Preliminary insights on the metabolomics of Trichinella zimbabwensis infection in Sprague Dawley rats using GCxGC-TOF-MS (untargeted approach)
Trichinella infections have been documented globally and have been detected in wild and/or domestic animals except Antarctica. There is paucity of information in the metabolic responses of hosts during Trichinella infections and biomarkers for infection that can be used in the diagnosis of the disea...
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Frontiers Media S.A.
2023-02-01
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| Series: | Frontiers in Molecular Biosciences |
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| Online Access: | https://www.frontiersin.org/articles/10.3389/fmolb.2023.1128542/full |
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| author | I. S. Ndlovu Ekuyikeno Silas S. I. Tshilwane M. Chaisi M. Chaisi A. Vosloo S. Mukaratirwa S. Mukaratirwa |
| author_facet | I. S. Ndlovu Ekuyikeno Silas S. I. Tshilwane M. Chaisi M. Chaisi A. Vosloo S. Mukaratirwa S. Mukaratirwa |
| author_sort | I. S. Ndlovu |
| collection | DOAJ |
| description | Trichinella infections have been documented globally and have been detected in wild and/or domestic animals except Antarctica. There is paucity of information in the metabolic responses of hosts during Trichinella infections and biomarkers for infection that can be used in the diagnosis of the disease. The current study aimed to apply a non-targeted metabolomic approach to identify Trichinella zimbabwensis biomarkers including metabolic response from sera of infected Sprague-Dawley rats. Fifty-four male Sprague-Dawley rats were randomly assigned into T. zimbabwensis infected group (n = 36) and the non-infected control (n = 18). Results from the study showed that the metabolic signature of T. zimbabwensis infection consists of enriched methyl histidine metabolism, disturbance of the liver urea cycle, impeded TCA cycle, and upregulation of gluconeogenesis metabolism. The observed disturbance in the metabolic pathways was attributed to the effects caused by the parasite during its migration to the muscles resulting in downregulation of amino acids intermediates in the Trichinella-infected animals, and therefore affecting energy production and degradation of biomolecules. It was concluded that T. zimbabwensis infection caused an upregulation of amino acids; pipecolic acid, histidine, and urea, and upregulation of glucose and meso-Erythritol. Moreover, T. zimbabwensis infection caused upregulation of the fatty acids, retinoic acid, and acetic acid. These findings highlight the potential of metabolomics as a novel approach for fundamental investigations of host-pathogen interactions as well as for disease progression and prognosis. |
| first_indexed | 2024-04-10T09:38:45Z |
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| issn | 2296-889X |
| language | English |
| last_indexed | 2024-04-10T09:38:45Z |
| publishDate | 2023-02-01 |
| publisher | Frontiers Media S.A. |
| record_format | Article |
| series | Frontiers in Molecular Biosciences |
| spelling | doaj.art-83ce99251df04714a012b132b7ed4c272023-02-17T14:11:13ZengFrontiers Media S.A.Frontiers in Molecular Biosciences2296-889X2023-02-011010.3389/fmolb.2023.11285421128542Preliminary insights on the metabolomics of Trichinella zimbabwensis infection in Sprague Dawley rats using GCxGC-TOF-MS (untargeted approach)I. S. Ndlovu0Ekuyikeno Silas1S. I. Tshilwane2M. Chaisi3M. Chaisi4A. Vosloo5S. Mukaratirwa6S. Mukaratirwa7School of Life Sciences, University of KwaZulu-Natal, Westville Campus, Durban, South AfricaSchool of Life Sciences, University of KwaZulu-Natal, Westville Campus, Durban, South AfricaDepartment of Veterinary Tropical Diseases, Faculty of Veterinary Science, University of Pretoria, Pretoria, South AfricaDepartment of Veterinary Tropical Diseases, Faculty of Veterinary Science, University of Pretoria, Pretoria, South AfricaFoundational Biodiversity Science, South African National Biodiversity Institute, Pretoria, South AfricaSchool of Life Sciences, University of KwaZulu-Natal, Westville Campus, Durban, South AfricaSchool of Life Sciences, University of KwaZulu-Natal, Westville Campus, Durban, South AfricaOne Health Center for Zoonoses and Tropical Veterinary Medicine, School of Veterinary Medicine, Ross University, Basseterre, Saint Kitts and NevisTrichinella infections have been documented globally and have been detected in wild and/or domestic animals except Antarctica. There is paucity of information in the metabolic responses of hosts during Trichinella infections and biomarkers for infection that can be used in the diagnosis of the disease. The current study aimed to apply a non-targeted metabolomic approach to identify Trichinella zimbabwensis biomarkers including metabolic response from sera of infected Sprague-Dawley rats. Fifty-four male Sprague-Dawley rats were randomly assigned into T. zimbabwensis infected group (n = 36) and the non-infected control (n = 18). Results from the study showed that the metabolic signature of T. zimbabwensis infection consists of enriched methyl histidine metabolism, disturbance of the liver urea cycle, impeded TCA cycle, and upregulation of gluconeogenesis metabolism. The observed disturbance in the metabolic pathways was attributed to the effects caused by the parasite during its migration to the muscles resulting in downregulation of amino acids intermediates in the Trichinella-infected animals, and therefore affecting energy production and degradation of biomolecules. It was concluded that T. zimbabwensis infection caused an upregulation of amino acids; pipecolic acid, histidine, and urea, and upregulation of glucose and meso-Erythritol. Moreover, T. zimbabwensis infection caused upregulation of the fatty acids, retinoic acid, and acetic acid. These findings highlight the potential of metabolomics as a novel approach for fundamental investigations of host-pathogen interactions as well as for disease progression and prognosis.https://www.frontiersin.org/articles/10.3389/fmolb.2023.1128542/fullgas chromatographic time of flight mass spectrometry (GCxGC-TOF/MS)trichinellosismetabolomicsserumTrichinella zimbabwensis |
| spellingShingle | I. S. Ndlovu Ekuyikeno Silas S. I. Tshilwane M. Chaisi M. Chaisi A. Vosloo S. Mukaratirwa S. Mukaratirwa Preliminary insights on the metabolomics of Trichinella zimbabwensis infection in Sprague Dawley rats using GCxGC-TOF-MS (untargeted approach) Frontiers in Molecular Biosciences gas chromatographic time of flight mass spectrometry (GCxGC-TOF/MS) trichinellosis metabolomics serum Trichinella zimbabwensis |
| title | Preliminary insights on the metabolomics of Trichinella zimbabwensis infection in Sprague Dawley rats using GCxGC-TOF-MS (untargeted approach) |
| title_full | Preliminary insights on the metabolomics of Trichinella zimbabwensis infection in Sprague Dawley rats using GCxGC-TOF-MS (untargeted approach) |
| title_fullStr | Preliminary insights on the metabolomics of Trichinella zimbabwensis infection in Sprague Dawley rats using GCxGC-TOF-MS (untargeted approach) |
| title_full_unstemmed | Preliminary insights on the metabolomics of Trichinella zimbabwensis infection in Sprague Dawley rats using GCxGC-TOF-MS (untargeted approach) |
| title_short | Preliminary insights on the metabolomics of Trichinella zimbabwensis infection in Sprague Dawley rats using GCxGC-TOF-MS (untargeted approach) |
| title_sort | preliminary insights on the metabolomics of trichinella zimbabwensis infection in sprague dawley rats using gcxgc tof ms untargeted approach |
| topic | gas chromatographic time of flight mass spectrometry (GCxGC-TOF/MS) trichinellosis metabolomics serum Trichinella zimbabwensis |
| url | https://www.frontiersin.org/articles/10.3389/fmolb.2023.1128542/full |
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