Exopolysaccharides of Bifidobacterium longum subsp. infantis E4 on the immune and anti-inflammatory effects in vitro
Exopolysaccharides (EPS) are biological macromolecules produced by Bifidobacterium to the outside of the cell wall during growth and metabolism. The EPS of Bifidobacterium have anti-inflammatory and antimicrobial activities and could regulate the immune system. In this study, the Bifidobacterium lon...
| Main Authors: | , , , , , , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
Elsevier
2023-08-01
|
| Series: | Journal of Functional Foods |
| Subjects: | |
| Online Access: | http://www.sciencedirect.com/science/article/pii/S1756464623002992 |
| _version_ | 1827871769098190848 |
|---|---|
| author | Yingxue Yue Yuqi Wang Rui Yang Deyu Liu Yang Cheng Shuang Li Lenan Sun Bailiang Li Guicheng Huo |
| author_facet | Yingxue Yue Yuqi Wang Rui Yang Deyu Liu Yang Cheng Shuang Li Lenan Sun Bailiang Li Guicheng Huo |
| author_sort | Yingxue Yue |
| collection | DOAJ |
| description | Exopolysaccharides (EPS) are biological macromolecules produced by Bifidobacterium to the outside of the cell wall during growth and metabolism. The EPS of Bifidobacterium have anti-inflammatory and antimicrobial activities and could regulate the immune system. In this study, the Bifidobacterium longum subsp. infantis E4 (B. infantis E4) has 21 specific genes for carbohydrate transport and metabolism compared with other 12 strains of B. infantis and could produce exopolysaccharides. The average molecular weights of EPS-1, EPS-2 and EPS-3 were 2.68 × 104, 3.79 × 104 and 4.72 × 104 Da, respectively. The EPS-1 mainly comprised of 7 kinds of monosaccharide, and EPS-2 and EPS-3 mainly comprised of 8 kinds of monosaccharide. The immunomodulatory and anti-inflammatory properties of EPS-1, EPS-2 and EPS-3 on RAW264.7 cells, spleen lymphocytes and NK cells of mouse were investigated in vitro of this study. EPS-1, EPS-2 and EPS-3 promoted the growth and increased phagocytic index of macrophage RAW264.7 at the concentration of 100 μg/mL. Subsequently, EPS-1, EPS-2 and EPS-3 groups were found to increase splenic lymphocyte proliferation and NK cell activity. Compared with the LPS group, EPS-1, EPS-2 and EPS-3 reduced the production of NO, IL-1β, IL-6, TNF-α and PGE-2 in RAW264.7 cells after in lipopolysaccharide (LPS) induced, with EPS-1 having the best effect. Meanwhile, EPS-1, EPS-2 and EPS-3 down-regulated the expression levels of LPS-induced NF-κβ signaling pathway-related genes p-p65, p-Iκβ, iNOS, COX-2 and reduced the nuclear translocation of p65 protein in RAW264.7 cells. In conclusion, our study suggested that EPS produced by B. infantis E4 has immunostimulatory and anti-inflammatory activities and may serve as a potential prebiotic to maintain future health. |
| first_indexed | 2024-03-12T16:15:35Z |
| format | Article |
| id | doaj.art-83cc36f0705e440dbd076f9c050beb5c |
| institution | Directory Open Access Journal |
| issn | 1756-4646 |
| language | English |
| last_indexed | 2024-03-12T16:15:35Z |
| publishDate | 2023-08-01 |
| publisher | Elsevier |
| record_format | Article |
| series | Journal of Functional Foods |
| spelling | doaj.art-83cc36f0705e440dbd076f9c050beb5c2023-08-09T04:32:38ZengElsevierJournal of Functional Foods1756-46462023-08-01107105699Exopolysaccharides of Bifidobacterium longum subsp. infantis E4 on the immune and anti-inflammatory effects in vitroYingxue Yue0Yuqi Wang1Rui Yang2Deyu Liu3Yang Cheng4Shuang Li5Lenan Sun6Bailiang Li7Guicheng Huo8Key Laboratory of Dairy Science, Ministry of Education, Northeast Agricultural University, Harbin 150030, China; Food College, Northeast Agricultural University, Harbin 150030, ChinaHeilongjiang Jinxiang Biochemical Co., LTD, Harbin 150030, ChinaKey Laboratory of Dairy Science, Ministry of Education, Northeast Agricultural University, Harbin 150030, China; Food College, Northeast Agricultural University, Harbin 150030, ChinaKey Laboratory of Dairy Science, Ministry of Education, Northeast Agricultural University, Harbin 150030, China; Food College, Northeast Agricultural University, Harbin 150030, ChinaKey Laboratory of Dairy Science, Ministry of Education, Northeast Agricultural University, Harbin 150030, China; Food College, Northeast Agricultural University, Harbin 150030, ChinaKey Laboratory of Dairy Science, Ministry of Education, Northeast Agricultural University, Harbin 150030, China; Food College, Northeast Agricultural University, Harbin 150030, ChinaKey Laboratory of Dairy Science, Ministry of Education, Northeast Agricultural University, Harbin 150030, China; Food College, Northeast Agricultural University, Harbin 150030, ChinaKey Laboratory of Dairy Science, Ministry of Education, Northeast Agricultural University, Harbin 150030, China; Food College, Northeast Agricultural University, Harbin 150030, China; Corresponding authors at: Key Laboratory of Dairy Science, Ministry of Education, Northeast Agricultural University, Harbin 150030, China.Key Laboratory of Dairy Science, Ministry of Education, Northeast Agricultural University, Harbin 150030, China; Food College, Northeast Agricultural University, Harbin 150030, China; Corresponding authors at: Key Laboratory of Dairy Science, Ministry of Education, Northeast Agricultural University, Harbin 150030, China.Exopolysaccharides (EPS) are biological macromolecules produced by Bifidobacterium to the outside of the cell wall during growth and metabolism. The EPS of Bifidobacterium have anti-inflammatory and antimicrobial activities and could regulate the immune system. In this study, the Bifidobacterium longum subsp. infantis E4 (B. infantis E4) has 21 specific genes for carbohydrate transport and metabolism compared with other 12 strains of B. infantis and could produce exopolysaccharides. The average molecular weights of EPS-1, EPS-2 and EPS-3 were 2.68 × 104, 3.79 × 104 and 4.72 × 104 Da, respectively. The EPS-1 mainly comprised of 7 kinds of monosaccharide, and EPS-2 and EPS-3 mainly comprised of 8 kinds of monosaccharide. The immunomodulatory and anti-inflammatory properties of EPS-1, EPS-2 and EPS-3 on RAW264.7 cells, spleen lymphocytes and NK cells of mouse were investigated in vitro of this study. EPS-1, EPS-2 and EPS-3 promoted the growth and increased phagocytic index of macrophage RAW264.7 at the concentration of 100 μg/mL. Subsequently, EPS-1, EPS-2 and EPS-3 groups were found to increase splenic lymphocyte proliferation and NK cell activity. Compared with the LPS group, EPS-1, EPS-2 and EPS-3 reduced the production of NO, IL-1β, IL-6, TNF-α and PGE-2 in RAW264.7 cells after in lipopolysaccharide (LPS) induced, with EPS-1 having the best effect. Meanwhile, EPS-1, EPS-2 and EPS-3 down-regulated the expression levels of LPS-induced NF-κβ signaling pathway-related genes p-p65, p-Iκβ, iNOS, COX-2 and reduced the nuclear translocation of p65 protein in RAW264.7 cells. In conclusion, our study suggested that EPS produced by B. infantis E4 has immunostimulatory and anti-inflammatory activities and may serve as a potential prebiotic to maintain future health.http://www.sciencedirect.com/science/article/pii/S1756464623002992BifidobacteriumComparative genomeExopolysaccharidesAnti-inflammatoryRaw 264.7 cells |
| spellingShingle | Yingxue Yue Yuqi Wang Rui Yang Deyu Liu Yang Cheng Shuang Li Lenan Sun Bailiang Li Guicheng Huo Exopolysaccharides of Bifidobacterium longum subsp. infantis E4 on the immune and anti-inflammatory effects in vitro Journal of Functional Foods Bifidobacterium Comparative genome Exopolysaccharides Anti-inflammatory Raw 264.7 cells |
| title | Exopolysaccharides of Bifidobacterium longum subsp. infantis E4 on the immune and anti-inflammatory effects in vitro |
| title_full | Exopolysaccharides of Bifidobacterium longum subsp. infantis E4 on the immune and anti-inflammatory effects in vitro |
| title_fullStr | Exopolysaccharides of Bifidobacterium longum subsp. infantis E4 on the immune and anti-inflammatory effects in vitro |
| title_full_unstemmed | Exopolysaccharides of Bifidobacterium longum subsp. infantis E4 on the immune and anti-inflammatory effects in vitro |
| title_short | Exopolysaccharides of Bifidobacterium longum subsp. infantis E4 on the immune and anti-inflammatory effects in vitro |
| title_sort | exopolysaccharides of bifidobacterium longum subsp infantis e4 on the immune and anti inflammatory effects in vitro |
| topic | Bifidobacterium Comparative genome Exopolysaccharides Anti-inflammatory Raw 264.7 cells |
| url | http://www.sciencedirect.com/science/article/pii/S1756464623002992 |
| work_keys_str_mv | AT yingxueyue exopolysaccharidesofbifidobacteriumlongumsubspinfantise4ontheimmuneandantiinflammatoryeffectsinvitro AT yuqiwang exopolysaccharidesofbifidobacteriumlongumsubspinfantise4ontheimmuneandantiinflammatoryeffectsinvitro AT ruiyang exopolysaccharidesofbifidobacteriumlongumsubspinfantise4ontheimmuneandantiinflammatoryeffectsinvitro AT deyuliu exopolysaccharidesofbifidobacteriumlongumsubspinfantise4ontheimmuneandantiinflammatoryeffectsinvitro AT yangcheng exopolysaccharidesofbifidobacteriumlongumsubspinfantise4ontheimmuneandantiinflammatoryeffectsinvitro AT shuangli exopolysaccharidesofbifidobacteriumlongumsubspinfantise4ontheimmuneandantiinflammatoryeffectsinvitro AT lenansun exopolysaccharidesofbifidobacteriumlongumsubspinfantise4ontheimmuneandantiinflammatoryeffectsinvitro AT bailiangli exopolysaccharidesofbifidobacteriumlongumsubspinfantise4ontheimmuneandantiinflammatoryeffectsinvitro AT guichenghuo exopolysaccharidesofbifidobacteriumlongumsubspinfantise4ontheimmuneandantiinflammatoryeffectsinvitro |