Waterproof Galvanometer Scanner-Based Handheld Photoacoustic Microscopy Probe for Wide-Field Vasculature Imaging In Vivo
Photoacoustic imaging (PAI) is a hybrid non-invasive imaging technique used to merge high optical contrast and high acoustic resolution in deep tissue. PAI has been extensively developed by utilizing its advantages that include deep imaging depth, high resolution, and label-free imaging. As a repres...
| Main Authors: | , , , , , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
MDPI AG
2021-07-01
|
| Series: | Photonics |
| Subjects: | |
| Online Access: | https://www.mdpi.com/2304-6732/8/8/305 |
| _version_ | 1827684300446760960 |
|---|---|
| author | Daewoon Seong Sangyeob Han Jaeyul Lee Euimin Lee Yoonseok Kim Junsoo Lee Mansik Jeon Jeehyun Kim |
| author_facet | Daewoon Seong Sangyeob Han Jaeyul Lee Euimin Lee Yoonseok Kim Junsoo Lee Mansik Jeon Jeehyun Kim |
| author_sort | Daewoon Seong |
| collection | DOAJ |
| description | Photoacoustic imaging (PAI) is a hybrid non-invasive imaging technique used to merge high optical contrast and high acoustic resolution in deep tissue. PAI has been extensively developed by utilizing its advantages that include deep imaging depth, high resolution, and label-free imaging. As a representative implementation of PAI, photoacoustic microscopy (PAM) has been used in preclinical and clinical studies for its micron-scale spatial resolution capability with high optical absorption contrast. Several handheld and portable PAM systems have been developed that improve its applicability to several fields, making it versatile. In this study, we developed a laboratory-customized, two-axis, waterproof, galvanometer scanner-based handheld PAM (WP-GVS-HH-PAM), which provides an extended field of view (14.5 × 9 mm<sup>2</sup>) for wide-range imaging. The fully waterproof handheld probe enables free movement for imaging regardless of sample shape, and volume rate and scanning region are adjustable per experimental conditions. Results of WP-GVS-HH-PAM-based phantom and in vivo imaging of mouse tissues (ear, iris, and brain) confirm the feasibility and applicability of our system as an imaging modality for various biomedical applications. |
| first_indexed | 2024-03-10T08:28:25Z |
| format | Article |
| id | doaj.art-83cae91e58424276ba51a0b2f8c7ad6e |
| institution | Directory Open Access Journal |
| issn | 2304-6732 |
| language | English |
| last_indexed | 2024-03-10T08:28:25Z |
| publishDate | 2021-07-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Photonics |
| spelling | doaj.art-83cae91e58424276ba51a0b2f8c7ad6e2023-11-22T09:16:20ZengMDPI AGPhotonics2304-67322021-07-018830510.3390/photonics8080305Waterproof Galvanometer Scanner-Based Handheld Photoacoustic Microscopy Probe for Wide-Field Vasculature Imaging In VivoDaewoon Seong0Sangyeob Han1Jaeyul Lee2Euimin Lee3Yoonseok Kim4Junsoo Lee5Mansik Jeon6Jeehyun Kim7School of Electronic and Electrical Engineering, College of IT Engineering, Kyungpook National University, Daegu 41566, KoreaSchool of Electronic and Electrical Engineering, College of IT Engineering, Kyungpook National University, Daegu 41566, KoreaSchool of Electronic and Electrical Engineering, College of IT Engineering, Kyungpook National University, Daegu 41566, KoreaSchool of Electronic and Electrical Engineering, College of IT Engineering, Kyungpook National University, Daegu 41566, KoreaSchool of Electronic and Electrical Engineering, College of IT Engineering, Kyungpook National University, Daegu 41566, KoreaSchool of Electronic and Electrical Engineering, College of IT Engineering, Kyungpook National University, Daegu 41566, KoreaSchool of Electronic and Electrical Engineering, College of IT Engineering, Kyungpook National University, Daegu 41566, KoreaSchool of Electronic and Electrical Engineering, College of IT Engineering, Kyungpook National University, Daegu 41566, KoreaPhotoacoustic imaging (PAI) is a hybrid non-invasive imaging technique used to merge high optical contrast and high acoustic resolution in deep tissue. PAI has been extensively developed by utilizing its advantages that include deep imaging depth, high resolution, and label-free imaging. As a representative implementation of PAI, photoacoustic microscopy (PAM) has been used in preclinical and clinical studies for its micron-scale spatial resolution capability with high optical absorption contrast. Several handheld and portable PAM systems have been developed that improve its applicability to several fields, making it versatile. In this study, we developed a laboratory-customized, two-axis, waterproof, galvanometer scanner-based handheld PAM (WP-GVS-HH-PAM), which provides an extended field of view (14.5 × 9 mm<sup>2</sup>) for wide-range imaging. The fully waterproof handheld probe enables free movement for imaging regardless of sample shape, and volume rate and scanning region are adjustable per experimental conditions. Results of WP-GVS-HH-PAM-based phantom and in vivo imaging of mouse tissues (ear, iris, and brain) confirm the feasibility and applicability of our system as an imaging modality for various biomedical applications.https://www.mdpi.com/2304-6732/8/8/305photoacoustic microscopyhandheld probewide-field imagingin vivo vasculature imaging3D imaging |
| spellingShingle | Daewoon Seong Sangyeob Han Jaeyul Lee Euimin Lee Yoonseok Kim Junsoo Lee Mansik Jeon Jeehyun Kim Waterproof Galvanometer Scanner-Based Handheld Photoacoustic Microscopy Probe for Wide-Field Vasculature Imaging In Vivo Photonics photoacoustic microscopy handheld probe wide-field imaging in vivo vasculature imaging 3D imaging |
| title | Waterproof Galvanometer Scanner-Based Handheld Photoacoustic Microscopy Probe for Wide-Field Vasculature Imaging In Vivo |
| title_full | Waterproof Galvanometer Scanner-Based Handheld Photoacoustic Microscopy Probe for Wide-Field Vasculature Imaging In Vivo |
| title_fullStr | Waterproof Galvanometer Scanner-Based Handheld Photoacoustic Microscopy Probe for Wide-Field Vasculature Imaging In Vivo |
| title_full_unstemmed | Waterproof Galvanometer Scanner-Based Handheld Photoacoustic Microscopy Probe for Wide-Field Vasculature Imaging In Vivo |
| title_short | Waterproof Galvanometer Scanner-Based Handheld Photoacoustic Microscopy Probe for Wide-Field Vasculature Imaging In Vivo |
| title_sort | waterproof galvanometer scanner based handheld photoacoustic microscopy probe for wide field vasculature imaging in vivo |
| topic | photoacoustic microscopy handheld probe wide-field imaging in vivo vasculature imaging 3D imaging |
| url | https://www.mdpi.com/2304-6732/8/8/305 |
| work_keys_str_mv | AT daewoonseong waterproofgalvanometerscannerbasedhandheldphotoacousticmicroscopyprobeforwidefieldvasculatureimaginginvivo AT sangyeobhan waterproofgalvanometerscannerbasedhandheldphotoacousticmicroscopyprobeforwidefieldvasculatureimaginginvivo AT jaeyullee waterproofgalvanometerscannerbasedhandheldphotoacousticmicroscopyprobeforwidefieldvasculatureimaginginvivo AT euiminlee waterproofgalvanometerscannerbasedhandheldphotoacousticmicroscopyprobeforwidefieldvasculatureimaginginvivo AT yoonseokkim waterproofgalvanometerscannerbasedhandheldphotoacousticmicroscopyprobeforwidefieldvasculatureimaginginvivo AT junsoolee waterproofgalvanometerscannerbasedhandheldphotoacousticmicroscopyprobeforwidefieldvasculatureimaginginvivo AT mansikjeon waterproofgalvanometerscannerbasedhandheldphotoacousticmicroscopyprobeforwidefieldvasculatureimaginginvivo AT jeehyunkim waterproofgalvanometerscannerbasedhandheldphotoacousticmicroscopyprobeforwidefieldvasculatureimaginginvivo |