen H Keddy |
| author_facet | Anthony M Smith Berthe-Marie Njanpop-Lafourcade Martin A Mengel Bradford D Gessner Delphine Sauvageot Bawimodom Bidjada Berthe N Miwanda Diallo M Saliou Adèle Kacou N'Douba José P Langa Husna Ismail Nomsa Tau Arvinda Sooka Karen H Keddy |
| author_sort | Anthony M Smith |
| collection | DOAJ |
| description | We used standardized methodologies to characterize Vibrio cholerae O1 isolates from Guinea, Democratic Republic of the Congo (DRC), Togo, Côte d'Ivoire and Mozambique. We investigated 257 human isolates collected in 2010 to 2013. DRC isolates serotyped O1 Inaba, while isolates from other countries serotyped O1 Ogawa. All isolates were biotype El Tor and positive for cholera toxin. All isolates showed multidrug resistance but lacked ciprofloxacin resistance. Antimicrobial susceptibility profiles of isolates varied between countries. In particular, the susceptibility profile of isolates from Mozambique (East-Africa) included resistance to ceftriaxone and was distinctly different to the susceptibility profiles of isolates from countries located in West- and Central-Africa. Molecular subtyping of isolates using pulsed-field gel electrophoresis (PFGE) analysis showed a complex relationship among isolates. Some PFGE patterns were unique to particular countries and clustered by country; while other PFGE patterns were shared by isolates from multiple countries, indicating that the same genetic lineage is present in multiple countries. Our data add to a better understanding of cholera epidemiology in Africa. |
| first_indexed | 2024-12-20T09:04:25Z |
| format | Article |
| id | doaj.art-83c6432d0566481c92ccd441b738991f |
| institution | Directory Open Access Journal |
| issn | 1932-6203 |
| language | English |
| last_indexed | 2024-12-20T09:04:25Z |
| publishDate | 2015-01-01 |
| publisher | Public Library of Science (PLoS) |
| record_format | Article |
| series | PLoS ONE |
| spelling | doaj.art-83c6432d0566481c92ccd441b738991f2022-12-21T19:45:45ZengPublic Library of Science (PLoS)PLoS ONE1932-62032015-01-011011e014298910.1371/journal.pone.0142989Comparative Characterization of Vibrio cholerae O1 from Five Sub-Saharan African Countries Using Various Phenotypic and Genotypic Techniques.Anthony M SmithBerthe-Marie Njanpop-LafourcadeMartin A MengelBradford D GessnerDelphine SauvageotBawimodom BidjadaBerthe N MiwandaDiallo M SaliouAdèle Kacou N'DoubaJosé P LangaHusna IsmailNomsa TauArvinda SookaKaren H KeddyWe used standardized methodologies to characterize Vibrio cholerae O1 isolates from Guinea, Democratic Republic of the Congo (DRC), Togo, Côte d'Ivoire and Mozambique. We investigated 257 human isolates collected in 2010 to 2013. DRC isolates serotyped O1 Inaba, while isolates from other countries serotyped O1 Ogawa. All isolates were biotype El Tor and positive for cholera toxin. All isolates showed multidrug resistance but lacked ciprofloxacin resistance. Antimicrobial susceptibility profiles of isolates varied between countries. In particular, the susceptibility profile of isolates from Mozambique (East-Africa) included resistance to ceftriaxone and was distinctly different to the susceptibility profiles of isolates from countries located in West- and Central-Africa. Molecular subtyping of isolates using pulsed-field gel electrophoresis (PFGE) analysis showed a complex relationship among isolates. Some PFGE patterns were unique to particular countries and clustered by country; while other PFGE patterns were shared by isolates from multiple countries, indicating that the same genetic lineage is present in multiple countries. Our data add to a better understanding of cholera epidemiology in Africa.http://europepmc.org/articles/PMC4659613?pdf=render |
| spellingShingle | Anthony M Smith Berthe-Marie Njanpop-Lafourcade Martin A Mengel Bradford D Gessner Delphine Sauvageot Bawimodom Bidjada Berthe N Miwanda Diallo M Saliou Adèle Kacou N'Douba José P Langa Husna Ismail Nomsa Tau Arvinda Sooka Karen H Keddy Comparative Characterization of Vibrio cholerae O1 from Five Sub-Saharan African Countries Using Various Phenotypic and Genotypic Techniques. PLoS ONE |
| title | Comparative Characterization of Vibrio cholerae O1 from Five Sub-Saharan African Countries Using Various Phenotypic and Genotypic Techniques. |
| title_full | Comparative Characterization of Vibrio cholerae O1 from Five Sub-Saharan African Countries Using Various Phenotypic and Genotypic Techniques. |
| title_fullStr | Comparative Characterization of Vibrio cholerae O1 from Five Sub-Saharan African Countries Using Various Phenotypic and Genotypic Techniques. |
| title_full_unstemmed | Comparative Characterization of Vibrio cholerae O1 from Five Sub-Saharan African Countries Using Various Phenotypic and Genotypic Techniques. |
| title_short | Comparative Characterization of Vibrio cholerae O1 from Five Sub-Saharan African Countries Using Various Phenotypic and Genotypic Techniques. |
| title_sort | comparative characterization of vibrio cholerae o1 from five sub saharan african countries using various phenotypic and genotypic techniques |
| url | http://europepmc.org/articles/PMC4659613?pdf=render |
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