Imipenem resistance in clinical Escherichia coli from Qom, Iran
Abstract Objective The emergence of metallo-β-lactamase-producing Enterobacteriaceae is a worldwide health concern. In this study, the first evaluation of MBL genes, bla IMP and bla VIM , in Escherichia coli resistant to imipenem isolated from urine and blood specimens in Qom, Iran is described. Thr...
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| Format: | Article |
| Language: | English |
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BMC
2018-05-01
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| Series: | BMC Research Notes |
| Subjects: | |
| Online Access: | http://link.springer.com/article/10.1186/s13104-018-3406-6 |
| _version_ | 1828396518095192064 |
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| author | Saeed Shams Ali Hashemi Mohammad Esmkhani Somaye Kermani Elham Shams Alessandra Piccirillo |
| author_facet | Saeed Shams Ali Hashemi Mohammad Esmkhani Somaye Kermani Elham Shams Alessandra Piccirillo |
| author_sort | Saeed Shams |
| collection | DOAJ |
| description | Abstract Objective The emergence of metallo-β-lactamase-producing Enterobacteriaceae is a worldwide health concern. In this study, the first evaluation of MBL genes, bla IMP and bla VIM , in Escherichia coli resistant to imipenem isolated from urine and blood specimens in Qom, Iran is described. Three hundred urine and blood specimens were analysed to detect the presence of E. coli. Resistance to imipenem and other antimicrobials was determined by disk diffusion and MIC. MBL production was screened using CDDT. PCR was also carried out to determine the presence of bla IMP and bla VIM genes in imipenem-resistant isolates. Results In total, 160 E. coli isolates were collected from March to May 2016. According to disk diffusion, high-level of resistance (20%) to cefotaxime was observed, whereas the lowest (1%) was detected for tetracycline. In addition, five isolates showed resistance to imipenem with a MIC ≥ 4 µg/mL. CDDT test confirmed that five isolates were MBL-producing strains, but no bla IMP and bla VIM genes were detected. Results of this study show a very low level of resistance to imipenem in our geographical area. |
| first_indexed | 2024-12-10T08:31:25Z |
| format | Article |
| id | doaj.art-83d3efce6a584e6087eec003af5380e5 |
| institution | Directory Open Access Journal |
| issn | 1756-0500 |
| language | English |
| last_indexed | 2024-12-10T08:31:25Z |
| publishDate | 2018-05-01 |
| publisher | BMC |
| record_format | Article |
| series | BMC Research Notes |
| spelling | doaj.art-83d3efce6a584e6087eec003af5380e52022-12-22T01:56:04ZengBMCBMC Research Notes1756-05002018-05-011111510.1186/s13104-018-3406-6Imipenem resistance in clinical Escherichia coli from Qom, IranSaeed Shams0Ali Hashemi1Mohammad Esmkhani2Somaye Kermani3Elham Shams4Alessandra Piccirillo5Cellular and Molecular Research Center, Qom University of Medical SciencesDepartment of Microbiology, School of Medicine, Shahid Beheshti University of Medical SciencesAli Ebne Abitaleb HospitalCellular and Molecular Research Center, Qom University of Medical SciencesCellular and Molecular Research Center, Qom University of Medical SciencesDepartment of Comparative Biomedicine and Food Science, University of PaduaAbstract Objective The emergence of metallo-β-lactamase-producing Enterobacteriaceae is a worldwide health concern. In this study, the first evaluation of MBL genes, bla IMP and bla VIM , in Escherichia coli resistant to imipenem isolated from urine and blood specimens in Qom, Iran is described. Three hundred urine and blood specimens were analysed to detect the presence of E. coli. Resistance to imipenem and other antimicrobials was determined by disk diffusion and MIC. MBL production was screened using CDDT. PCR was also carried out to determine the presence of bla IMP and bla VIM genes in imipenem-resistant isolates. Results In total, 160 E. coli isolates were collected from March to May 2016. According to disk diffusion, high-level of resistance (20%) to cefotaxime was observed, whereas the lowest (1%) was detected for tetracycline. In addition, five isolates showed resistance to imipenem with a MIC ≥ 4 µg/mL. CDDT test confirmed that five isolates were MBL-producing strains, but no bla IMP and bla VIM genes were detected. Results of this study show a very low level of resistance to imipenem in our geographical area.http://link.springer.com/article/10.1186/s13104-018-3406-6Escherichia coliImipenemMetallo-β-lactamaseResistance |
| spellingShingle | Saeed Shams Ali Hashemi Mohammad Esmkhani Somaye Kermani Elham Shams Alessandra Piccirillo Imipenem resistance in clinical Escherichia coli from Qom, Iran BMC Research Notes Escherichia coli Imipenem Metallo-β-lactamase Resistance |
| title | Imipenem resistance in clinical Escherichia coli from Qom, Iran |
| title_full | Imipenem resistance in clinical Escherichia coli from Qom, Iran |
| title_fullStr | Imipenem resistance in clinical Escherichia coli from Qom, Iran |
| title_full_unstemmed | Imipenem resistance in clinical Escherichia coli from Qom, Iran |
| title_short | Imipenem resistance in clinical Escherichia coli from Qom, Iran |
| title_sort | imipenem resistance in clinical escherichia coli from qom iran |
| topic | Escherichia coli Imipenem Metallo-β-lactamase Resistance |
| url | http://link.springer.com/article/10.1186/s13104-018-3406-6 |
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