In Patients Treated by Selective Internal Radiotherapy, Cellular In Vitro Immune Function Is Predictive of Survival
In patients with liver malignancies, the cellular immune function was impaired in vitro after selective internal radiotherapy (SIRT). Because immunosuppression varied substantially, in the current study, we investigated in 25 SIRT patients followed up for ten years whether the lymphocyte function wa...
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| Format: | Article |
| Language: | English |
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MDPI AG
2023-08-01
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| Series: | Cancers |
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| Online Access: | https://www.mdpi.com/2072-6694/15/16/4055 |
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| author | Aglaia Domouchtsidou Ferdinand Beckmann Beate Marenbach Stefan P. Mueller Jan Best Ken Herrmann Peter A. Horn Vahé Barsegian Monika Lindemann |
| author_facet | Aglaia Domouchtsidou Ferdinand Beckmann Beate Marenbach Stefan P. Mueller Jan Best Ken Herrmann Peter A. Horn Vahé Barsegian Monika Lindemann |
| author_sort | Aglaia Domouchtsidou |
| collection | DOAJ |
| description | In patients with liver malignancies, the cellular immune function was impaired in vitro after selective internal radiotherapy (SIRT). Because immunosuppression varied substantially, in the current study, we investigated in 25 SIRT patients followed up for ten years whether the lymphocyte function was correlated with survival. Peripheral blood mononuclear cells were stimulated with four microbial antigens (tuberculin, tetanus toxoid, <i>Candida albicans</i> and CMV) before therapy and at four time points thereafter, and lymphocyte proliferation was determined by H3-thymidine uptake. The median sum of the responses to these four antigens decreased from 39,464 counts per minute (CPM) increment (range 1080–204,512) before therapy to a minimum of 700 CPM increment on day 7 after therapy (0–93,187, <i>p</i> < 0.0001). At all five time points, the median survival in patients with weaker responses was 2- to 3.5-fold shorter (<i>p</i> < 0.05). On day 7, the median survival in patients with responses below and above the cutoff of a 2 CPM increment was 185 and 523 days, respectively (χ<sup>2</sup> = 9.4, <i>p</i> = 0.002). In conclusion, lymphocyte function could be a new predictor of treatment outcome after SIRT. |
| first_indexed | 2024-03-11T00:03:26Z |
| format | Article |
| id | doaj.art-83d14b856a284bc193aecd0b0ba9e601 |
| institution | Directory Open Access Journal |
| issn | 2072-6694 |
| language | English |
| last_indexed | 2024-03-11T00:03:26Z |
| publishDate | 2023-08-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Cancers |
| spelling | doaj.art-83d14b856a284bc193aecd0b0ba9e6012023-11-19T00:32:31ZengMDPI AGCancers2072-66942023-08-011516405510.3390/cancers15164055In Patients Treated by Selective Internal Radiotherapy, Cellular In Vitro Immune Function Is Predictive of SurvivalAglaia Domouchtsidou0Ferdinand Beckmann1Beate Marenbach2Stefan P. Mueller3Jan Best4Ken Herrmann5Peter A. Horn6Vahé Barsegian7Monika Lindemann8Institute for Transfusion Medicine, University Hospital Essen, University of Duisburg-Essen, Virchowstraße 179, 45147 Essen, GermanyInstitute for Transfusion Medicine, University Hospital Essen, University of Duisburg-Essen, Virchowstraße 179, 45147 Essen, GermanyInstitute for Transfusion Medicine, University Hospital Essen, University of Duisburg-Essen, Virchowstraße 179, 45147 Essen, GermanyDepartment of Nuclear Medicine, University Hospital Essen, University of Duisburg-Essen, 45147 Essen, GermanyDepartment of Gastroenterology and Hepatology, University Hospital Essen, University of Duisburg-Essen, 45147 Essen, GermanyDepartment of Nuclear Medicine, University Hospital Essen, University of Duisburg-Essen, 45147 Essen, GermanyInstitute for Transfusion Medicine, University Hospital Essen, University of Duisburg-Essen, Virchowstraße 179, 45147 Essen, GermanyDepartment of Nuclear Medicine, University Hospital Essen, University of Duisburg-Essen, 45147 Essen, GermanyInstitute for Transfusion Medicine, University Hospital Essen, University of Duisburg-Essen, Virchowstraße 179, 45147 Essen, GermanyIn patients with liver malignancies, the cellular immune function was impaired in vitro after selective internal radiotherapy (SIRT). Because immunosuppression varied substantially, in the current study, we investigated in 25 SIRT patients followed up for ten years whether the lymphocyte function was correlated with survival. Peripheral blood mononuclear cells were stimulated with four microbial antigens (tuberculin, tetanus toxoid, <i>Candida albicans</i> and CMV) before therapy and at four time points thereafter, and lymphocyte proliferation was determined by H3-thymidine uptake. The median sum of the responses to these four antigens decreased from 39,464 counts per minute (CPM) increment (range 1080–204,512) before therapy to a minimum of 700 CPM increment on day 7 after therapy (0–93,187, <i>p</i> < 0.0001). At all five time points, the median survival in patients with weaker responses was 2- to 3.5-fold shorter (<i>p</i> < 0.05). On day 7, the median survival in patients with responses below and above the cutoff of a 2 CPM increment was 185 and 523 days, respectively (χ<sup>2</sup> = 9.4, <i>p</i> = 0.002). In conclusion, lymphocyte function could be a new predictor of treatment outcome after SIRT.https://www.mdpi.com/2072-6694/15/16/4055selective internal radiotherapylymphocyte proliferationELISpotinterferon-gammainterleukin-2patient survival |
| spellingShingle | Aglaia Domouchtsidou Ferdinand Beckmann Beate Marenbach Stefan P. Mueller Jan Best Ken Herrmann Peter A. Horn Vahé Barsegian Monika Lindemann In Patients Treated by Selective Internal Radiotherapy, Cellular In Vitro Immune Function Is Predictive of Survival Cancers selective internal radiotherapy lymphocyte proliferation ELISpot interferon-gamma interleukin-2 patient survival |
| title | In Patients Treated by Selective Internal Radiotherapy, Cellular In Vitro Immune Function Is Predictive of Survival |
| title_full | In Patients Treated by Selective Internal Radiotherapy, Cellular In Vitro Immune Function Is Predictive of Survival |
| title_fullStr | In Patients Treated by Selective Internal Radiotherapy, Cellular In Vitro Immune Function Is Predictive of Survival |
| title_full_unstemmed | In Patients Treated by Selective Internal Radiotherapy, Cellular In Vitro Immune Function Is Predictive of Survival |
| title_short | In Patients Treated by Selective Internal Radiotherapy, Cellular In Vitro Immune Function Is Predictive of Survival |
| title_sort | in patients treated by selective internal radiotherapy cellular in vitro immune function is predictive of survival |
| topic | selective internal radiotherapy lymphocyte proliferation ELISpot interferon-gamma interleukin-2 patient survival |
| url | https://www.mdpi.com/2072-6694/15/16/4055 |
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