Homocysteine exchange across skeletal muscle in patients with chronic kidney disease
Abstract Sites and mechanisms regulating the supply of homocysteine (Hcy) to the circulation are unexplored in humans. We studied the exchange of Hcy across the forearm in CKD patients (n = 17, eGFR 20 ± 2 ml/min), in hemodialysis (HD)‐treated patients (n = 14) and controls (n = 9). Arterial Hcy was...
| Main Authors: | , , , , , , , , |
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| Format: | Article |
| Language: | English |
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Wiley
2023-03-01
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| Series: | Physiological Reports |
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| Online Access: | https://doi.org/10.14814/phy2.15573 |
| _version_ | 1827397842088493056 |
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| author | Giacomo Garibotto Daniela Picciotto Daniela Verzola Alessando Valli Antonella Sofia Francesca Costigliolo Michela Saio Francesca Viazzi Pasquale Esposito |
| author_facet | Giacomo Garibotto Daniela Picciotto Daniela Verzola Alessando Valli Antonella Sofia Francesca Costigliolo Michela Saio Francesca Viazzi Pasquale Esposito |
| author_sort | Giacomo Garibotto |
| collection | DOAJ |
| description | Abstract Sites and mechanisms regulating the supply of homocysteine (Hcy) to the circulation are unexplored in humans. We studied the exchange of Hcy across the forearm in CKD patients (n = 17, eGFR 20 ± 2 ml/min), in hemodialysis (HD)‐treated patients (n = 14) and controls (n = 9). Arterial Hcy was ~ 2.5 folds increased in CKD and HD patients (p < 0.05–0.03 vs. controls). Both in controls and in patients Hcy levels in the deep forearm vein were consistently greater (+~7%, p < 0.05–0.01) than the corresponding arterial levels, indicating the occurrence of Hcy release from muscle. The release of Hcy from the forearm was similar among groups. In all groups arterial Hcy varied with its release from muscle (p < 0.03–0.02), suggesting that muscle plays an important role on plasma Hcy levels. Forearm Hcy release was inversely related to folate plasma level in all study groups but neither to vitamin B12 and IL‐6 levels nor to muscle protein net balance. These data indicate that the release of Hcy from peripheral tissue metabolism plays a major role in influencing its Hcy plasma levels in humans and patients with CKD, and that folate is a major determinant of Hcy release. |
| first_indexed | 2024-03-08T19:17:37Z |
| format | Article |
| id | doaj.art-83cd5c82610e4cd6892a0e50ca81042f |
| institution | Directory Open Access Journal |
| issn | 2051-817X |
| language | English |
| last_indexed | 2024-03-08T19:17:37Z |
| publishDate | 2023-03-01 |
| publisher | Wiley |
| record_format | Article |
| series | Physiological Reports |
| spelling | doaj.art-83cd5c82610e4cd6892a0e50ca81042f2023-12-27T04:12:43ZengWileyPhysiological Reports2051-817X2023-03-01116n/an/a10.14814/phy2.15573Homocysteine exchange across skeletal muscle in patients with chronic kidney diseaseGiacomo Garibotto0Daniela Picciotto1Daniela Verzola2Alessando Valli3Antonella Sofia4Francesca Costigliolo5Michela Saio6Francesca Viazzi7Pasquale Esposito8Department of Internal Medicine University of Genova Genova ItalyDivision of Nephrology, Dialysis and Transplantation, IRCCS Ospedale Policlinico San Martino Genoa ItalyDepartment of Internal Medicine University of Genova Genova ItalyDepartment of Internal Medicine University of Genova Genova ItalyDivision of Nephrology, Dialysis and Transplantation, IRCCS Ospedale Policlinico San Martino Genoa ItalyDivision of Nephrology, Dialysis and Transplantation, IRCCS Ospedale Policlinico San Martino Genoa ItalyDivision of Nephrology, Dialysis and Transplantation, IRCCS Ospedale Policlinico San Martino Genoa ItalyDepartment of Internal Medicine University of Genova Genova ItalyDepartment of Internal Medicine University of Genova Genova ItalyAbstract Sites and mechanisms regulating the supply of homocysteine (Hcy) to the circulation are unexplored in humans. We studied the exchange of Hcy across the forearm in CKD patients (n = 17, eGFR 20 ± 2 ml/min), in hemodialysis (HD)‐treated patients (n = 14) and controls (n = 9). Arterial Hcy was ~ 2.5 folds increased in CKD and HD patients (p < 0.05–0.03 vs. controls). Both in controls and in patients Hcy levels in the deep forearm vein were consistently greater (+~7%, p < 0.05–0.01) than the corresponding arterial levels, indicating the occurrence of Hcy release from muscle. The release of Hcy from the forearm was similar among groups. In all groups arterial Hcy varied with its release from muscle (p < 0.03–0.02), suggesting that muscle plays an important role on plasma Hcy levels. Forearm Hcy release was inversely related to folate plasma level in all study groups but neither to vitamin B12 and IL‐6 levels nor to muscle protein net balance. These data indicate that the release of Hcy from peripheral tissue metabolism plays a major role in influencing its Hcy plasma levels in humans and patients with CKD, and that folate is a major determinant of Hcy release.https://doi.org/10.14814/phy2.15573CKDfolatehomocysteinemethioninemuscle |
| spellingShingle | Giacomo Garibotto Daniela Picciotto Daniela Verzola Alessando Valli Antonella Sofia Francesca Costigliolo Michela Saio Francesca Viazzi Pasquale Esposito Homocysteine exchange across skeletal muscle in patients with chronic kidney disease Physiological Reports CKD folate homocysteine methionine muscle |
| title | Homocysteine exchange across skeletal muscle in patients with chronic kidney disease |
| title_full | Homocysteine exchange across skeletal muscle in patients with chronic kidney disease |
| title_fullStr | Homocysteine exchange across skeletal muscle in patients with chronic kidney disease |
| title_full_unstemmed | Homocysteine exchange across skeletal muscle in patients with chronic kidney disease |
| title_short | Homocysteine exchange across skeletal muscle in patients with chronic kidney disease |
| title_sort | homocysteine exchange across skeletal muscle in patients with chronic kidney disease |
| topic | CKD folate homocysteine methionine muscle |
| url | https://doi.org/10.14814/phy2.15573 |
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