Antibiotic susceptibility and molecular characterization of resistance genes among Escherichia coli and among Salmonella subsp. in chicken food chains
Objective: To investigate the occurrence of resistance genes among Escherichia coli (E. coli) and Salmonella subsp. isolated in chicken food chains in Phnom Penh, 2012–2013. Methods: Six hundred eighty two E. coli and 181 Salmonella Albany, Corvallis, and Kentucky strains were examined for susceptib...
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| Format: | Article |
| Language: | English |
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Wolters Kluwer Medknow Publications
2017-07-01
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| Series: | Asian Pacific Journal of Tropical Biomedicine |
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| Online Access: | http://www.sciencedirect.com/science/article/pii/S222116911730672X |
| _version_ | 1818310431230394368 |
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| author | Yith Vuthy Kruy Sun Lay Heng Seiha Alexandra Kerleguer Awa Aidara-Kane |
| author_facet | Yith Vuthy Kruy Sun Lay Heng Seiha Alexandra Kerleguer Awa Aidara-Kane |
| author_sort | Yith Vuthy |
| collection | DOAJ |
| description | Objective: To investigate the occurrence of resistance genes among Escherichia coli (E. coli) and Salmonella subsp. isolated in chicken food chains in Phnom Penh, 2012–2013.
Methods: Six hundred eighty two E. coli and 181 Salmonella Albany, Corvallis, and Kentucky strains were examined for susceptibilities to eight antimicrobials and following resistance genes were identified by PCR: blaTem, StrA, aadA, sul1, sul2, gyrA, Tet (A), and Tet (B).
Results: E. coli presented high resistances to tetracycline, amoxicillin, and sulfamethoxazole (63.1%–76.1%). Salmonella Albany and Salmonella Kentucky traduced high resistance percentages to amoxicillin, tetracycline, sulfamethoxazole, and nalidixic acid (84.6%–100%). Among amoxicillin-resistant isolates, blaTem genes were observed for 62% of E. coli isolates and 20% of 65 Salmonella Kentucky. The StrA gene was prevalent in 36% of 331 aminoglycoside-resistant E. coli and 90% of 40 aminoglycoside-resistant Salmonella Corvallis. The sul2 gene was predominant among sulfamethoxazole-resistant isolates, for 56% of 431 E. coli and 53% of 66 Salmonella Corvallis; the sul1 gene was observed in 54% of Salmonella Albany. The Tet (A) resistance gene was prevalent in E. coli (86%), Salmonella Corvallis (82%), Salmonella Kentucky (84%). High percentages of gyrA genes observed among nalidixic-acid resistant E. coli (91%), Salmonella Albany (92%), Salmonella Corvallis (75%) and Salmonella Kentucky (85%).
Conclusions: Important occurrences of resistance gene were observed among E. coli and Salmonella in chicken food chains in Cambodia. |
| first_indexed | 2024-12-13T07:45:57Z |
| format | Article |
| id | doaj.art-83c99b4dde0040b0bcb8217392bccec0 |
| institution | Directory Open Access Journal |
| issn | 2221-1691 |
| language | English |
| last_indexed | 2024-12-13T07:45:57Z |
| publishDate | 2017-07-01 |
| publisher | Wolters Kluwer Medknow Publications |
| record_format | Article |
| series | Asian Pacific Journal of Tropical Biomedicine |
| spelling | doaj.art-83c99b4dde0040b0bcb8217392bccec02022-12-21T23:54:50ZengWolters Kluwer Medknow PublicationsAsian Pacific Journal of Tropical Biomedicine2221-16912017-07-017767067410.1016/j.apjtb.2017.07.002Antibiotic susceptibility and molecular characterization of resistance genes among Escherichia coli and among Salmonella subsp. in chicken food chainsYith Vuthy0Kruy Sun Lay1Heng Seiha2Alexandra Kerleguer3Awa Aidara-Kane4Institut Pasteur, Phnom Penh, CambodiaInstitut Pasteur, Phnom Penh, CambodiaInstitut Pasteur, Phnom Penh, CambodiaInstitut Pasteur, Phnom Penh, CambodiaWorld Health Organization, Geneva, SwitzerlandObjective: To investigate the occurrence of resistance genes among Escherichia coli (E. coli) and Salmonella subsp. isolated in chicken food chains in Phnom Penh, 2012–2013. Methods: Six hundred eighty two E. coli and 181 Salmonella Albany, Corvallis, and Kentucky strains were examined for susceptibilities to eight antimicrobials and following resistance genes were identified by PCR: blaTem, StrA, aadA, sul1, sul2, gyrA, Tet (A), and Tet (B). Results: E. coli presented high resistances to tetracycline, amoxicillin, and sulfamethoxazole (63.1%–76.1%). Salmonella Albany and Salmonella Kentucky traduced high resistance percentages to amoxicillin, tetracycline, sulfamethoxazole, and nalidixic acid (84.6%–100%). Among amoxicillin-resistant isolates, blaTem genes were observed for 62% of E. coli isolates and 20% of 65 Salmonella Kentucky. The StrA gene was prevalent in 36% of 331 aminoglycoside-resistant E. coli and 90% of 40 aminoglycoside-resistant Salmonella Corvallis. The sul2 gene was predominant among sulfamethoxazole-resistant isolates, for 56% of 431 E. coli and 53% of 66 Salmonella Corvallis; the sul1 gene was observed in 54% of Salmonella Albany. The Tet (A) resistance gene was prevalent in E. coli (86%), Salmonella Corvallis (82%), Salmonella Kentucky (84%). High percentages of gyrA genes observed among nalidixic-acid resistant E. coli (91%), Salmonella Albany (92%), Salmonella Corvallis (75%) and Salmonella Kentucky (85%). Conclusions: Important occurrences of resistance gene were observed among E. coli and Salmonella in chicken food chains in Cambodia.http://www.sciencedirect.com/science/article/pii/S222116911730672XChicken food chainsEscherichia coliSalmonellaResistance geneSusceptibility |
| spellingShingle | Yith Vuthy Kruy Sun Lay Heng Seiha Alexandra Kerleguer Awa Aidara-Kane Antibiotic susceptibility and molecular characterization of resistance genes among Escherichia coli and among Salmonella subsp. in chicken food chains Asian Pacific Journal of Tropical Biomedicine Chicken food chains Escherichia coli Salmonella Resistance gene Susceptibility |
| title | Antibiotic susceptibility and molecular characterization of resistance genes among Escherichia coli and among Salmonella subsp. in chicken food chains |
| title_full | Antibiotic susceptibility and molecular characterization of resistance genes among Escherichia coli and among Salmonella subsp. in chicken food chains |
| title_fullStr | Antibiotic susceptibility and molecular characterization of resistance genes among Escherichia coli and among Salmonella subsp. in chicken food chains |
| title_full_unstemmed | Antibiotic susceptibility and molecular characterization of resistance genes among Escherichia coli and among Salmonella subsp. in chicken food chains |
| title_short | Antibiotic susceptibility and molecular characterization of resistance genes among Escherichia coli and among Salmonella subsp. in chicken food chains |
| title_sort | antibiotic susceptibility and molecular characterization of resistance genes among escherichia coli and among salmonella subsp in chicken food chains |
| topic | Chicken food chains Escherichia coli Salmonella Resistance gene Susceptibility |
| url | http://www.sciencedirect.com/science/article/pii/S222116911730672X |
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